Phylogenetic characterization of genes encoding for viral envelope glycoprotein (ORF5) and nucleocapsid protein (ORF7) of porcine reproductive & respiratory syndrome virus found in Malaysia in 2013 and 2014
Background: Porcine reproductive and respiratory syndrome (PRRS) is one of the most expensive diseases of modern swine production & results in annual economic losses and cost the industry over 600 million USD in U.S. alone and billions of dollars worldwide. Two atypical PRRS cases were observe...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BioMed Central
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/62741/ http://psasir.upm.edu.my/id/eprint/62741/1/Phylogenetic%20characterization.pdf |
| _version_ | 1848854654850433024 |
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| author | King, Seetha Jaganathan Ooi, Peck Toung Phang, Lai Yee Allaudin, Zeenathul Nazariah Loh, Wei Hoong Tee, Chiou Yan How, Shiao Pau Yip, Lai Siong Choo, Pow Yoon Lim, Ban Keong |
| author_facet | King, Seetha Jaganathan Ooi, Peck Toung Phang, Lai Yee Allaudin, Zeenathul Nazariah Loh, Wei Hoong Tee, Chiou Yan How, Shiao Pau Yip, Lai Siong Choo, Pow Yoon Lim, Ban Keong |
| author_sort | King, Seetha Jaganathan |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Background: Porcine reproductive and respiratory syndrome (PRRS) is one of the most expensive diseases of
modern swine production & results in annual economic losses and cost the industry over 600 million USD in U.S.
alone and billions of dollars worldwide. Two atypical PRRS cases were observed in 2013 and 2014 characterized by
late-term abortion, fever and sudden increase in sow mortality which persisted for a prolonged period of time.
Methods: Lungs, lymph nodes and other samples were collected for disease investigation. Sequencing of the viral
envelope glycoprotein (ORF5) and nucleocapsid protein (ORF7) of PRRSV was done using the BigDye Terminator v3.
1 cycle sequencing kit chemistry. The phylogenetic tree was constructed by using the Maximum Likelihood
method, generated by Mega 6.06®.
Results: Analysis of the ORF5 and ORF7 showed high degree of sequence homology to PRRSV parent vaccine
strain VR-2332, RespPRRSV and other mutant/chimeric virus strains. Conclusions: Our study suggests that recombination events between vaccine strains and field isolates may
contribute to PRRSV virulence in the field. |
| first_indexed | 2025-11-15T11:13:19Z |
| format | Article |
| id | upm-62741 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:13:19Z |
| publishDate | 2017 |
| publisher | BioMed Central |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-627412022-11-07T01:02:10Z http://psasir.upm.edu.my/id/eprint/62741/ Phylogenetic characterization of genes encoding for viral envelope glycoprotein (ORF5) and nucleocapsid protein (ORF7) of porcine reproductive & respiratory syndrome virus found in Malaysia in 2013 and 2014 King, Seetha Jaganathan Ooi, Peck Toung Phang, Lai Yee Allaudin, Zeenathul Nazariah Loh, Wei Hoong Tee, Chiou Yan How, Shiao Pau Yip, Lai Siong Choo, Pow Yoon Lim, Ban Keong Background: Porcine reproductive and respiratory syndrome (PRRS) is one of the most expensive diseases of modern swine production & results in annual economic losses and cost the industry over 600 million USD in U.S. alone and billions of dollars worldwide. Two atypical PRRS cases were observed in 2013 and 2014 characterized by late-term abortion, fever and sudden increase in sow mortality which persisted for a prolonged period of time. Methods: Lungs, lymph nodes and other samples were collected for disease investigation. Sequencing of the viral envelope glycoprotein (ORF5) and nucleocapsid protein (ORF7) of PRRSV was done using the BigDye Terminator v3. 1 cycle sequencing kit chemistry. The phylogenetic tree was constructed by using the Maximum Likelihood method, generated by Mega 6.06®. Results: Analysis of the ORF5 and ORF7 showed high degree of sequence homology to PRRSV parent vaccine strain VR-2332, RespPRRSV and other mutant/chimeric virus strains. Conclusions: Our study suggests that recombination events between vaccine strains and field isolates may contribute to PRRSV virulence in the field. BioMed Central 2017-01 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/62741/1/Phylogenetic%20characterization.pdf King, Seetha Jaganathan and Ooi, Peck Toung and Phang, Lai Yee and Allaudin, Zeenathul Nazariah and Loh, Wei Hoong and Tee, Chiou Yan and How, Shiao Pau and Yip, Lai Siong and Choo, Pow Yoon and Lim, Ban Keong (2017) Phylogenetic characterization of genes encoding for viral envelope glycoprotein (ORF5) and nucleocapsid protein (ORF7) of porcine reproductive & respiratory syndrome virus found in Malaysia in 2013 and 2014. BMC Veterinary Research, 13 (3). pp. 1-14. ISSN 1746-6148 https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-016-0933-z 10.1186/s12917-016-0933-z |
| spellingShingle | King, Seetha Jaganathan Ooi, Peck Toung Phang, Lai Yee Allaudin, Zeenathul Nazariah Loh, Wei Hoong Tee, Chiou Yan How, Shiao Pau Yip, Lai Siong Choo, Pow Yoon Lim, Ban Keong Phylogenetic characterization of genes encoding for viral envelope glycoprotein (ORF5) and nucleocapsid protein (ORF7) of porcine reproductive & respiratory syndrome virus found in Malaysia in 2013 and 2014 |
| title | Phylogenetic characterization of genes encoding for viral envelope glycoprotein (ORF5) and nucleocapsid protein (ORF7) of porcine reproductive & respiratory syndrome virus found in Malaysia in 2013 and 2014 |
| title_full | Phylogenetic characterization of genes encoding for viral envelope glycoprotein (ORF5) and nucleocapsid protein (ORF7) of porcine reproductive & respiratory syndrome virus found in Malaysia in 2013 and 2014 |
| title_fullStr | Phylogenetic characterization of genes encoding for viral envelope glycoprotein (ORF5) and nucleocapsid protein (ORF7) of porcine reproductive & respiratory syndrome virus found in Malaysia in 2013 and 2014 |
| title_full_unstemmed | Phylogenetic characterization of genes encoding for viral envelope glycoprotein (ORF5) and nucleocapsid protein (ORF7) of porcine reproductive & respiratory syndrome virus found in Malaysia in 2013 and 2014 |
| title_short | Phylogenetic characterization of genes encoding for viral envelope glycoprotein (ORF5) and nucleocapsid protein (ORF7) of porcine reproductive & respiratory syndrome virus found in Malaysia in 2013 and 2014 |
| title_sort | phylogenetic characterization of genes encoding for viral envelope glycoprotein (orf5) and nucleocapsid protein (orf7) of porcine reproductive & respiratory syndrome virus found in malaysia in 2013 and 2014 |
| url | http://psasir.upm.edu.my/id/eprint/62741/ http://psasir.upm.edu.my/id/eprint/62741/ http://psasir.upm.edu.my/id/eprint/62741/ http://psasir.upm.edu.my/id/eprint/62741/1/Phylogenetic%20characterization.pdf |