Thiamine biosynthesis gene expression analysis in Elaeis guineensis during interactions with Hendersonia toruloidea
Thiamine plays an indispensable role as a cofactor in many metabolic reactions in all living organisms. The active form, thiamine pyrophosphate (TPP) functions as a cofactor in important metabolic reactions including glycolysis, pentose phosphate pathway and the tricarboxylic acid cycle. Besides tha...
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| Format: | Article |
| Language: | English |
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Malaysian Palm Oil Board
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/63568/ http://psasir.upm.edu.my/id/eprint/63568/1/Thiamine%20biosynthesis%20gene%20expression%20analysis%20in%20Elaeis%20guineensis%20during%20interactions%20with%20Hendersonia%20toruloidea.pdf |
| _version_ | 1848854819863789568 |
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| author | Kamaruddin, Amirah Nor Seman, Idris Abu Yusof, Zetty Norhana Balia |
| author_facet | Kamaruddin, Amirah Nor Seman, Idris Abu Yusof, Zetty Norhana Balia |
| author_sort | Kamaruddin, Amirah Nor |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Thiamine plays an indispensable role as a cofactor in many metabolic reactions in all living organisms. The active form, thiamine pyrophosphate (TPP) functions as a cofactor in important metabolic reactions including glycolysis, pentose phosphate pathway and the tricarboxylic acid cycle. Besides that, thiamine is also associated with the induction of systemic acquired resistance (SAR) in plants. Hendersonia toruloidea is an endophytic fungus originally isolated from oil palm roots which have been shown to have biocontrol activity. Seven months old oil palm seedlings were inoculated with H. toruloidea and the response on the expression of thiamine biosynthesis genes were investigated. Microscopy analysis was performed to visualise the colonisation of the fungus. Ribonucleic acid (RNA) was extracted from oil palm leaves at Day 1, 7, 15 and 30 post-treatment. Quantitative Real-time PCR (qRT-PCR) was performed to measure the level of expression of four key thiamine biosynthesis genes, namely THI4, THIC, TH1 and TPK. The results showed up to 12-fold increase in the expression of all gene transcripts at Day 1 and 7 post-treatment. This work provides first evidence of enhancement of thiamine biosynthesis by endophytic colonisation in oil palm seedlings. |
| first_indexed | 2025-11-15T11:15:56Z |
| format | Article |
| id | upm-63568 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:15:56Z |
| publishDate | 2017 |
| publisher | Malaysian Palm Oil Board |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-635682018-11-05T02:23:42Z http://psasir.upm.edu.my/id/eprint/63568/ Thiamine biosynthesis gene expression analysis in Elaeis guineensis during interactions with Hendersonia toruloidea Kamaruddin, Amirah Nor Seman, Idris Abu Yusof, Zetty Norhana Balia Thiamine plays an indispensable role as a cofactor in many metabolic reactions in all living organisms. The active form, thiamine pyrophosphate (TPP) functions as a cofactor in important metabolic reactions including glycolysis, pentose phosphate pathway and the tricarboxylic acid cycle. Besides that, thiamine is also associated with the induction of systemic acquired resistance (SAR) in plants. Hendersonia toruloidea is an endophytic fungus originally isolated from oil palm roots which have been shown to have biocontrol activity. Seven months old oil palm seedlings were inoculated with H. toruloidea and the response on the expression of thiamine biosynthesis genes were investigated. Microscopy analysis was performed to visualise the colonisation of the fungus. Ribonucleic acid (RNA) was extracted from oil palm leaves at Day 1, 7, 15 and 30 post-treatment. Quantitative Real-time PCR (qRT-PCR) was performed to measure the level of expression of four key thiamine biosynthesis genes, namely THI4, THIC, TH1 and TPK. The results showed up to 12-fold increase in the expression of all gene transcripts at Day 1 and 7 post-treatment. This work provides first evidence of enhancement of thiamine biosynthesis by endophytic colonisation in oil palm seedlings. Malaysian Palm Oil Board 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/63568/1/Thiamine%20biosynthesis%20gene%20expression%20analysis%20in%20Elaeis%20guineensis%20during%20interactions%20with%20Hendersonia%20toruloidea.pdf Kamaruddin, Amirah Nor and Seman, Idris Abu and Yusof, Zetty Norhana Balia (2017) Thiamine biosynthesis gene expression analysis in Elaeis guineensis during interactions with Hendersonia toruloidea. Journal of Oil Palm Research, 29 (2). 218 - 226. ISSN 1511-2780 http://jopr.mpob.gov.my/thiamine-biosynthesis-gene-expression-analysis-in-elaeis-guineensis-during-interactions-with-hendersonia-toruloidea/ 10.21894/jopr.2017.2902.06 |
| spellingShingle | Kamaruddin, Amirah Nor Seman, Idris Abu Yusof, Zetty Norhana Balia Thiamine biosynthesis gene expression analysis in Elaeis guineensis during interactions with Hendersonia toruloidea |
| title | Thiamine biosynthesis gene expression analysis in Elaeis guineensis during interactions with Hendersonia toruloidea |
| title_full | Thiamine biosynthesis gene expression analysis in Elaeis guineensis during interactions with Hendersonia toruloidea |
| title_fullStr | Thiamine biosynthesis gene expression analysis in Elaeis guineensis during interactions with Hendersonia toruloidea |
| title_full_unstemmed | Thiamine biosynthesis gene expression analysis in Elaeis guineensis during interactions with Hendersonia toruloidea |
| title_short | Thiamine biosynthesis gene expression analysis in Elaeis guineensis during interactions with Hendersonia toruloidea |
| title_sort | thiamine biosynthesis gene expression analysis in elaeis guineensis during interactions with hendersonia toruloidea |
| url | http://psasir.upm.edu.my/id/eprint/63568/ http://psasir.upm.edu.my/id/eprint/63568/ http://psasir.upm.edu.my/id/eprint/63568/ http://psasir.upm.edu.my/id/eprint/63568/1/Thiamine%20biosynthesis%20gene%20expression%20analysis%20in%20Elaeis%20guineensis%20during%20interactions%20with%20Hendersonia%20toruloidea.pdf |