Purification and immunogenicity of hemagglutinin from highly pathogenic avian influenza virus H5N1 expressed in Nicotiana benthamiana
Highly pathogenic avian influenza (HPAI) H5N1 is an ongoing global health concern due to its severe sporadic outbreaks in Asia, Africa and Europe, which poses a potential pandemic threat. The development of safe and cost-effective vaccine candidates for HPAI is considered the best strategy for manag...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
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Taylor & Francis
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/62893/ |
| _version_ | 1848854686199709696 |
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| author | Pua, Teen-Lee Chan, Xiao Ying Loh, Hwei-San Omar, Abdul Rahman Yusibov, Vidadi Musiychuk, Konstantin Hall, Alexandra C. Coffin, Megan V. Shoji, Yoko Chichester, Jessica A. Bi, Hong Streatfield, Stephen J. |
| author_facet | Pua, Teen-Lee Chan, Xiao Ying Loh, Hwei-San Omar, Abdul Rahman Yusibov, Vidadi Musiychuk, Konstantin Hall, Alexandra C. Coffin, Megan V. Shoji, Yoko Chichester, Jessica A. Bi, Hong Streatfield, Stephen J. |
| author_sort | Pua, Teen-Lee |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Highly pathogenic avian influenza (HPAI) H5N1 is an ongoing global health concern due to its severe sporadic outbreaks in Asia, Africa and Europe, which poses a potential pandemic threat. The development of safe and cost-effective vaccine candidates for HPAI is considered the best strategy for managing the disease and addressing the pandemic preparedness. The most potential vaccine candidate is the antigenic determinant of influenza A virus, hemagglutinin (HA). The present research was aimed at developing optimized expression in Nicotiana benthamiana and protein purification process for HA from the Malaysian isolate of H5N1 as a vaccine antigen for HPAI H5N1. Expression of HA from the Malaysian isolate of HPAI in N. benthamiana was confirmed, and more soluble protein was expressed as truncated HA, the HA1 domain over the entire ectodomain of HA. Two different purification processes were evaluated for efficiency in terms of purity and yield. Due to the reduced yield, protein degradation and length of the 3-column purification process, the 2-column method was chosen for target purification. Purified HA1 was found immunogenic in mice inducing H5 HA-specific IgG and a hemagglutination inhibition antibody. This paper offers an alternative production system of a vaccine candidate against a locally circulating HPAI, which has a regional significance. |
| first_indexed | 2025-11-15T11:13:48Z |
| format | Article |
| id | upm-62893 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T11:13:48Z |
| publishDate | 2017 |
| publisher | Taylor & Francis |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-628932022-11-24T03:01:50Z http://psasir.upm.edu.my/id/eprint/62893/ Purification and immunogenicity of hemagglutinin from highly pathogenic avian influenza virus H5N1 expressed in Nicotiana benthamiana Pua, Teen-Lee Chan, Xiao Ying Loh, Hwei-San Omar, Abdul Rahman Yusibov, Vidadi Musiychuk, Konstantin Hall, Alexandra C. Coffin, Megan V. Shoji, Yoko Chichester, Jessica A. Bi, Hong Streatfield, Stephen J. Highly pathogenic avian influenza (HPAI) H5N1 is an ongoing global health concern due to its severe sporadic outbreaks in Asia, Africa and Europe, which poses a potential pandemic threat. The development of safe and cost-effective vaccine candidates for HPAI is considered the best strategy for managing the disease and addressing the pandemic preparedness. The most potential vaccine candidate is the antigenic determinant of influenza A virus, hemagglutinin (HA). The present research was aimed at developing optimized expression in Nicotiana benthamiana and protein purification process for HA from the Malaysian isolate of H5N1 as a vaccine antigen for HPAI H5N1. Expression of HA from the Malaysian isolate of HPAI in N. benthamiana was confirmed, and more soluble protein was expressed as truncated HA, the HA1 domain over the entire ectodomain of HA. Two different purification processes were evaluated for efficiency in terms of purity and yield. Due to the reduced yield, protein degradation and length of the 3-column purification process, the 2-column method was chosen for target purification. Purified HA1 was found immunogenic in mice inducing H5 HA-specific IgG and a hemagglutination inhibition antibody. This paper offers an alternative production system of a vaccine candidate against a locally circulating HPAI, which has a regional significance. Taylor & Francis 2017-01 Article PeerReviewed Pua, Teen-Lee and Chan, Xiao Ying and Loh, Hwei-San and Omar, Abdul Rahman and Yusibov, Vidadi and Musiychuk, Konstantin and Hall, Alexandra C. and Coffin, Megan V. and Shoji, Yoko and Chichester, Jessica A. and Bi, Hong and Streatfield, Stephen J. (2017) Purification and immunogenicity of hemagglutinin from highly pathogenic avian influenza virus H5N1 expressed in Nicotiana benthamiana. Human Vaccines & Immunotherapeutics, 13 (2). pp. 306-313. ISSN 2164-5515; ESSN: 2164-554X https://www.tandfonline.com/doi/full/10.1080/21645515.2017.1264783 10.1080/21645515.2017.1264783 |
| spellingShingle | Pua, Teen-Lee Chan, Xiao Ying Loh, Hwei-San Omar, Abdul Rahman Yusibov, Vidadi Musiychuk, Konstantin Hall, Alexandra C. Coffin, Megan V. Shoji, Yoko Chichester, Jessica A. Bi, Hong Streatfield, Stephen J. Purification and immunogenicity of hemagglutinin from highly pathogenic avian influenza virus H5N1 expressed in Nicotiana benthamiana |
| title | Purification and immunogenicity of hemagglutinin from highly pathogenic avian influenza virus H5N1 expressed in Nicotiana benthamiana |
| title_full | Purification and immunogenicity of hemagglutinin from highly pathogenic avian influenza virus H5N1 expressed in Nicotiana benthamiana |
| title_fullStr | Purification and immunogenicity of hemagglutinin from highly pathogenic avian influenza virus H5N1 expressed in Nicotiana benthamiana |
| title_full_unstemmed | Purification and immunogenicity of hemagglutinin from highly pathogenic avian influenza virus H5N1 expressed in Nicotiana benthamiana |
| title_short | Purification and immunogenicity of hemagglutinin from highly pathogenic avian influenza virus H5N1 expressed in Nicotiana benthamiana |
| title_sort | purification and immunogenicity of hemagglutinin from highly pathogenic avian influenza virus h5n1 expressed in nicotiana benthamiana |
| url | http://psasir.upm.edu.my/id/eprint/62893/ http://psasir.upm.edu.my/id/eprint/62893/ http://psasir.upm.edu.my/id/eprint/62893/ |