Towards bio-encapsulation of chitosan-silver nanocomplex? Impact on malaria mosquito vectors, human breast adenocarcinoma cells (MCF-7) and behavioral traits of non-target fishes
In this study, we synthesized and bio-encapsulated a chitosan-silver nanocomplex (Ch-AgNPs), characterizing it by UV–Vis spectroscopy, FTIR, EDX, SEM, XRD and Zeta potential analyses. The bio-encapsulated chitosan-Ag nanocomplex (BNC) was efficient as scavenger of free radicals (DPPH and ABTS), if c...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Springer Link
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/63589/ http://psasir.upm.edu.my/id/eprint/63589/1/Towards%20bio-encapsulation%20of%20chitosan-silver%20nanocomplex.pdf |
| _version_ | 1848854825555460096 |
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| author | Murugan, Kadarkarai Jaganathan, Anitha Suresh, Udaiyan Rajaganesh, Rajapandian Jayasanthini, Sudalaimani Higuchi, Akon Kumar, Suresh Benelli, Giovanni |
| author_facet | Murugan, Kadarkarai Jaganathan, Anitha Suresh, Udaiyan Rajaganesh, Rajapandian Jayasanthini, Sudalaimani Higuchi, Akon Kumar, Suresh Benelli, Giovanni |
| author_sort | Murugan, Kadarkarai |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | In this study, we synthesized and bio-encapsulated a chitosan-silver nanocomplex (Ch-AgNPs), characterizing it by UV–Vis spectroscopy, FTIR, EDX, SEM, XRD and Zeta potential analyses. The bio-encapsulated chitosan-Ag nanocomplex (BNC) was efficient as scavenger of free radicals (DPPH and ABTS), if compared to Ch-AgNPs. In toxicity assays against breast cancer cells (MCF-7) the BNC triggered apoptotic pathways, leading to a decline of MCF-7 cell viability with IC50 of 17.79 μg/mL after 48 h of exposure. LC50 of BNC on Anopheles stephensi ranged from 54.65 (larva I), to 98.172 ppm (pupa) while Ch-AgNPs LC50 ranged from 4.432 (I) to 7.641 ppm (pupa). In the field, the application of Ch-AgNP (10 × LC50) lead to A. stephensi larval reduction to 86.2, 48.4 and 100% after 24, 48, and 72 h, while the BNC nanocomplex exhibited 68.8, 36.4 and 100% larval reduction, respectively. Both Ch-AgNPs and the BNC reduced longevity and fecundity of A. stephensi. As regards to non-target effects on fish behavioral traits, in standard conditions, Poecilia reticulata predation on A. stephensi larvae was 70.25 (II) and 46.75 larvae per day (III), while post-treatment with sub-lethal doses of BNC, predation was boosted to 88.5 (II) and 70.25 (III) larvae per day. |
| first_indexed | 2025-11-15T11:16:01Z |
| format | Article |
| id | upm-63589 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:16:01Z |
| publishDate | 2017 |
| publisher | Springer Link |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-635892018-11-07T08:16:52Z http://psasir.upm.edu.my/id/eprint/63589/ Towards bio-encapsulation of chitosan-silver nanocomplex? Impact on malaria mosquito vectors, human breast adenocarcinoma cells (MCF-7) and behavioral traits of non-target fishes Murugan, Kadarkarai Jaganathan, Anitha Suresh, Udaiyan Rajaganesh, Rajapandian Jayasanthini, Sudalaimani Higuchi, Akon Kumar, Suresh Benelli, Giovanni In this study, we synthesized and bio-encapsulated a chitosan-silver nanocomplex (Ch-AgNPs), characterizing it by UV–Vis spectroscopy, FTIR, EDX, SEM, XRD and Zeta potential analyses. The bio-encapsulated chitosan-Ag nanocomplex (BNC) was efficient as scavenger of free radicals (DPPH and