Isolation and characterisation of a molybdenum-reducing and Metanil Yellow dye-decolourising Bacillus sp. strain neni-10 in soils from West Sumatera, Indonesia
A molybdenum reducing bacterium with the novel ability to decolorise the azo dye Metanil Yellow is reported. Optimal conditions for molybdenum reduction were pH 6.3 and at 34°C. Glucose was the best electron donor. Another requirement includes a narrow phosphate concentration between 2.5 and 7.5 mM....
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Sains Malaysia
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/62225/ http://psasir.upm.edu.my/id/eprint/62225/1/Isolation%20and%20characterisation%20of%20a%20molybdenum-reducing%20and%20Metanil%20Yellow%20dye-decolourising%20Bacillus%20sp.%20strain%20neni-10%20in%20soils%20from%20West%20Sumatera%2C%20Indonesia.pdf |
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| author | Mansur, Rusnam Gusmanizar, Neni Roslan, Muhamad Akhmal Hakim Ahmad, Siti Aqlima Shukor, Mohd Yunus |
| author_facet | Mansur, Rusnam Gusmanizar, Neni Roslan, Muhamad Akhmal Hakim Ahmad, Siti Aqlima Shukor, Mohd Yunus |
| author_sort | Mansur, Rusnam |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | A molybdenum reducing bacterium with the novel ability to decolorise the azo dye Metanil Yellow is reported. Optimal conditions for molybdenum reduction were pH 6.3 and at 34°C. Glucose was the best electron donor. Another requirement includes a narrow phosphate concentration between 2.5 and 7.5 mM. A time profile of Mo-blue production shows a lag period of approximately 12 hours, a maximum amount of Mo-blue produced at a molybdate concentration of 20 mM, and a peak production at 52 h of incubation. The heavy metals mercury, silver, copper and chromium inhibited reduction by 91.9, 82.7, 45.5 and 17.4%, respectively. A complete decolourisation of the dye Metanil Yellow at 100 and 150 mg/L occurred at day three and day six of incubations, respectively. Higher concentrations show partial degradation, with an approximately 20% decolourisation observed at 400 mg/L. The bacterium is partially identified based on biochemical analysis as Bacillus sp. strain Neni-10. The absorption spectrum of the Mo-blue suggested the compound is a reduced phosphomolybdate. The isolation of this bacterium, which shows heavy metal reduction and dye-decolorising ability, is sought after, particularly for bioremediation. |
| first_indexed | 2025-11-15T11:12:15Z |
| format | Article |
| id | upm-62225 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:12:15Z |
| publishDate | 2017 |
| publisher | Penerbit Universiti Sains Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-622252019-04-23T07:49:50Z http://psasir.upm.edu.my/id/eprint/62225/ Isolation and characterisation of a molybdenum-reducing and Metanil Yellow dye-decolourising Bacillus sp. strain neni-10 in soils from West Sumatera, Indonesia Mansur, Rusnam Gusmanizar, Neni Roslan, Muhamad Akhmal Hakim Ahmad, Siti Aqlima Shukor, Mohd Yunus A molybdenum reducing bacterium with the novel ability to decolorise the azo dye Metanil Yellow is reported. Optimal conditions for molybdenum reduction were pH 6.3 and at 34°C. Glucose was the best electron donor. Another requirement includes a narrow phosphate concentration between 2.5 and 7.5 mM. A time profile of Mo-blue production shows a lag period of approximately 12 hours, a maximum amount of Mo-blue produced at a molybdate concentration of 20 mM, and a peak production at 52 h of incubation. The heavy metals mercury, silver, copper and chromium inhibited reduction by 91.9, 82.7, 45.5 and 17.4%, respectively. A complete decolourisation of the dye Metanil Yellow at 100 and 150 mg/L occurred at day three and day six of incubations, respectively. Higher concentrations show partial degradation, with an approximately 20% decolourisation observed at 400 mg/L. The bacterium is partially identified based on biochemical analysis as Bacillus sp. strain Neni-10. The absorption spectrum of the Mo-blue suggested the compound is a reduced phosphomolybdate. The isolation of this bacterium, which shows heavy metal reduction and dye-decolorising ability, is sought after, particularly for bioremediation. Penerbit Universiti Sains Malaysia 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/62225/1/Isolation%20and%20characterisation%20of%20a%20molybdenum-reducing%20and%20Metanil%20Yellow%20dye-decolourising%20Bacillus%20sp.%20strain%20neni-10%20in%20soils%20from%20West%20Sumatera%2C%20Indonesia.pdf Mansur, Rusnam and Gusmanizar, Neni and Roslan, Muhamad Akhmal Hakim and Ahmad, Siti Aqlima and Shukor, Mohd Yunus (2017) Isolation and characterisation of a molybdenum-reducing and Metanil Yellow dye-decolourising Bacillus sp. strain neni-10 in soils from West Sumatera, Indonesia. Tropical Life Sciences Research, 28 (1). pp. 69-90. ISSN 1985-3718; ESSN: 2180-4249 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5300016/ |
| spellingShingle | Mansur, Rusnam Gusmanizar, Neni Roslan, Muhamad Akhmal Hakim Ahmad, Siti Aqlima Shukor, Mohd Yunus Isolation and characterisation of a molybdenum-reducing and Metanil Yellow dye-decolourising Bacillus sp. strain neni-10 in soils from West Sumatera, Indonesia |
| title | Isolation and characterisation of a molybdenum-reducing and Metanil Yellow dye-decolourising Bacillus sp. strain neni-10 in soils from West Sumatera, Indonesia |
| title_full | Isolation and characterisation of a molybdenum-reducing and Metanil Yellow dye-decolourising Bacillus sp. strain neni-10 in soils from West Sumatera, Indonesia |
| title_fullStr | Isolation and characterisation of a molybdenum-reducing and Metanil Yellow dye-decolourising Bacillus sp. strain neni-10 in soils from West Sumatera, Indonesia |
| title_full_unstemmed | Isolation and characterisation of a molybdenum-reducing and Metanil Yellow dye-decolourising Bacillus sp. strain neni-10 in soils from West Sumatera, Indonesia |
| title_short | Isolation and characterisation of a molybdenum-reducing and Metanil Yellow dye-decolourising Bacillus sp. strain neni-10 in soils from West Sumatera, Indonesia |
| title_sort | isolation and characterisation of a molybdenum-reducing and metanil yellow dye-decolourising bacillus sp. strain neni-10 in soils from west sumatera, indonesia |
| url | http://psasir.upm.edu.my/id/eprint/62225/ http://psasir.upm.edu.my/id/eprint/62225/ http://psasir.upm.edu.my/id/eprint/62225/1/Isolation%20and%20characterisation%20of%20a%20molybdenum-reducing%20and%20Metanil%20Yellow%20dye-decolourising%20Bacillus%20sp.%20strain%20neni-10%20in%20soils%20from%20West%20Sumatera%2C%20Indonesia.pdf |