Magnetic micro-solid-phase extraction based on magnetite-MCM-41 with gas chromatography-mass spectrometry for the determination of antidepressant drugs in biological fluids
A new facile magnetic micro-solid-phase extraction coupled to gas chromatography and mass spectrometry detection was developed for the extraction and determination of selected antidepressant drugs in biological fluids using magnetite-MCM-41 as adsorbent. The synthesized sorbent was characterized by...
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| Format: | Article |
| Language: | English |
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Wiley-VCH Verlag
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/62328/ http://psasir.upm.edu.my/id/eprint/62328/1/Magnetic%20micro-solid-phase%20extraction%20based%20on%20magnetite-MCM-41%20with%20gas%20chromatography-mass%20spectrometry%20for%20the%20determination%20of%20antidepressant%20drugs%20in%20biological%20fluids.pdf |
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| author | Kamaruzaman, Sazlinda Sanagi, Mohd Marsin Yahaya, Noorfatimah Wan Ibrahim, Wan Aini Endud, Salasiah Wan Ibrahim, Wan Nazihah |
| author_facet | Kamaruzaman, Sazlinda Sanagi, Mohd Marsin Yahaya, Noorfatimah Wan Ibrahim, Wan Aini Endud, Salasiah Wan Ibrahim, Wan Nazihah |
| author_sort | Kamaruzaman, Sazlinda |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | A new facile magnetic micro-solid-phase extraction coupled to gas chromatography and mass spectrometry detection was developed for the extraction and determination of selected antidepressant drugs in biological fluids using magnetite-MCM-41 as adsorbent. The synthesized sorbent was characterized by several spectroscopic techniques. The maximum extraction efficiency for extraction of 500 μg/L antidepressant drugs from aqueous solution was obtained with 15 mg of magnetite-MCM-41 at pH 12. The analyte was desorbed using 100 μL of acetonitrile prior to gas chromatography determination. This method was rapid in which the adsorption procedure was completed in 60 s. Under the optimized conditions using 15 mL of antidepressant drugs sample, the calibration curve showed good linearity in the range of 0.05-500 μg/L (r2 = 0.996-0.999). Good limits of detection (0.008-0.010 μg/L) were obtained for the analytes with good relative standard deviations of <8.0% (n = 5) for the determination of 0.1, 5.0, and 500.0 μg/L of antidepressant drugs. This method was successfully applied to the determination of amitriptyline and chlorpromazine in plasma and urine samples. The recoveries of spiked plasma and urine samples were in the range of 86.1-115.4%. Results indicate that magnetite micro-solid-phase extraction with gas chromatography and mass spectrometry is a convenient, fast, and economical method for the extraction and determination of amitriptyline and chlorpromazine in biological samples. |
| first_indexed | 2025-11-15T11:12:34Z |
| format | Article |
| id | upm-62328 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:12:34Z |
| publishDate | 2017 |
| publisher | Wiley-VCH Verlag |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-623282022-05-20T01:31:56Z http://psasir.upm.edu.my/id/eprint/62328/ Magnetic micro-solid-phase extraction based on magnetite-MCM-41 with gas chromatography-mass spectrometry for the determination of antidepressant drugs in biological fluids Kamaruzaman, Sazlinda Sanagi, Mohd Marsin Yahaya, Noorfatimah Wan Ibrahim, Wan Aini Endud, Salasiah Wan Ibrahim, Wan Nazihah A new facile magnetic micro-solid-phase extraction coupled to gas chromatography and mass spectrometry detection was developed for the extraction and determination of selected antidepressant drugs in biological fluids using magnetite-MCM-41 as adsorbent. The synthesized sorbent was characterized by several spectroscopic techniques. The maximum extraction efficiency for extraction of 500 μg/L antidepressant drugs from aqueous solution was obtained with 15 mg of magnetite-MCM-41 at pH 12. The analyte was desorbed using 100 μL of acetonitrile prior to gas chromatography determination. This method was rapid in which the adsorption procedure was completed in 60 s. Under the optimized conditions using 15 mL of antidepressant drugs sample, the calibration curve showed good linearity in the range of 0.05-500 μg/L (r2 = 0.996-0.999). Good limits of detection (0.008-0.010 μg/L) were obtained for the analytes with good relative standard deviations of <8.0% (n = 5) for the determination of 0.1, 5.0, and 500.0 μg/L of antidepressant drugs. This method was successfully applied to the determination of amitriptyline and chlorpromazine in plasma and urine samples. The recoveries of spiked plasma and urine samples were in the range of 86.1-115.4%. Results indicate that magnetite micro-solid-phase extraction with gas chromatography and mass spectrometry is a convenient, fast, and economical method for the extraction and determination of amitriptyline and chlorpromazine in biological samples. Wiley-VCH Verlag 2017-11 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/62328/1/Magnetic%20micro-solid-phase%20extraction%20based%20on%20magnetite-MCM-41%20with%20gas%20chromatography-mass%20spectrometry%20for%20the%20determination%20of%20antidepressant%20drugs%20in%20biological%20fluids.pdf Kamaruzaman, Sazlinda and Sanagi, Mohd Marsin and Yahaya, Noorfatimah and Wan Ibrahim, Wan Aini and Endud, Salasiah and Wan Ibrahim, Wan Nazihah (2017) Magnetic micro-solid-phase extraction based on magnetite-MCM-41 with gas chromatography-mass spectrometry for the determination of antidepressant drugs in biological fluids. Journal of Separation Science, 40 (21). 4222 - 4232. ISSN 1615-9306; ESSN: 1615-9314 https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/jssc.201700549 10.1002/jssc.201700549 |
| spellingShingle | Kamaruzaman, Sazlinda Sanagi, Mohd Marsin Yahaya, Noorfatimah Wan Ibrahim, Wan Aini Endud, Salasiah Wan Ibrahim, Wan Nazihah Magnetic micro-solid-phase extraction based on magnetite-MCM-41 with gas chromatography-mass spectrometry for the determination of antidepressant drugs in biological fluids |
| title | Magnetic micro-solid-phase extraction based on magnetite-MCM-41 with gas chromatography-mass spectrometry for the determination of antidepressant drugs in biological fluids |
| title_full | Magnetic micro-solid-phase extraction based on magnetite-MCM-41 with gas chromatography-mass spectrometry for the determination of antidepressant drugs in biological fluids |
| title_fullStr | Magnetic micro-solid-phase extraction based on magnetite-MCM-41 with gas chromatography-mass spectrometry for the determination of antidepressant drugs in biological fluids |
| title_full_unstemmed | Magnetic micro-solid-phase extraction based on magnetite-MCM-41 with gas chromatography-mass spectrometry for the determination of antidepressant drugs in biological fluids |
| title_short | Magnetic micro-solid-phase extraction based on magnetite-MCM-41 with gas chromatography-mass spectrometry for the determination of antidepressant drugs in biological fluids |
| title_sort | magnetic micro-solid-phase extraction based on magnetite-mcm-41 with gas chromatography-mass spectrometry for the determination of antidepressant drugs in biological fluids |
| url | http://psasir.upm.edu.my/id/eprint/62328/ http://psasir.upm.edu.my/id/eprint/62328/ http://psasir.upm.edu.my/id/eprint/62328/ http://psasir.upm.edu.my/id/eprint/62328/1/Magnetic%20micro-solid-phase%20extraction%20based%20on%20magnetite-MCM-41%20with%20gas%20chromatography-mass%20spectrometry%20for%20the%20determination%20of%20antidepressant%20drugs%20in%20biological%20fluids.pdf |