Pro-inflammatory cytokine (IL-1β and IL-6) response in goats challenged with Corynebacterium pseudotuberculosis and its immunogen Mycolic Acids
The causative agent of Caseous Lymphadenitis (CLA) is Corynebacterium pseudotuberculosis. A chronic disease of sheep and goats which is mainly characterized by suppurative abscesses in both superficial and visceral lymph nodes. There is paucity of information on the proinflammatory cytokine (IL-1β a...
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| Format: | Article |
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Science Publications
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/62870/ |
| _version_ | 1848854681498943488 |
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| author | Abdullah, Faez Firdaus Jesse Mohammed Naji, Ahmed Odhah Mohd Lila, Mohd Azmi Saad, Mohd Zamri Haron, Abd Wahid Hambali, Idris Umar Mahmood, Zaid Khudher |
| author_facet | Abdullah, Faez Firdaus Jesse Mohammed Naji, Ahmed Odhah Mohd Lila, Mohd Azmi Saad, Mohd Zamri Haron, Abd Wahid Hambali, Idris Umar Mahmood, Zaid Khudher |
| author_sort | Abdullah, Faez Firdaus Jesse |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The causative agent of Caseous Lymphadenitis (CLA) is Corynebacterium pseudotuberculosis. A chronic disease of sheep and goats which is mainly characterized by suppurative abscesses in both superficial and visceral lymph nodes. There is paucity of information on the proinflammatory cytokine (IL-1β and IL-6) responses due to C. pseudotuberculosis and Mycolic Acid (MAs) inoculation in goats. Therefore, this study was conducted to evaluate the immunogenicity of C. pseudotuberculosis and MAs. Twelve clinically healthy crossbred Boer female goats were divided into 3 groups; A, B and C with 4 goats per group. The A group was injected with 2 mL sterile Phosphate Buffered Saline (PBS) intradermally as negative control group while B group was inoculated with 2 mL of MAs extract intradermally. Group C was inoculated with 2 mL 1×109 colony forming unit (cfu) of live C. pseudotuberculosis intradermally. Blood was collected from the jugular vein periodically for IL-1β and IL-6 analysis throughout the experimental period. The results showed significant increase (p<0.05) in IL-1β concentration in weeks two, three, four, five, six, eight, nine and ten in C. pseudotuberculosis inoculated group, whilst in MAs inoculated group, IL-1β concentration increased significantly (p<0.05) in weeks three, five, six, eight, nine and ten compared to the control. However, IL-1β showed significant decrease (p<0.05) in week twelve in both treated groups. The concentration of IL-6 was significantly increased (p<0.05) in weeks five, six, seven and eight in C. pseudotuberculosis inoculated group compared to the control, whilst MAs inoculated group indicated a significant increase (p<0.05) in weeks two, three, four, five, six and eight compared to the control. Therefore, this study concluded that MAs extracted from C. pseudotuberculosis has immunogenic properties represented by all the significant changes in concentration of IL-1 β and IL-6. |
| first_indexed | 2025-11-15T11:13:44Z |
| format | Article |
| id | upm-62870 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T11:13:44Z |
| publishDate | 2017 |
