Prebiotic evaluation of red seaweed (Kappaphycus alvarezii) using in vitro colon model
Red seaweed (Kappaphycus alvarezii) cultivated from Sabah (RSS) and Langkawi (RSL) were digested using in vitro mouth, gastric and duodenal model. The digested seaweed then fermented in a pH-controlled batch culture system inoculated with human faeces to mimic the distal colon. Bacterial enumeration...
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| Format: | Article |
| Language: | English |
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Taylor & Francis
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/62796/ http://psasir.upm.edu.my/id/eprint/62796/1/Prebiotic%20evaluation%20.pdf |
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| author | Bajury, Dayang Marshitah Rawi, Muhamad Hanif Sazali, Iqbal Hakim Abdullah, Aminah Sarbini, Shahrul Razid |
| author_facet | Bajury, Dayang Marshitah Rawi, Muhamad Hanif Sazali, Iqbal Hakim Abdullah, Aminah Sarbini, Shahrul Razid |
| author_sort | Bajury, Dayang Marshitah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Red seaweed (Kappaphycus alvarezii) cultivated from Sabah (RSS) and Langkawi (RSL) were digested using in vitro mouth, gastric and duodenal model. The digested seaweed then fermented in a pH-controlled batch culture system inoculated with human faeces to mimic the distal colon. Bacterial enumeration were monitored using fluorescent in situ hybridisation, and the fermentation end products, the short chain fatty acids (SCFA), were analysed using HPLC. Both RSS and RSL showed significant increase of Bifidobacterium sp.; from log10 7.96 at 0 h to log10 8.72 at 24 h, and from log10 7.96 at 0 h to log10 8.60 at 24 h, respectively, and shows no significant difference when compared to the Bifidobacterium sp. count at 24 h of inulin fermentation. Both seaweeds also showed significant increase in total SCFA production, particularly acetate and propionate. Overall, this data suggested that K. alvarezii might have the potential as a prebiotic ingredient. |
| first_indexed | 2025-11-15T11:13:31Z |
| format | Article |
| id | upm-62796 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:13:31Z |
| publishDate | 2017 |
| publisher | Taylor & Francis |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-627962022-11-09T02:35:32Z http://psasir.upm.edu.my/id/eprint/62796/ Prebiotic evaluation of red seaweed (Kappaphycus alvarezii) using in vitro colon model Bajury, Dayang Marshitah Rawi, Muhamad Hanif Sazali, Iqbal Hakim Abdullah, Aminah Sarbini, Shahrul Razid Red seaweed (Kappaphycus alvarezii) cultivated from Sabah (RSS) and Langkawi (RSL) were digested using in vitro mouth, gastric and duodenal model. The digested seaweed then fermented in a pH-controlled batch culture system inoculated with human faeces to mimic the distal colon. Bacterial enumeration were monitored using fluorescent in situ hybridisation, and the fermentation end products, the short chain fatty acids (SCFA), were analysed using HPLC. Both RSS and RSL showed significant increase of Bifidobacterium sp.; from log10 7.96 at 0 h to log10 8.72 at 24 h, and from log10 7.96 at 0 h to log10 8.60 at 24 h, respectively, and shows no significant difference when compared to the Bifidobacterium sp. count at 24 h of inulin fermentation. Both seaweeds also showed significant increase in total SCFA production, particularly acetate and propionate. Overall, this data suggested that K. alvarezii might have the potential as a prebiotic ingredient. Taylor & Francis 2017-04 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/62796/1/Prebiotic%20evaluation%20.pdf Bajury, Dayang Marshitah and Rawi, Muhamad Hanif and Sazali, Iqbal Hakim and Abdullah, Aminah and Sarbini, Shahrul Razid (2017) Prebiotic evaluation of red seaweed (Kappaphycus alvarezii) using in vitro colon model. International Journal of Food Sciences and Nutrition, 68 (7). 821 - 828. ISSN 0963-7486; ESSN: 1465-3478 https://www.tandfonline.com/doi/full/10.1080/09637486.2017.1309522 10.1080/09637486.2017.1309522 |
| spellingShingle | Bajury, Dayang Marshitah Rawi, Muhamad Hanif Sazali, Iqbal Hakim Abdullah, Aminah Sarbini, Shahrul Razid Prebiotic evaluation of red seaweed (Kappaphycus alvarezii) using in vitro colon model |
| title | Prebiotic evaluation of red seaweed (Kappaphycus alvarezii) using in vitro colon model |
| title_full | Prebiotic evaluation of red seaweed (Kappaphycus alvarezii) using in vitro colon model |
| title_fullStr | Prebiotic evaluation of red seaweed (Kappaphycus alvarezii) using in vitro colon model |
| title_full_unstemmed | Prebiotic evaluation of red seaweed (Kappaphycus alvarezii) using in vitro colon model |
| title_short | Prebiotic evaluation of red seaweed (Kappaphycus alvarezii) using in vitro colon model |
| title_sort | prebiotic evaluation of red seaweed (kappaphycus alvarezii) using in vitro colon model |
| url | http://psasir.upm.edu.my/id/eprint/62796/ http://psasir.upm.edu.my/id/eprint/62796/ http://psasir.upm.edu.my/id/eprint/62796/ http://psasir.upm.edu.my/id/eprint/62796/1/Prebiotic%20evaluation%20.pdf |