Recovery and partial purification of thermophilic β-xylosidase derived from recombinant Bacillus megaterium MS941 by aqueous two-phase system
A polymer–salt-based aqueous two-phase system (ATPS) was developed for the effective extraction and purification of extracellular β-xylosidase from the fermentation broth of recombinant Bacillus megaterium MS941. The effect of molecular weight (MW) of polyethylene glycol (PEG), tie-line length (TLL)...
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| Format: | Article |
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Taylor & Francis
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/62923/ |
| _version_ | 1848854691312566272 |
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| author | Hadi, Sofijan Tan, Joo Shun Abbasiliasi, Sahar Mustafa, Shuhaimi Puspaningsih, Nyoman Tri Kadkhodaei, Saeid Ariff, Arbakariya |
| author_facet | Hadi, Sofijan Tan, Joo Shun Abbasiliasi, Sahar Mustafa, Shuhaimi Puspaningsih, Nyoman Tri Kadkhodaei, Saeid Ariff, Arbakariya |
| author_sort | Hadi, Sofijan |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | A polymer–salt-based aqueous two-phase system (ATPS) was developed for the effective extraction and purification of extracellular β-xylosidase from the fermentation broth of recombinant Bacillus megaterium MS941. The effect of molecular weight (MW) of polyethylene glycol (PEG), tie-line length (TLL), volume ratio (VR), crude loading and pH on the recovery performance was evaluated. Under the optimal extraction conditions, β-xylosidase was successfully purified up to 23-fold with a recovery yield of 99% in the bottom salt-rich phase at PEG 4,000/potassium phosphate ATPS comprising TLL of 41.8, VR of 2.3, crude loading (CL) of 30% (w/w) at pH 6. |
| first_indexed | 2025-11-15T11:13:53Z |
| format | Article |
| id | upm-62923 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T11:13:53Z |
| publishDate | 2017 |
| publisher | Taylor & Francis |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-629232022-11-25T01:39:04Z http://psasir.upm.edu.my/id/eprint/62923/ Recovery and partial purification of thermophilic β-xylosidase derived from recombinant Bacillus megaterium MS941 by aqueous two-phase system Hadi, Sofijan Tan, Joo Shun Abbasiliasi, Sahar Mustafa, Shuhaimi Puspaningsih, Nyoman Tri Kadkhodaei, Saeid Ariff, Arbakariya A polymer–salt-based aqueous two-phase system (ATPS) was developed for the effective extraction and purification of extracellular β-xylosidase from the fermentation broth of recombinant Bacillus megaterium MS941. The effect of molecular weight (MW) of polyethylene glycol (PEG), tie-line length (TLL), volume ratio (VR), crude loading and pH on the recovery performance was evaluated. Under the optimal extraction conditions, β-xylosidase was successfully purified up to 23-fold with a recovery yield of 99% in the bottom salt-rich phase at PEG 4,000/potassium phosphate ATPS comprising TLL of 41.8, VR of 2.3, crude loading (CL) of 30% (w/w) at pH 6. Taylor & Francis 2017-01 Article PeerReviewed Hadi, Sofijan and Tan, Joo Shun and Abbasiliasi, Sahar and Mustafa, Shuhaimi and Puspaningsih, Nyoman Tri and Kadkhodaei, Saeid and Ariff, Arbakariya (2017) Recovery and partial purification of thermophilic β-xylosidase derived from recombinant Bacillus megaterium MS941 by aqueous two-phase system. Separation Science and Technology, 52 (5). 834 - 842. ISSN 0149-6395; ESSN: 1520-5754 https://www.tandfonline.com/doi/full/10.1080/01496395.2016.1268159 10.1080/01496395.2016.1268159 |
| spellingShingle | Hadi, Sofijan Tan, Joo Shun Abbasiliasi, Sahar Mustafa, Shuhaimi Puspaningsih, Nyoman Tri Kadkhodaei, Saeid Ariff, Arbakariya Recovery and partial purification of thermophilic β-xylosidase derived from recombinant Bacillus megaterium MS941 by aqueous two-phase system |
| title | Recovery and partial purification of thermophilic β-xylosidase derived from recombinant Bacillus megaterium MS941 by aqueous two-phase system |
| title_full | Recovery and partial purification of thermophilic β-xylosidase derived from recombinant Bacillus megaterium MS941 by aqueous two-phase system |
| title_fullStr | Recovery and partial purification of thermophilic β-xylosidase derived from recombinant Bacillus megaterium MS941 by aqueous two-phase system |
| title_full_unstemmed | Recovery and partial purification of thermophilic β-xylosidase derived from recombinant Bacillus megaterium MS941 by aqueous two-phase system |
| title_short | Recovery and partial purification of thermophilic β-xylosidase derived from recombinant Bacillus megaterium MS941 by aqueous two-phase system |
| title_sort | recovery and partial purification of thermophilic β-xylosidase derived from recombinant bacillus megaterium ms941 by aqueous two-phase system |
| url | http://psasir.upm.edu.my/id/eprint/62923/ http://psasir.upm.edu.my/id/eprint/62923/ http://psasir.upm.edu.my/id/eprint/62923/ |