ABTS), if compared to Ch-AgNPs. In toxicity assays against breast cancer cells (MCF-7) the BNC triggered apoptotic pathways, leading to a decline of MCF-7 cell viability with IC50 of 17.79 μg/mL after 48 h of exposure. LC50 of BNC on Anopheles stephensi ranged from 54.65 (larva I), to 98.172 ppm (pupa) while Ch-AgNPs LC50 ranged from 4.432 (I) to 7.641 ppm (pupa). In the field, the application of Ch-AgNP (10 × LC50) lead to A. stephensi larval reduction to 86.2, 48.4 and 100% after 24, 48, and 72 h, while the BNC nanocomplex exhibited 68.8, 36.4 and 100% larval reduction, respectively. Both Ch-AgNPs and the BNC reduced longevity and fecundity of A. stephensi. As regards to non-target effects on fish behavioral traits, in standard conditions, Poecilia reticulata predation on A. stephensi larvae was 70.25 (II) and 46.75 larvae per day (III), while post-treatment with sub-lethal doses of BNC, predation was boosted to 88.5 (II) and 70.25 (III) larvae per day. Springer Link 2017-01 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/63589/1/Towards%20bio-encapsulation%20of%20chitosan-silver%20nanocomplex.pdf Murugan, Kadarkarai and Jaganathan, Anitha and Suresh, Udaiyan and Rajaganesh, Rajapandian and Jayasanthini, Sudalaimani and Higuchi, Akon and Kumar, Suresh and Benelli, Giovanni (2017) Towards bio-encapsulation of chitosan-silver nanocomplex? Impact on malaria mosquito vectors, human breast adenocarcinoma cells (MCF-7) and behavioral traits of non-target fishes. Journal of Cluster Science, 28 (1). 529 - 550. ISSN 1040-7278; ESSN: 1572-8862 https://link.springer.com/article/10.1007/s10876-016-1129-1 10.1007/s10876-016-1129-1 |
| spellingShingle | Murugan, Kadarkarai Jaganathan, Anitha Suresh, Udaiyan Rajaganesh, Rajapandian Jayasanthini, Sudalaimani Higuchi, Akon Kumar, Suresh Benelli, Giovanni Towards bio-encapsulation of chitosan-silver nanocomplex? Impact on malaria mosquito vectors, human breast adenocarcinoma cells (MCF-7) and behavioral traits of non-target fishes |
| title | Towards bio-encapsulation of chitosan-silver nanocomplex? Impact on malaria mosquito vectors, human breast adenocarcinoma cells (MCF-7) and behavioral traits of non-target fishes |
| title_full | Towards bio-encapsulation of chitosan-silver nanocomplex? Impact on malaria mosquito vectors, human breast adenocarcinoma cells (MCF-7) and behavioral traits of non-target fishes |
| title_fullStr | Towards bio-encapsulation of chitosan-silver nanocomplex? Impact on malaria mosquito vectors, human breast adenocarcinoma cells (MCF-7) and behavioral traits of non-target fishes |
| title_full_unstemmed | Towards bio-encapsulation of chitosan-silver nanocomplex? Impact on malaria mosquito vectors, human breast adenocarcinoma cells (MCF-7) and behavioral traits of non-target fishes |
| title_short | Towards bio-encapsulation of chitosan-silver nanocomplex? Impact on malaria mosquito vectors, human breast adenocarcinoma cells (MCF-7) and behavioral traits of non-target fishes |
| title_sort | towards bio-encapsulation of chitosan-silver nanocomplex? impact on malaria mosquito vectors, human breast adenocarcinoma cells (mcf-7) and behavioral traits of non-target fishes |
| url | http://psasir.upm.edu.my/id/eprint/63589/ http://psasir.upm.edu.my/id/eprint/63589/ http://psasir.upm.edu.my/id/eprint/63589/ http://psasir.upm.edu.my/id/eprint/63589/1/Towards%20bio-encapsulation%20of%20chitosan-silver%20nanocomplex.pdf |