Isolation of molybdenum-reducing bacterium; Serratia sp. strain MIE2 from agriculture soil and its potential use in soil bioremediation
Molybdenum is reported to be very toxic to ruminants and shows evidence of spermatogenesistoxicity in animals and insects. Hence, its removal is important. In this study, we report on thefirst isolation of molybdenum-reducing bacterium from agricultural soil. The bacterium reduceshexavalent molybden...
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Hibiscus Publisher
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/62240/ http://psasir.upm.edu.my/id/eprint/62240/1/Isolation%20of%20molybdenum-reducing%20bacterium.pdf |
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| author | Halmi, Mohd Izuan Effendi Wan Johari, Wan Lutfi Mohd Ali, Mohd Shukuri Shaharuddin, Noor Azmi |
| author_facet | Halmi, Mohd Izuan Effendi Wan Johari, Wan Lutfi Mohd Ali, Mohd Shukuri Shaharuddin, Noor Azmi |
| author_sort | Halmi, Mohd Izuan Effendi |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Molybdenum is reported to be very toxic to ruminants and shows evidence of spermatogenesistoxicity in animals and insects. Hence, its removal is important. In this study, we report on thefirst isolation of molybdenum-reducing bacterium from agricultural soil. The bacterium reduceshexavalent molybdenum (sodium molybdate) to molybdenum blue (Mo-blue); a colloidalproduct, which can be trapped and removed from solution. Phylogenetic analysis resulted in atentative identification of the bacterium as Serratia sp. strain MIE2. The optimum conditions forMo-blue production using the normal one-variable-at-a-time (OVAT) approach were 10 mM ofsodium molybdate, pH 6.0, a temperature of 35°C, ammonium sulphate at 10 g/L as the nitrogensource and sucrose concentrations of between 30-50 g/L as the carbon source and electron donorfor molybdate. Studies on the effects of pesticides and solvents on Mo-blue production showedthat Mo-blue production from whole cells was relatively more affected by these xenobioticscompared to the crude enzyme. Nevertheless, the strain was resistant to most of the xenobioticstested. Based on the strain MIE characteristics, the bacterium will be a suitable candidate for theremediation of aquatic bodies and agricultural soils contaminated with molybdenum. |
| first_indexed | 2025-11-15T11:12:18Z |
| format | Article |
| id | upm-62240 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:12:18Z |
| publishDate | 2017 |
| publisher | Hibiscus Publisher |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-622402019-05-21T06:08:45Z http://psasir.upm.edu.my/id/eprint/62240/ Isolation of molybdenum-reducing bacterium; Serratia sp. strain MIE2 from agriculture soil and its potential use in soil bioremediation Halmi, Mohd Izuan Effendi Wan Johari, Wan Lutfi Mohd Ali, Mohd Shukuri Shaharuddin, Noor Azmi Molybdenum is reported to be very toxic to ruminants and shows evidence of spermatogenesistoxicity in animals and insects. Hence, its removal is important. In this study, we report on thefirst isolation of molybdenum-reducing bacterium from agricultural soil. The bacterium reduceshexavalent molybdenum (sodium molybdate) to molybdenum blue (Mo-blue); a colloidalproduct, which can be trapped and removed from solution. Phylogenetic analysis resulted in atentative identification of the bacterium as Serratia sp. strain MIE2. The optimum conditions forMo-blue production using the normal one-variable-at-a-time (OVAT) approach were 10 mM ofsodium molybdate, pH 6.0, a temperature of 35°C, ammonium sulphate at 10 g/L as the nitrogensource and sucrose concentrations of between 30-50 g/L as the carbon source and electron donorfor molybdate. Studies on the effects of pesticides and solvents on Mo-blue production showedthat Mo-blue production from whole cells was relatively more affected by these xenobioticscompared to the crude enzyme. Nevertheless, the strain was resistant to most of the xenobioticstested. Based on the strain MIE characteristics, the bacterium will be a suitable candidate for theremediation of aquatic bodies and agricultural soils contaminated with molybdenum. Hibiscus Publisher 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/62240/1/Isolation%20of%20molybdenum-reducing%20bacterium.pdf Halmi, Mohd Izuan Effendi and Wan Johari, Wan Lutfi and Mohd Ali, Mohd Shukuri and Shaharuddin, Noor Azmi (2017) Isolation of molybdenum-reducing bacterium; Serratia sp. strain MIE2 from agriculture soil and its potential use in soil bioremediation. Journal of Biochemistry, Microbiology and Biotechnology, 5 (2). pp. 12-18. ISSN 2289-5779 https://journal.hibiscuspublisher.com/index.php/JOBIMB/article/view/342 |
| spellingShingle | Halmi, Mohd Izuan Effendi Wan Johari, Wan Lutfi Mohd Ali, Mohd Shukuri Shaharuddin, Noor Azmi Isolation of molybdenum-reducing bacterium; Serratia sp. strain MIE2 from agriculture soil and its potential use in soil bioremediation |
| title | Isolation of molybdenum-reducing bacterium; Serratia sp. strain MIE2 from agriculture soil and its potential use in soil bioremediation |
| title_full | Isolation of molybdenum-reducing bacterium; Serratia sp. strain MIE2 from agriculture soil and its potential use in soil bioremediation |
| title_fullStr | Isolation of molybdenum-reducing bacterium; Serratia sp. strain MIE2 from agriculture soil and its potential use in soil bioremediation |
| title_full_unstemmed | Isolation of molybdenum-reducing bacterium; Serratia sp. strain MIE2 from agriculture soil and its potential use in soil bioremediation |
| title_short | Isolation of molybdenum-reducing bacterium; Serratia sp. strain MIE2 from agriculture soil and its potential use in soil bioremediation |
| title_sort | isolation of molybdenum-reducing bacterium; serratia sp. strain mie2 from agriculture soil and its potential use in soil bioremediation |
| url | http://psasir.upm.edu.my/id/eprint/62240/ http://psasir.upm.edu.my/id/eprint/62240/ http://psasir.upm.edu.my/id/eprint/62240/1/Isolation%20of%20molybdenum-reducing%20bacterium.pdf |