Inhibitory activity of Ficus deltoidea var. trengganuensis Aqueous extract on lipopolysaccharide-induced TNF-α production from microglia
Objective: To explore the effect of Ficus deltoidea (FD) aqueous extracts on the release of tumor necrosis factor-α (TNF-α), the expression of CD40, and the morphology of microglial cells in lipopolysaccharide- (LPS-) activated BV2 cells. Methods. The cytotoxicity of FD extract was assessed by MTS s...
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| Format: | Article |
| Language: | English |
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Hindawi Publishing Corporation
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/62180/ http://psasir.upm.edu.my/id/eprint/62180/1/Inhibitory%20Activity%20of%20Ficus%20deltoidea.pdf |
| _version_ | 1848854578695503872 |
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| author | Wang, Huiling Vidyadaran, Sharmili Mohd Moklas, Mohamad Aris Baharuldin, Mohamad Taufik Hidayat |
| author_facet | Wang, Huiling Vidyadaran, Sharmili Mohd Moklas, Mohamad Aris Baharuldin, Mohamad Taufik Hidayat |
| author_sort | Wang, Huiling |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Objective: To explore the effect of Ficus deltoidea (FD) aqueous extracts on the release of tumor necrosis factor-α (TNF-α), the expression of CD40, and the morphology of microglial cells in lipopolysaccharide- (LPS-) activated BV2 cells. Methods. The cytotoxicity of FD extract was assessed by MTS solution. BV2 cells were divided into 5 experimental groups, intervened, respectively, by FD (4 mg/mL) and LPS + FD (0, 1, 2, and 4 mg/mL). Besides, a blank control group was set up without any intervention. TNF-α release was assessed by enzyme linked immunosorbent assay (ELISA). The expression of CD40 was examined by flow cytometry. Immunocytochemical staining was used to show the morphology of BV2 cells. Results: FD extract of different concentrations (1, 2, and 4 mg/mL) had no significant toxic effects on the BV2 cells. FD suppressed the activation of microglia in morphology and reduced TNF-α production and expression of CD40 induced by LPS. Conclusion. FD extract has a therapeutic potential against neuroinflammatory diseases. |
| first_indexed | 2025-11-15T11:12:06Z |
| format | Article |
| id | upm-62180 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:12:06Z |
| publishDate | 2017 |
| publisher | Hindawi Publishing Corporation |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-621802019-04-18T08:45:30Z http://psasir.upm.edu.my/id/eprint/62180/ Inhibitory activity of Ficus deltoidea var. trengganuensis Aqueous extract on lipopolysaccharide-induced TNF-α production from microglia Wang, Huiling Vidyadaran, Sharmili Mohd Moklas, Mohamad Aris Baharuldin, Mohamad Taufik Hidayat Objective: To explore the effect of Ficus deltoidea (FD) aqueous extracts on the release of tumor necrosis factor-α (TNF-α), the expression of CD40, and the morphology of microglial cells in lipopolysaccharide- (LPS-) activated BV2 cells. Methods. The cytotoxicity of FD extract was assessed by MTS solution. BV2 cells were divided into 5 experimental groups, intervened, respectively, by FD (4 mg/mL) and LPS + FD (0, 1, 2, and 4 mg/mL). Besides, a blank control group was set up without any intervention. TNF-α release was assessed by enzyme linked immunosorbent assay (ELISA). The expression of CD40 was examined by flow cytometry. Immunocytochemical staining was used to show the morphology of BV2 cells. Results: FD extract of different concentrations (1, 2, and 4 mg/mL) had no significant toxic effects on the BV2 cells. FD suppressed the activation of microglia in morphology and reduced TNF-α production and expression of CD40 induced by LPS. Conclusion. FD extract has a therapeutic potential against neuroinflammatory diseases. Hindawi Publishing Corporation 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/62180/1/Inhibitory%20Activity%20of%20Ficus%20deltoidea.pdf Wang, Huiling and Vidyadaran, Sharmili and Mohd Moklas, Mohamad Aris and Baharuldin, Mohamad Taufik Hidayat (2017) Inhibitory activity of Ficus deltoidea var. trengganuensis Aqueous extract on lipopolysaccharide-induced TNF-α production from microglia. Evidence-Based Complementary and Alternative Medicine, 2017. art. no. 2623163. pp. 1-7. ISSN 1741-427X; ESSN: 1741-4288 https://www.hindawi.com/journals/ecam/2017/2623163/ 10.1155/2017/2623163 |
| spellingShingle | Wang, Huiling Vidyadaran, Sharmili Mohd Moklas, Mohamad Aris Baharuldin, Mohamad Taufik Hidayat Inhibitory activity of Ficus deltoidea var. trengganuensis Aqueous extract on lipopolysaccharide-induced TNF-α production from microglia |
| title | Inhibitory activity of Ficus deltoidea var. trengganuensis Aqueous extract on lipopolysaccharide-induced TNF-α production from microglia |
| title_full | Inhibitory activity of Ficus deltoidea var. trengganuensis Aqueous extract on lipopolysaccharide-induced TNF-α production from microglia |
| title_fullStr | Inhibitory activity of Ficus deltoidea var. trengganuensis Aqueous extract on lipopolysaccharide-induced TNF-α production from microglia |
| title_full_unstemmed | Inhibitory activity of Ficus deltoidea var. trengganuensis Aqueous extract on lipopolysaccharide-induced TNF-α production from microglia |
| title_short | Inhibitory activity of Ficus deltoidea var. trengganuensis Aqueous extract on lipopolysaccharide-induced TNF-α production from microglia |
| title_sort | inhibitory activity of ficus deltoidea var. trengganuensis aqueous extract on lipopolysaccharide-induced tnf-α production from microglia |
| url | http://psasir.upm.edu.my/id/eprint/62180/ http://psasir.upm.edu.my/id/eprint/62180/ http://psasir.upm.edu.my/id/eprint/62180/ http://psasir.upm.edu.my/id/eprint/62180/1/Inhibitory%20Activity%20of%20Ficus%20deltoidea.pdf |