Occurrence of Escherichia coli harbouring stx genes in popiah, a Malaysian street food
Irrespective of its health effects, street foods are very popular with the consumers. The main purpose of this research was to study the biosafety of Escherichia coli in popiah, a Malaysian street food sold at a roadside food stall and a restaurant in Sri Serdang, Selangor, Malaysia, using the combi...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Rynnye Lyan Resources
2017
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/62563/ http://psasir.upm.edu.my/id/eprint/62563/1/POPIAH.pdf |
| _version_ | 1848854639079849984 |
|---|---|
| author | Nillian, Elexson F. N., Nik Yuhanis Tan, Malcolm Turk Hsern C. Y., New Chang, W. S. Ubong, A. Kuan, Chee Hao Loo, Yuet Ying Thung, Tze Young Radu, Son |
| author_facet | Nillian, Elexson F. N., Nik Yuhanis Tan, Malcolm Turk Hsern C. Y., New Chang, W. S. Ubong, A. Kuan, Chee Hao Loo, Yuet Ying Thung, Tze Young Radu, Son |
| author_sort | Nillian, Elexson |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Irrespective of its health effects, street foods are very popular with the consumers. The main purpose of this research was to study the biosafety of Escherichia coli in popiah, a Malaysian street food sold at a roadside food stall and a restaurant in Sri Serdang, Selangor, Malaysia, using the combination of the most probable number (MPN)-Polymerase Chain Reaction (PCR) assay-plating on Eosin Methylene Blue (EMB) agar methods. Using these biomolecular methods, E. coli was detected in 12/15 (80%) and 11/15 (73%) of the collected samples from the roadside food stall and the restaurant respectively. The incidence of stx virulence-associated
genes was detected in 1/15 (7%) among the E. coli isolated from samples taken from the roadside food stall while the E. coli isolated from the restaurant was 3/15 (20%). The density of E. coli ranged from <3 to >1100 MPN/g and the density of E. coli positive with stx genes was <3 to 53 MPN /g in samples from both the roadside food stall and the restaurant. The presence of the stx-positive E. coli in popiah are significant to risk assessments of food and epidemiological studies. Therefore, from the information obtained in this study, it is obvious that the screening for STEC markers in food samples would be useful for food safety survey. |
| first_indexed | 2025-11-15T11:13:03Z |
| format | Article |
| id | upm-62563 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:13:03Z |
| publishDate | 2017 |
| publisher | Rynnye Lyan Resources |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-625632020-12-03T20:27:26Z http://psasir.upm.edu.my/id/eprint/62563/ Occurrence of Escherichia coli harbouring stx genes in popiah, a Malaysian street food Nillian, Elexson F. N., Nik Yuhanis Tan, Malcolm Turk Hsern C. Y., New Chang, W. S. Ubong, A. Kuan, Chee Hao Loo, Yuet Ying Thung, Tze Young Radu, Son Irrespective of its health effects, street foods are very popular with the consumers. The main purpose of this research was to study the biosafety of Escherichia coli in popiah, a Malaysian street food sold at a roadside food stall and a restaurant in Sri Serdang, Selangor, Malaysia, using the combination of the most probable number (MPN)-Polymerase Chain Reaction (PCR) assay-plating on Eosin Methylene Blue (EMB) agar methods. Using these biomolecular methods, E. coli was detected in 12/15 (80%) and 11/15 (73%) of the collected samples from the roadside food stall and the restaurant respectively. The incidence of stx virulence-associated genes was detected in 1/15 (7%) among the E. coli isolated from samples taken from the roadside food stall while the E. coli isolated from the restaurant was 3/15 (20%). The density of E. coli ranged from <3 to >1100 MPN/g and the density of E. coli positive with stx genes was <3 to 53 MPN /g in samples from both the roadside food stall and the restaurant. The presence of the stx-positive E. coli in popiah are significant to risk assessments of food and epidemiological studies. Therefore, from the information obtained in this study, it is obvious that the screening for STEC markers in food samples would be useful for food safety survey. Rynnye Lyan Resources 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/62563/1/POPIAH.pdf Nillian, Elexson and F. N., Nik Yuhanis and Tan, Malcolm Turk Hsern and C. Y., New and Chang, W. S. and Ubong, A. and Kuan, Chee Hao and Loo, Yuet Ying and Thung, Tze Young and Radu, Son (2017) Occurrence of Escherichia coli harbouring stx genes in popiah, a Malaysian street food. Food Research, 1 (1). pp. 29-32. ISSN 2550-2166 http://www.myfoodresearch.com/uploads/8/4/8/5/84855864/_5__fr-2017-008_elexson__eissn_.pdf 10.26656/ fr.2017.1.008 |
| spellingShingle | Nillian, Elexson F. N., Nik Yuhanis Tan, Malcolm Turk Hsern C. Y., New Chang, W. S. Ubong, A. Kuan, Chee Hao Loo, Yuet Ying Thung, Tze Young Radu, Son Occurrence of Escherichia coli harbouring stx genes in popiah, a Malaysian street food |
| title | Occurrence of Escherichia coli harbouring stx genes in popiah, a Malaysian street food |
| title_full | Occurrence of Escherichia coli harbouring stx genes in popiah, a Malaysian street food |
| title_fullStr | Occurrence of Escherichia coli harbouring stx genes in popiah, a Malaysian street food |
| title_full_unstemmed | Occurrence of Escherichia coli harbouring stx genes in popiah, a Malaysian street food |
| title_short | Occurrence of Escherichia coli harbouring stx genes in popiah, a Malaysian street food |
| title_sort | occurrence of escherichia coli harbouring stx genes in popiah, a malaysian street food |
| url | http://psasir.upm.edu.my/id/eprint/62563/ http://psasir.upm.edu.my/id/eprint/62563/ http://psasir.upm.edu.my/id/eprint/62563/ http://psasir.upm.edu.my/id/eprint/62563/1/POPIAH.pdf |