Proteomic analysis of synovial fluid obtained from a dog diagnosed with idiopathic immune-mediated polyarthritis
This case study is to report the proteins detected by proteomic analysis of synovial fluid from a dog diagnosed with idiopathic immune-mediated polyarthritis, and to compare it with healthy dogs. Synovial fluid was collected via arthrocentesis from a dog diagnosed with immune-mediated polyarthritis....
| Main Authors: | , , , , , |
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| Format: | Article |
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Elsevier
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/62880/ |
| _version_ | 1848854683443003392 |
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| author | Tan, Wei Miao Lau, Seng Fong Md Ajat, Mohd Mokrish Mansor, Rozaihan Megat Abd Rani, Puteri Azaziah Rahmad, Norasfaliza |
| author_facet | Tan, Wei Miao Lau, Seng Fong Md Ajat, Mohd Mokrish Mansor, Rozaihan Megat Abd Rani, Puteri Azaziah Rahmad, Norasfaliza |
| author_sort | Tan, Wei Miao |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | This case study is to report the proteins detected by proteomic analysis of synovial fluid from a dog diagnosed with idiopathic immune-mediated polyarthritis, and to compare it with healthy dogs. Synovial fluid was collected via arthrocentesis from a dog diagnosed with immune-mediated polyarthritis. Protein precipitation was performed on the synovial fluid, followed by isoelectric focusing and 2-dimensional gel electrophoresis. The spots on the 2-dimensional gels were analyzed using MALDI-TOF/MS. The results were then analyzed against the MASCOT database. The results from the proteomic analysis revealed an abundance of several types of immunoglobulins together with the presence of complement C4b-binding protein alpha chain. Actin and keratin were also among the proteins detected. Proteomic studies, facilitate a better understanding of the different levels of proteins expressed during disease activity. Potential disease biomarkers can aid in the diagnosis of disease, as well as help in monitoring treatment efficacy and providing prognosis for the patient. |
| first_indexed | 2025-11-15T11:13:46Z |
| format | Article |
| id | upm-62880 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T11:13:46Z |
| publishDate | 2017 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-628802022-11-23T03:14:37Z http://psasir.upm.edu.my/id/eprint/62880/ Proteomic analysis of synovial fluid obtained from a dog diagnosed with idiopathic immune-mediated polyarthritis Tan, Wei Miao Lau, Seng Fong Md Ajat, Mohd Mokrish Mansor, Rozaihan Megat Abd Rani, Puteri Azaziah Rahmad, Norasfaliza This case study is to report the proteins detected by proteomic analysis of synovial fluid from a dog diagnosed with idiopathic immune-mediated polyarthritis, and to compare it with healthy dogs. Synovial fluid was collected via arthrocentesis from a dog diagnosed with immune-mediated polyarthritis. Protein precipitation was performed on the synovial fluid, followed by isoelectric focusing and 2-dimensional gel electrophoresis. The spots on the 2-dimensional gels were analyzed using MALDI-TOF/MS. The results were then analyzed against the MASCOT database. The results from the proteomic analysis revealed an abundance of several types of immunoglobulins together with the presence of complement C4b-binding protein alpha chain. Actin and keratin were also among the proteins detected. Proteomic studies, facilitate a better understanding of the different levels of proteins expressed during disease activity. Potential disease biomarkers can aid in the diagnosis of disease, as well as help in monitoring treatment efficacy and providing prognosis for the patient. Elsevier 2017-03 Article PeerReviewed Tan, Wei Miao and Lau, Seng Fong and Md Ajat, Mohd Mokrish and Mansor, Rozaihan and Megat Abd Rani, Puteri Azaziah and Rahmad, Norasfaliza (2017) Proteomic analysis of synovial fluid obtained from a dog diagnosed with idiopathic immune-mediated polyarthritis. Topics in Companion Animal Medicine, 32 (1). pp. 24-27. ISSN 1938-9736; ESSN:1946-9837 https://www.sciencedirect.com/science/article/pii/S1938973617300284 10.1053/j.tcam.2017.05.002 |
| spellingShingle | Tan, Wei Miao Lau, Seng Fong Md Ajat, Mohd Mokrish Mansor, Rozaihan Megat Abd Rani, Puteri Azaziah Rahmad, Norasfaliza Proteomic analysis of synovial fluid obtained from a dog diagnosed with idiopathic immune-mediated polyarthritis |
| title | Proteomic analysis of synovial fluid obtained from a dog diagnosed with idiopathic immune-mediated polyarthritis |
| title_full | Proteomic analysis of synovial fluid obtained from a dog diagnosed with idiopathic immune-mediated polyarthritis |
| title_fullStr | Proteomic analysis of synovial fluid obtained from a dog diagnosed with idiopathic immune-mediated polyarthritis |
| title_full_unstemmed | Proteomic analysis of synovial fluid obtained from a dog diagnosed with idiopathic immune-mediated polyarthritis |
| title_short | Proteomic analysis of synovial fluid obtained from a dog diagnosed with idiopathic immune-mediated polyarthritis |
| title_sort | proteomic analysis of synovial fluid obtained from a dog diagnosed with idiopathic immune-mediated polyarthritis |
| url | http://psasir.upm.edu.my/id/eprint/62880/ http://psasir.upm.edu.my/id/eprint/62880/ http://psasir.upm.edu.my/id/eprint/62880/ |