Saffron extract and crocin reduced biomarkers associated with obesity in rats fed a high-fat diet
Introduction: This study aimed to investigate the effect of saffron extract and crocin on blood biomarkers associated with obesity using the rat model. Methods: Obesity was induced by feeding a high-fat diet to 42 male Sprague-Dawley rats for 12 weeks, after which they were equally distributed into...
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| Format: | Article |
| Language: | English |
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Nutrition Society of Malaysia
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/63170/ http://psasir.upm.edu.my/id/eprint/63170/1/Saffron%20extract%20and%20crocin%20reduced%20biomarkers%20associated%20with%20obesity%20in%20rats%20fed%20a%20.pdf |
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| author | Mashmoul, Maryam Azlan, Azrina Mohtarrudin, Norhafizah Mohd Yusof, Barakatun Nisak Khaza’ai, Huzwah |
| author_facet | Mashmoul, Maryam Azlan, Azrina Mohtarrudin, Norhafizah Mohd Yusof, Barakatun Nisak Khaza’ai, Huzwah |
| author_sort | Mashmoul, Maryam |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Introduction: This study aimed to investigate the effect of saffron extract and crocin on blood biomarkers associated with obesity using the rat model. Methods: Obesity was induced by feeding a high-fat diet to 42 male Sprague-Dawley rats for 12 weeks, after which they were equally distributed into seven groups. Three groups served as controls namely, normal diet (ND), high-fat diet (HFD), and high-fat diet plus orlistat (HFD + ORL), while the remaining four treatment groups consisted of HFD added low or high dose (40 and 80 mg/kg/day) of either saffron extract or crocin in the food. At the end of 8 weeks, blood samples were collected by cardiac puncture for biochemical analysis. Results: Obese rats treated with a high dose of saffron extract and crocin showed significantly lower plasma glucose levels (5.26 and 5.67 mmol/L respectively) than the HFD rats (6.92 mmol/L). Saffron extract and crocin at a high dose showed significantly lower levels of plasma insulin (3.97 and 3.88 ng/mL respctively) compared to HFD control (5.41 ng/mL). Adiponectin levels significantly increased in obese rats fed saffron extract and crocin at high doses (7.44 and 7.92 μg/mL respectively) compared to HFD control (5.34 μg/mL). Ghrelin level significantly increased from 419.10 to 284.10 pg/mL,while leptin level significantly decreased from 8.08 to 5.68 ng/mL for the high dose crocin groups compared to HFD control. No significant differences in plasma serotonin levels were found among the groups. Conclusion: Saffron extract and crocin show potential in reducing blood biomarkers associated with obesity as well as anti-inflammatory and regulatory potential of adipocytokines in an animal model. |
| first_indexed | 2025-11-15T11:14:29Z |
| format | Article |
| id | upm-63170 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:14:29Z |
| publishDate | 2017 |
| publisher | Nutrition Society of Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-631702018-08-20T01:22:27Z http://psasir.upm.edu.my/id/eprint/63170/ Saffron extract and crocin reduced biomarkers associated with obesity in rats fed a high-fat diet Mashmoul, Maryam Azlan, Azrina Mohtarrudin, Norhafizah Mohd Yusof, Barakatun Nisak Khaza’ai, Huzwah Introduction: This study aimed to investigate the effect of saffron extract and crocin on blood biomarkers associated with obesity using the rat model. Methods: Obesity was induced by feeding a high-fat diet to 42 male Sprague-Dawley rats for 12 weeks, after which they were equally distributed into seven groups. Three groups served as controls namely, normal diet (ND), high-fat diet (HFD), and high-fat diet plus orlistat (HFD + ORL), while the remaining four treatment groups consisted of HFD added low or high dose (40 and 80 mg/kg/day) of either saffron extract or crocin in the food. At the end of 8 weeks, blood samples were collected by cardiac puncture for biochemical analysis. Results: Obese rats treated with a high dose of saffron extract and crocin showed significantly lower plasma glucose levels (5.26 and 5.67 mmol/L respectively) than the HFD rats (6.92 mmol/L). Saffron extract and crocin at a high dose showed significantly lower levels of plasma insulin (3.97 and 3.88 ng/mL respctively) compared to HFD control (5.41 ng/mL). Adiponectin levels significantly increased in obese rats fed saffron extract and crocin at high doses (7.44 and 7.92 μg/mL respectively) compared to HFD control (5.34 μg/mL). Ghrelin level significantly increased from 419.10 to 284.10 pg/mL,while leptin level significantly decreased from 8.08 to 5.68 ng/mL for the high dose crocin groups compared to HFD control. No significant differences in plasma serotonin levels were found among the groups. Conclusion: Saffron extract and crocin show potential in reducing blood biomarkers associated with obesity as well as anti-inflammatory and regulatory potential of adipocytokines in an animal model. Nutrition Society of Malaysia 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/63170/1/Saffron%20extract%20and%20crocin%20reduced%20biomarkers%20associated%20with%20obesity%20in%20rats%20fed%20a%20.pdf Mashmoul, Maryam and Azlan, Azrina and Mohtarrudin, Norhafizah and Mohd Yusof, Barakatun Nisak and Khaza’ai, Huzwah (2017) Saffron extract and crocin reduced biomarkers associated with obesity in rats fed a high-fat diet. Malaysian Journal of Nutrition, 23 (1). 117 - 127. ISSN 1394-035X |
| spellingShingle | Mashmoul, Maryam Azlan, Azrina Mohtarrudin, Norhafizah Mohd Yusof, Barakatun Nisak Khaza’ai, Huzwah Saffron extract and crocin reduced biomarkers associated with obesity in rats fed a high-fat diet |
| title | Saffron extract and crocin reduced biomarkers associated with obesity in rats fed a high-fat diet |
| title_full | Saffron extract and crocin reduced biomarkers associated with obesity in rats fed a high-fat diet |
| title_fullStr | Saffron extract and crocin reduced biomarkers associated with obesity in rats fed a high-fat diet |
| title_full_unstemmed | Saffron extract and crocin reduced biomarkers associated with obesity in rats fed a high-fat diet |
| title_short | Saffron extract and crocin reduced biomarkers associated with obesity in rats fed a high-fat diet |
| title_sort | saffron extract and crocin reduced biomarkers associated with obesity in rats fed a high-fat diet |
| url | http://psasir.upm.edu.my/id/eprint/63170/ http://psasir.upm.edu.my/id/eprint/63170/1/Saffron%20extract%20and%20crocin%20reduced%20biomarkers%20associated%20with%20obesity%20in%20rats%20fed%20a%20.pdf |