Optimization of metallo-keratinase production by Pseudomonas sp. LM19 as a potential enzyme for feather waste conversion
Locally isolated bacterium Pseudomonas sp. LM19, a metallo-keratinase producer was used to hydrolyze the highly rigid keratin recalcitrant in this study. The production of crude keratinase by Pseudomonas sp. LM19 is influenced by both physical and nutritional parameters. The highest keratinase activ...
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| Format: | Article |
| Language: | English |
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Informa Healthcare
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/62615/ http://psasir.upm.edu.my/id/eprint/62615/1/KERATIN.pdf |
| _version_ | 1848854642971115520 |
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| author | Mohamad, Nurliyana Phang, Lai Yee Abd Aziz, Suraini |
| author_facet | Mohamad, Nurliyana Phang, Lai Yee Abd Aziz, Suraini |
| author_sort | Mohamad, Nurliyana |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Locally isolated bacterium Pseudomonas sp. LM19, a metallo-keratinase producer was used to hydrolyze the highly rigid keratin recalcitrant in this study. The production of crude keratinase by Pseudomonas sp. LM19 is influenced by both physical and nutritional parameters. The highest keratinase activity of 127 U/ml (2.15-fold) was observed in feather meal medium supplemented with fructose and peptone at a C/N ratio of 40. The optimum pH and temperature for keratinase production were found to be pH 8 and 30 °C, using 1% (w/v) feather as substrate. The degradation rate of the feathers was increased 2.4-fold at optimized physical and nutritional conditions. Feather degradation by Pseudomonas sp. LM19 led to the production of free amino acids such as arginine, glycine, leucine, and serine. The information on the production of keratinase by Pseudomonas sp. LM19 obtained from this study warrants further research for possible commercial application. |
| first_indexed | 2025-11-15T11:13:07Z |
| format | Article |
| id | upm-62615 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:13:07Z |
| publishDate | 2017 |
| publisher | Informa Healthcare |
| recordtype | eprints |
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| spelling | upm-626152020-12-03T20:23:46Z http://psasir.upm.edu.my/id/eprint/62615/ Optimization of metallo-keratinase production by Pseudomonas sp. LM19 as a potential enzyme for feather waste conversion Mohamad, Nurliyana Phang, Lai Yee Abd Aziz, Suraini Locally isolated bacterium Pseudomonas sp. LM19, a metallo-keratinase producer was used to hydrolyze the highly rigid keratin recalcitrant in this study. The production of crude keratinase by Pseudomonas sp. LM19 is influenced by both physical and nutritional parameters. The highest keratinase activity of 127 U/ml (2.15-fold) was observed in feather meal medium supplemented with fructose and peptone at a C/N ratio of 40. The optimum pH and temperature for keratinase production were found to be pH 8 and 30 °C, using 1% (w/v) feather as substrate. The degradation rate of the feathers was increased 2.4-fold at optimized physical and nutritional conditions. Feather degradation by Pseudomonas sp. LM19 led to the production of free amino acids such as arginine, glycine, leucine, and serine. The information on the production of keratinase by Pseudomonas sp. LM19 obtained from this study warrants further research for possible commercial application. Informa Healthcare 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/62615/1/KERATIN.pdf Mohamad, Nurliyana and Phang, Lai Yee and Abd Aziz, Suraini (2017) Optimization of metallo-keratinase production by Pseudomonas sp. LM19 as a potential enzyme for feather waste conversion. Biocatalysis and Biotransformation, 35 (1). pp. 41-50. ISSN 1029-2446; ESSN: 1024-2422 https://www.tandfonline.com/doi/abs/10.1080/10242422.2017.1280031 10.1080/10242422.2017.1280031 |
| spellingShingle | Mohamad, Nurliyana Phang, Lai Yee Abd Aziz, Suraini Optimization of metallo-keratinase production by Pseudomonas sp. LM19 as a potential enzyme for feather waste conversion |
| title | Optimization of metallo-keratinase production by Pseudomonas sp. LM19 as a potential enzyme for feather waste conversion |
| title_full | Optimization of metallo-keratinase production by Pseudomonas sp. LM19 as a potential enzyme for feather waste conversion |
| title_fullStr | Optimization of metallo-keratinase production by Pseudomonas sp. LM19 as a potential enzyme for feather waste conversion |
| title_full_unstemmed | Optimization of metallo-keratinase production by Pseudomonas sp. LM19 as a potential enzyme for feather waste conversion |
| title_short | Optimization of metallo-keratinase production by Pseudomonas sp. LM19 as a potential enzyme for feather waste conversion |
| title_sort | optimization of metallo-keratinase production by pseudomonas sp. lm19 as a potential enzyme for feather waste conversion |
| url | http://psasir.upm.edu.my/id/eprint/62615/ http://psasir.upm.edu.my/id/eprint/62615/ http://psasir.upm.edu.my/id/eprint/62615/ http://psasir.upm.edu.my/id/eprint/62615/1/KERATIN.pdf |