Potential of Trichoderma harzianum as cellulose biodegrade in biocomposting of paddy straw

The present study was conducted to screen the significant cellulase (cellulolytic enzyme) produced by locally isolated fungi, Trichoderma harzianum using submerged culture system. Screening of the cellulase was conducted using carboxylmethylcellulase (CMC) plate assay to assist in determining of th...

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Main Authors: Yaacob, Siti Zalina, Abdullah, Norhafizah, Abdullah, Luqman Chuah
Format: Article
Language:English
Published: Akademia Baru 2017
Online Access:http://psasir.upm.edu.my/id/eprint/62790/
http://psasir.upm.edu.my/id/eprint/62790/1/Potential%20of%20Trichoderma%20.pdf
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author Yaacob, Siti Zalina
Abdullah, Norhafizah
Abdullah, Luqman Chuah
author_facet Yaacob, Siti Zalina
Abdullah, Norhafizah
Abdullah, Luqman Chuah
author_sort Yaacob, Siti Zalina
building UPM Institutional Repository
collection Online Access
description The present study was conducted to screen the significant cellulase (cellulolytic enzyme) produced by locally isolated fungi, Trichoderma harzianum using submerged culture system. Screening of the cellulase was conducted using carboxylmethylcellulase (CMC) plate assay to assist in determining of the microorganism potential as biodegrader. The highest diameter of inhibition zone around the T.harzianum colony which was grown on CMC agar was recorded at 1.9cm from a culture that used CMC supplemented with instant yeast. The culture with the best morphology was observed after 6 days incubation at temperature of 28°C and agitation speed of 150rpm. The biocomposting study on paddy straw showed that the waste is suitable for biocompost production with the lowest C:N ratio of 17.5 after 90 days of composting period.
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spelling upm-627902022-11-09T01:56:16Z http://psasir.upm.edu.my/id/eprint/62790/ Potential of Trichoderma harzianum as cellulose biodegrade in biocomposting of paddy straw Yaacob, Siti Zalina Abdullah, Norhafizah Abdullah, Luqman Chuah The present study was conducted to screen the significant cellulase (cellulolytic enzyme) produced by locally isolated fungi, Trichoderma harzianum using submerged culture system. Screening of the cellulase was conducted using carboxylmethylcellulase (CMC) plate assay to assist in determining of the microorganism potential as biodegrader. The highest diameter of inhibition zone around the T.harzianum colony which was grown on CMC agar was recorded at 1.9cm from a culture that used CMC supplemented with instant yeast. The culture with the best morphology was observed after 6 days incubation at temperature of 28°C and agitation speed of 150rpm. The biocomposting study on paddy straw showed that the waste is suitable for biocompost production with the lowest C:N ratio of 17.5 after 90 days of composting period. Akademia Baru 2017-11 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/62790/1/Potential%20of%20Trichoderma%20.pdf Yaacob, Siti Zalina and Abdullah, Norhafizah and Abdullah, Luqman Chuah (2017) Potential of Trichoderma harzianum as cellulose biodegrade in biocomposting of paddy straw. Journal of Advanced Research in Materials Science, 39 (1). pp. 8-13. ISSN 2289-7992 https://www.akademiabaru.com/doc/ARMSV39_N1_P8_13.pdf
spellingShingle Yaacob, Siti Zalina
Abdullah, Norhafizah
Abdullah, Luqman Chuah
Potential of Trichoderma harzianum as cellulose biodegrade in biocomposting of paddy straw
title Potential of Trichoderma harzianum as cellulose biodegrade in biocomposting of paddy straw
title_full Potential of Trichoderma harzianum as cellulose biodegrade in biocomposting of paddy straw
title_fullStr Potential of Trichoderma harzianum as cellulose biodegrade in biocomposting of paddy straw
title_full_unstemmed Potential of Trichoderma harzianum as cellulose biodegrade in biocomposting of paddy straw
title_short Potential of Trichoderma harzianum as cellulose biodegrade in biocomposting of paddy straw
title_sort potential of trichoderma harzianum as cellulose biodegrade in biocomposting of paddy straw
url http://psasir.upm.edu.my/id/eprint/62790/
http://psasir.upm.edu.my/id/eprint/62790/
http://psasir.upm.edu.my/id/eprint/62790/1/Potential%20of%20Trichoderma%20.pdf