| publisher | Science Publications |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-628702022-11-23T02:02:17Z http://psasir.upm.edu.my/id/eprint/62870/ Pro-inflammatory cytokine (IL-1β and IL-6) response in goats challenged with Corynebacterium pseudotuberculosis and its immunogen Mycolic Acids Abdullah, Faez Firdaus Jesse Mohammed Naji, Ahmed Odhah Mohd Lila, Mohd Azmi Saad, Mohd Zamri Haron, Abd Wahid Hambali, Idris Umar Mahmood, Zaid Khudher The causative agent of Caseous Lymphadenitis (CLA) is Corynebacterium pseudotuberculosis. A chronic disease of sheep and goats which is mainly characterized by suppurative abscesses in both superficial and visceral lymph nodes. There is paucity of information on the proinflammatory cytokine (IL-1β and IL-6) responses due to C. pseudotuberculosis and Mycolic Acid (MAs) inoculation in goats. Therefore, this study was conducted to evaluate the immunogenicity of C. pseudotuberculosis and MAs. Twelve clinically healthy crossbred Boer female goats were divided into 3 groups; A, B and C with 4 goats per group. The A group was injected with 2 mL sterile Phosphate Buffered Saline (PBS) intradermally as negative control group while B group was inoculated with 2 mL of MAs extract intradermally. Group C was inoculated with 2 mL 1×109 colony forming unit (cfu) of live C. pseudotuberculosis intradermally. Blood was collected from the jugular vein periodically for IL-1β and IL-6 analysis throughout the experimental period. The results showed significant increase (p<0.05) in IL-1β concentration in weeks two, three, four, five, six, eight, nine and ten in C. pseudotuberculosis inoculated group, whilst in MAs inoculated group, IL-1β concentration increased significantly (p<0.05) in weeks three, five, six, eight, nine and ten compared to the control. However, IL-1β showed significant decrease (p<0.05) in week twelve in both treated groups. The concentration of IL-6 was significantly increased (p<0.05) in weeks five, six, seven and eight in C. pseudotuberculosis inoculated group compared to the control, whilst MAs inoculated group indicated a significant increase (p<0.05) in weeks two, three, four, five, six and eight compared to the control. Therefore, this study concluded that MAs extracted from C. pseudotuberculosis has immunogenic properties represented by all the significant changes in concentration of IL-1 β and IL-6. Science Publications 2017-02 Article PeerReviewed Abdullah, Faez Firdaus Jesse and Mohammed Naji, Ahmed Odhah and Mohd Lila, Mohd Azmi and Saad, Mohd Zamri and Haron, Abd Wahid and Hambali, Idris Umar and Mahmood, Zaid Khudher (2017) Pro-inflammatory cytokine (IL-1β and IL-6) response in goats challenged with Corynebacterium pseudotuberculosis and its immunogen Mycolic Acids. American Journal of Animal and Veterinary Sciences, 12 (1). 26 - 31. ISSN 1557-4555; ESSN: 1557-4563 https://thescipub.com/abstract/ajavsp.2017.26.31 10.3844/ajavsp.2017.26.31 |
| spellingShingle | Abdullah, Faez Firdaus Jesse Mohammed Naji, Ahmed Odhah Mohd Lila, Mohd Azmi Saad, Mohd Zamri Haron, Abd Wahid Hambali, Idris Umar Mahmood, Zaid Khudher Pro-inflammatory cytokine (IL-1β and IL-6) response in goats challenged with Corynebacterium pseudotuberculosis and its immunogen Mycolic Acids |
| title | Pro-inflammatory cytokine (IL-1β and IL-6) response in goats challenged with Corynebacterium pseudotuberculosis and its immunogen Mycolic Acids |
| title_full | Pro-inflammatory cytokine (IL-1β and IL-6) response in goats challenged with Corynebacterium pseudotuberculosis and its immunogen Mycolic Acids |
| title_fullStr | Pro-inflammatory cytokine (IL-1β and IL-6) response in goats challenged with Corynebacterium pseudotuberculosis and its immunogen Mycolic Acids |
| title_full_unstemmed | Pro-inflammatory cytokine (IL-1β and IL-6) response in goats challenged with Corynebacterium pseudotuberculosis and its immunogen Mycolic Acids |
| title_short | Pro-inflammatory cytokine (IL-1β and IL-6) response in goats challenged with Corynebacterium pseudotuberculosis and its immunogen Mycolic Acids |
| title_sort | pro-inflammatory cytokine (il-1β and il-6) response in goats challenged with corynebacterium pseudotuberculosis and its immunogen mycolic acids |
| url | http://psasir.upm.edu.my/id/eprint/62870/ http://psasir.upm.edu.my/id/eprint/62870/ http://psasir.upm.edu.my/id/eprint/62870/ |