Isolation and molecular identification of Colletotrichum gleosporiodes from infected peanut seeds

Peanut (Arachis hypogaea(L) cv. Margenta) seeds are currently, significantly considered as an important source of protein and vegetable oil. Anthracnose caused by Colletotrichum gloeosporioidesis a fungal disease from seed that reduces seedling emergence and seed germina...

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Main Authors: Rajeendran, Allegendran, Nulit, Rosimah, Yong, Christina Seok Yien, Ibrahim, Mohd. Hafiz, Kalhori, Nahid
Format: Article
Language:English
Published: SCIENCEDOMAIN international 2017
Online Access:http://psasir.upm.edu.my/id/eprint/62237/
http://psasir.upm.edu.my/id/eprint/62237/1/Isolation%20and%20molecular%20identification%20of%20Colletotrichum%20gleosporiodes.pdf
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author Rajeendran, Allegendran
Nulit, Rosimah
Yong, Christina Seok Yien
Ibrahim, Mohd. Hafiz
Kalhori, Nahid
author_facet Rajeendran, Allegendran
Nulit, Rosimah
Yong, Christina Seok Yien
Ibrahim, Mohd. Hafiz
Kalhori, Nahid
author_sort Rajeendran, Allegendran
building UPM Institutional Repository
collection Online Access
description Peanut (Arachis hypogaea(L) cv. Margenta) seeds are currently, significantly considered as an important source of protein and vegetable oil. Anthracnose caused by Colletotrichum gloeosporioidesis a fungal disease from seed that reduces seedling emergence and seed germination. The pathogenic fungi that isolated from peanut were identified using ITS 1and ITS 4 primer with polymerase chain reaction and NCBI database using BlastN algorithm. From NCBI using blastN analysed was 95% identify with other Colletotrichumspecies in NCBI database search. The pathogenicity of Colletotrichum gloeosporioideson peanut seeds and seedlings at 14 days after sowing result was inoculated with pathogenic fungi with 32.3% and control was 83%. Hence, this study was experimented with the object to reveal seed-borne infections on tropical peanut in Malaysia.
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institution Universiti Putra Malaysia
institution_category Local University
language English
last_indexed 2025-11-15T11:12:18Z
publishDate 2017
publisher SCIENCEDOMAIN international
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spelling upm-622372019-05-21T05:58:20Z http://psasir.upm.edu.my/id/eprint/62237/ Isolation and molecular identification of Colletotrichum gleosporiodes from infected peanut seeds Rajeendran, Allegendran Nulit, Rosimah Yong, Christina Seok Yien Ibrahim, Mohd. Hafiz Kalhori, Nahid Peanut (Arachis hypogaea(L) cv. Margenta) seeds are currently, significantly considered as an important source of protein and vegetable oil. Anthracnose caused by Colletotrichum gloeosporioidesis a fungal disease from seed that reduces seedling emergence and seed germination. The pathogenic fungi that isolated from peanut were identified using ITS 1and ITS 4 primer with polymerase chain reaction and NCBI database using BlastN algorithm. From NCBI using blastN analysed was 95% identify with other Colletotrichumspecies in NCBI database search. The pathogenicity of Colletotrichum gloeosporioideson peanut seeds and seedlings at 14 days after sowing result was inoculated with pathogenic fungi with 32.3% and control was 83%. Hence, this study was experimented with the object to reveal seed-borne infections on tropical peanut in Malaysia. SCIENCEDOMAIN international 2017-01 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/62237/1/Isolation%20and%20molecular%20identification%20of%20Colletotrichum%20gleosporiodes.pdf Rajeendran, Allegendran and Nulit, Rosimah and Yong, Christina Seok Yien and Ibrahim, Mohd. Hafiz and Kalhori, Nahid (2017) Isolation and molecular identification of Colletotrichum gleosporiodes from infected peanut seeds. International Journal of Plant & Soil Science, 19 (2). pp. 1-8. ISSN 2320-7035 http://www.journalijpss.com/index.php/IJPSS/issue/view/692 10.9734/IJPSS/2017/35838
spellingShingle Rajeendran, Allegendran
Nulit, Rosimah
Yong, Christina Seok Yien
Ibrahim, Mohd. Hafiz
Kalhori, Nahid
Isolation and molecular identification of Colletotrichum gleosporiodes from infected peanut seeds
title Isolation and molecular identification of Colletotrichum gleosporiodes from infected peanut seeds
title_full Isolation and molecular identification of Colletotrichum gleosporiodes from infected peanut seeds
title_fullStr Isolation and molecular identification of Colletotrichum gleosporiodes from infected peanut seeds
title_full_unstemmed Isolation and molecular identification of Colletotrichum gleosporiodes from infected peanut seeds
title_short Isolation and molecular identification of Colletotrichum gleosporiodes from infected peanut seeds
title_sort isolation and molecular identification of colletotrichum gleosporiodes from infected peanut seeds
url http://psasir.upm.edu.my/id/eprint/62237/
http://psasir.upm.edu.my/id/eprint/62237/
http://psasir.upm.edu.my/id/eprint/62237/
http://psasir.upm.edu.my/id/eprint/62237/1/Isolation%20and%20molecular%20identification%20of%20Colletotrichum%20gleosporiodes.pdf