Preparation and characterization of neem oil nanoemulsion formulations against Sitophilus oryzae and Tribolium castaneum adults

This study aimed to improve the efficacy of azadirachtin (Azadirachta indica. A. Juss) against two serious pest species of stored products, Sitophilus oryzae (L.) and Tribolium castaneum (Herbst), through nano-emulsion formulations. Pseudoternary phase diagrams were constructed consisting of an emul...

Full description

Bibliographic Details
Main Authors: Choupanian, Marziyeh, Omar, Dzolkifli, Basri, Mahiran, Asib, Norhayu
Format: Article
Language:English
Published: Pesticide Science Society of Japan 2017
Online Access:http://psasir.upm.edu.my/id/eprint/62826/
http://psasir.upm.edu.my/id/eprint/62826/1/Preparation%20and%20characterization%20of%20neem%20oil%20.pdf
_version_ 1848854674284740608
author Choupanian, Marziyeh
Omar, Dzolkifli
Basri, Mahiran
Asib, Norhayu
author_facet Choupanian, Marziyeh
Omar, Dzolkifli
Basri, Mahiran
Asib, Norhayu
author_sort Choupanian, Marziyeh
building UPM Institutional Repository
collection Online Access
description This study aimed to improve the efficacy of azadirachtin (Azadirachta indica. A. Juss) against two serious pest species of stored products, Sitophilus oryzae (L.) and Tribolium castaneum (Herbst), through nano-emulsion formulations. Pseudoternary phase diagrams were constructed consisting of an emulsion system of an active ingredient (neem oil), surfactant (polysorbate or alkylpolyglucoside), and water. Isotropic regions were formed in the pseudoternary phase diagrams, and four formulations were selected from the isotropic regions and characterized according to particle size, particle aging, zeta potential, stability and thermostability, surface tension, viscosity, and pH. The selected formulations showed particle sizes of 208–507 nm in diameter. The result of contact toxicity demonstrated excellent mortality of S. oryzae and T. castaneum adults, with a mortality range of 85–100% and 74–100%, respectively, at a 1% azadirachtin concentration after only 2 days of exposure. Compared to non-formulated neem oil, the nano-emulsion formulations significantly increased the mortality of the tested species.
first_indexed 2025-11-15T11:13:37Z
format Article
id upm-62826
institution Universiti Putra Malaysia
institution_category Local University
language English
last_indexed 2025-11-15T11:13:37Z
publishDate 2017
publisher Pesticide Science Society of Japan
recordtype eprints
repository_type Digital Repository
spelling upm-628262022-11-10T03:56:37Z http://psasir.upm.edu.my/id/eprint/62826/ Preparation and characterization of neem oil nanoemulsion formulations against Sitophilus oryzae and Tribolium castaneum adults Choupanian, Marziyeh Omar, Dzolkifli Basri, Mahiran Asib, Norhayu This study aimed to improve the efficacy of azadirachtin (Azadirachta indica. A. Juss) against two serious pest species of stored products, Sitophilus oryzae (L.) and Tribolium castaneum (Herbst), through nano-emulsion formulations. Pseudoternary phase diagrams were constructed consisting of an emulsion system of an active ingredient (neem oil), surfactant (polysorbate or alkylpolyglucoside), and water. Isotropic regions were formed in the pseudoternary phase diagrams, and four formulations were selected from the isotropic regions and characterized according to particle size, particle aging, zeta potential, stability and thermostability, surface tension, viscosity, and pH. The selected formulations showed particle sizes of 208–507 nm in diameter. The result of contact toxicity demonstrated excellent mortality of S. oryzae and T. castaneum adults, with a mortality range of 85–100% and 74–100%, respectively, at a 1% azadirachtin concentration after only 2 days of exposure. Compared to non-formulated neem oil, the nano-emulsion formulations significantly increased the mortality of the tested species. Pesticide Science Society of Japan 2017-11 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/62826/1/Preparation%20and%20characterization%20of%20neem%20oil%20.pdf Choupanian, Marziyeh and Omar, Dzolkifli and Basri, Mahiran and Asib, Norhayu (2017) Preparation and characterization of neem oil nanoemulsion formulations against Sitophilus oryzae and Tribolium castaneum adults. Journal of Pesticide Science, 42 (4). pp. 158-165. ISSN 1348-589X; ESSN: 1349-0923 https://www.jstage.jst.go.jp/article/jpestics/42/4/42_D17-032/_pdf/-char/en 10.1584/jpestics.D17-032
spellingShingle Choupanian, Marziyeh
Omar, Dzolkifli
Basri, Mahiran
Asib, Norhayu
Preparation and characterization of neem oil nanoemulsion formulations against Sitophilus oryzae and Tribolium castaneum adults
title Preparation and characterization of neem oil nanoemulsion formulations against Sitophilus oryzae and Tribolium castaneum adults
title_full Preparation and characterization of neem oil nanoemulsion formulations against Sitophilus oryzae and Tribolium castaneum adults
title_fullStr Preparation and characterization of neem oil nanoemulsion formulations against Sitophilus oryzae and Tribolium castaneum adults
title_full_unstemmed Preparation and characterization of neem oil nanoemulsion formulations against Sitophilus oryzae and Tribolium castaneum adults
title_short Preparation and characterization of neem oil nanoemulsion formulations against Sitophilus oryzae and Tribolium castaneum adults
title_sort preparation and characterization of neem oil nanoemulsion formulations against sitophilus oryzae and tribolium castaneum adults
url http://psasir.upm.edu.my/id/eprint/62826/
http://psasir.upm.edu.my/id/eprint/62826/
http://psasir.upm.edu.my/id/eprint/62826/
http://psasir.upm.edu.my/id/eprint/62826/1/Preparation%20and%20characterization%20of%20neem%20oil%20.pdf