Statistical optimization of gelatin immobilisation on modified surface PCL microcarrier to improve PCL microcarrier compatibility
Growing cells on microcarriers may have overcome the limitation of conventional cell culture system. However, the main challenge remains at ensuring the surface biocompatibility with cells. Polycaprolactone (PCL), a biodegradable polymer, has received considerable attention because of its excellent...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Penerbit UTM Press
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/63284/ http://psasir.upm.edu.my/id/eprint/63284/1/Statistical%20optimization%20of%20gelatin%20immobilisation%20on%20modified%20surface%20PCL%20microcarrier%20.pdf |
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| author | Samsudin, Nurhusna Hashim, Yumi Zuhanis Has-Yun Arifin, Mohd Azmir Mel, Maizirwan Mohd. Salleh, Hamzah Nordin, Norshariza Sopyan, Iis Jimat, Dzun Noraini |
| author_facet | Samsudin, Nurhusna Hashim, Yumi Zuhanis Has-Yun Arifin, Mohd Azmir Mel, Maizirwan Mohd. Salleh, Hamzah Nordin, Norshariza Sopyan, Iis Jimat, Dzun Noraini |
| author_sort | Samsudin, Nurhusna |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Growing cells on microcarriers may have overcome the limitation of conventional cell culture system. However, the main challenge remains at ensuring the surface biocompatibility with cells. Polycaprolactone (PCL), a biodegradable polymer, has received considerable attention because of its excellent mechanical properties and degradation kinetics that suit various applications, but its non-polar hydrocarbon moiety renders it sub-optimal for cell attachment. In this present study, the aim was to improve biocompatibility of PCL microcarrier by introducing oxygen functional group via ultraviolet irradiation and ozone aeration (UV/O3 system) to allow covalent immobilization of gelatin on the PCL microcarrier surface. Respond surface methodology was used as a statistical approach to optimized parameters that effect the immobilization of gelatin. The parameters used to maximized amount of gelatin immobilize were the mol ratio of COOH:EDAC, NHS concentration and gelatin concentration. The optimum conditions for maximum amount of gelatin (1797.33 µg/g) on the surface of PCL were as follows: 1.5 of COOH:EDAC ratio, 10 mM NHS concentration and, 80 mg/ml gelatin. The result shows that gelatin coated PCL microcarrier promote more and rapid cell adhesion with density of as compared to raw PCL microcarrier ( and UV/O3 treated PCL microcarrier ( . Therefore, immobilization of gelatin with optimized parameters onto PCL microcarrier improved biocompatibility of PCL microcarrier. |
| first_indexed | 2025-11-15T11:14:54Z |
| format | Article |
| id | upm-63284 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:14:54Z |
| publishDate | 2017 |
| publisher | Penerbit UTM Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-632842018-09-26T01:55:38Z http://psasir.upm.edu.my/id/eprint/63284/ Statistical optimization of gelatin immobilisation on modified surface PCL microcarrier to improve PCL microcarrier compatibility Samsudin, Nurhusna Hashim, Yumi Zuhanis Has-Yun Arifin, Mohd Azmir Mel, Maizirwan Mohd. Salleh, Hamzah Nordin, Norshariza Sopyan, Iis Jimat, Dzun Noraini Growing cells on microcarriers may have overcome the limitation of conventional cell culture system. However, the main challenge remains at ensuring the surface biocompatibility with cells. Polycaprolactone (PCL), a biodegradable polymer, has received considerable attention because of its excellent mechanical properties and degradation kinetics that suit various applications, but its non-polar hydrocarbon moiety renders it sub-optimal for cell attachment. In this present study, the aim was to improve biocompatibility of PCL microcarrier by introducing oxygen functional group via ultraviolet irradiation and ozone aeration (UV/O3 system) to allow covalent immobilization of gelatin on the PCL microcarrier surface. Respond surface methodology was used as a statistical approach to optimized parameters that effect the immobilization of gelatin. The parameters used to maximized amount of gelatin immobilize were the mol ratio of COOH:EDAC, NHS concentration and gelatin concentration. The optimum conditions for maximum amount of gelatin (1797.33 µg/g) on the surface of PCL were as follows: 1.5 of COOH:EDAC ratio, 10 mM NHS concentration and, 80 mg/ml gelatin. The result shows that gelatin coated PCL microcarrier promote more and rapid cell adhesion with density of as compared to raw PCL microcarrier ( and UV/O3 treated PCL microcarrier ( . Therefore, immobilization of gelatin with optimized parameters onto PCL microcarrier improved biocompatibility of PCL microcarrier. Penerbit UTM Press 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/63284/1/Statistical%20optimization%20of%20gelatin%20immobilisation%20on%20modified%20surface%20PCL%20microcarrier%20.pdf Samsudin, Nurhusna and Hashim, Yumi Zuhanis Has-Yun and Arifin, Mohd Azmir and Mel, Maizirwan and Mohd. Salleh, Hamzah and Nordin, Norshariza and Sopyan, Iis and Jimat, Dzun Noraini (2017) Statistical optimization of gelatin immobilisation on modified surface PCL microcarrier to improve PCL microcarrier compatibility. Jurnal Teknologi, 79 (6). 167 - 175. ISSN 0127–9696; ESSN: 2180–3722 https://jurnalteknologi.utm.my/index.php/jurnalteknologi/article/view/10417 |
| spellingShingle | Samsudin, Nurhusna Hashim, Yumi Zuhanis Has-Yun Arifin, Mohd Azmir Mel, Maizirwan Mohd. Salleh, Hamzah Nordin, Norshariza Sopyan, Iis Jimat, Dzun Noraini Statistical optimization of gelatin immobilisation on modified surface PCL microcarrier to improve PCL microcarrier compatibility |
| title | Statistical optimization of gelatin immobilisation on modified surface PCL microcarrier to improve PCL microcarrier compatibility |
| title_full | Statistical optimization of gelatin immobilisation on modified surface PCL microcarrier to improve PCL microcarrier compatibility |
| title_fullStr | Statistical optimization of gelatin immobilisation on modified surface PCL microcarrier to improve PCL microcarrier compatibility |
| title_full_unstemmed | Statistical optimization of gelatin immobilisation on modified surface PCL microcarrier to improve PCL microcarrier compatibility |
| title_short | Statistical optimization of gelatin immobilisation on modified surface PCL microcarrier to improve PCL microcarrier compatibility |
| title_sort | statistical optimization of gelatin immobilisation on modified surface pcl microcarrier to improve pcl microcarrier compatibility |
| url | http://psasir.upm.edu.my/id/eprint/63284/ http://psasir.upm.edu.my/id/eprint/63284/ http://psasir.upm.edu.my/id/eprint/63284/1/Statistical%20optimization%20of%20gelatin%20immobilisation%20on%20modified%20surface%20PCL%20microcarrier%20.pdf |