In vitro transfer of methicillin resistance determinants mecA from methicillin resistant Staphylococcus aureus (MRSA) to methicillin susceptible Staphylococcus aureus (MSSA)
Background: Staphylococcus aureus more than any other human pathogen is a better model for the study of the adaptive evolution of bacterial resistance to antibiotics, as it has demonstrated a remarkable ability in its response to new antibiotics. This study was designed to investigate the in vitro...
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| Format: | Article |
| Language: | English |
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BioMed Central
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/62139/ http://psasir.upm.edu.my/id/eprint/62139/1/In%20vitro%20transfer%20of%20methicillin.pdf |
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| author | Bitrus, Asinamai Athliamai Zakaria, Zunita Bejo, Siti Khairani Othman, Sarah Ahmad Nadzir, Nur Adilah |
| author_facet | Bitrus, Asinamai Athliamai Zakaria, Zunita Bejo, Siti Khairani Othman, Sarah Ahmad Nadzir, Nur Adilah |
| author_sort | Bitrus, Asinamai Athliamai |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Background: Staphylococcus aureus more than any other human pathogen is a better model for the study of the
adaptive evolution of bacterial resistance to antibiotics, as it has demonstrated a remarkable ability in its response to new antibiotics. This study was designed to investigate the in vitro transfer of mecA gene from methicillin resistant S. aureus to methicillin susceptible S. aureus. Result: The recipient transconjugants were resistant to erythromycin, cefpodoxime and were mecA positive. PCR amplification of mecA after mix culture plating on Luria Bertani agar containing 100 μg/mL showed that 75% of the donor and 58.3% of the recipient transconjugants were mecA positive. Additionally, 61.5% of both the donor cells and recipient transconjugants were mecA positive, while 46.2% and 41.75% of both donor and recipient transconjugants were mecA positive on LB agar containing 50 μg/mL and 30 μg/mL respectively.
Conclusion: In this study, the direction of transfer of phenotypic resistance as well as mecA was observed to have
occurred from the donor to the recipient strains. This study affirmed the importance of horizontal transfer events in the dissemination of antibiotics resistance among different strains of MRSA. |
| first_indexed | 2025-11-15T11:11:56Z |
| format | Article |
| id | upm-62139 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:11:56Z |
| publishDate | 2017 |
| publisher | BioMed Central |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-621392019-04-16T03:11:13Z http://psasir.upm.edu.my/id/eprint/62139/ In vitro transfer of methicillin resistance determinants mecA from methicillin resistant Staphylococcus aureus (MRSA) to methicillin susceptible Staphylococcus aureus (MSSA) Bitrus, Asinamai Athliamai Zakaria, Zunita Bejo, Siti Khairani Othman, Sarah Ahmad Nadzir, Nur Adilah Background: Staphylococcus aureus more than any other human pathogen is a better model for the study of the adaptive evolution of bacterial resistance to antibiotics, as it has demonstrated a remarkable ability in its response to new antibiotics. This study was designed to investigate the in vitro transfer of mecA gene from methicillin resistant S. aureus to methicillin susceptible S. aureus. Result: The recipient transconjugants were resistant to erythromycin, cefpodoxime and were mecA positive. PCR amplification of mecA after mix culture plating on Luria Bertani agar containing 100 μg/mL showed that 75% of the donor and 58.3% of the recipient transconjugants were mecA positive. Additionally, 61.5% of both the donor cells and recipient transconjugants were mecA positive, while 46.2% and 41.75% of both donor and recipient transconjugants were mecA positive on LB agar containing 50 μg/mL and 30 μg/mL respectively. Conclusion: In this study, the direction of transfer of phenotypic resistance as well as mecA was observed to have occurred from the donor to the recipient strains. This study affirmed the importance of horizontal transfer events in the dissemination of antibiotics resistance among different strains of MRSA. BioMed Central 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/62139/1/In%20vitro%20transfer%20of%20methicillin.pdf Bitrus, Asinamai Athliamai and Zakaria, Zunita and Bejo, Siti Khairani and Othman, Sarah and Ahmad Nadzir, Nur Adilah (2017) In vitro transfer of methicillin resistance determinants mecA from methicillin resistant Staphylococcus aureus (MRSA) to methicillin susceptible Staphylococcus aureus (MSSA). BMC Microbiology, 17 (1). pp. 1-10. ISSN 1471-2180 https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-017-0994-6 10.1186/s12866-017-0994-6 |
| spellingShingle | Bitrus, Asinamai Athliamai Zakaria, Zunita Bejo, Siti Khairani Othman, Sarah Ahmad Nadzir, Nur Adilah In vitro transfer of methicillin resistance determinants mecA from methicillin resistant Staphylococcus aureus (MRSA) to methicillin susceptible Staphylococcus aureus (MSSA) |
| title | In vitro transfer of methicillin resistance determinants mecA from methicillin resistant Staphylococcus aureus (MRSA) to methicillin susceptible Staphylococcus aureus (MSSA) |
| title_full | In vitro transfer of methicillin resistance determinants mecA from methicillin resistant Staphylococcus aureus (MRSA) to methicillin susceptible Staphylococcus aureus (MSSA) |
| title_fullStr | In vitro transfer of methicillin resistance determinants mecA from methicillin resistant Staphylococcus aureus (MRSA) to methicillin susceptible Staphylococcus aureus (MSSA) |
| title_full_unstemmed | In vitro transfer of methicillin resistance determinants mecA from methicillin resistant Staphylococcus aureus (MRSA) to methicillin susceptible Staphylococcus aureus (MSSA) |
| title_short | In vitro transfer of methicillin resistance determinants mecA from methicillin resistant Staphylococcus aureus (MRSA) to methicillin susceptible Staphylococcus aureus (MSSA) |
| title_sort | in vitro transfer of methicillin resistance determinants meca from methicillin resistant staphylococcus aureus (mrsa) to methicillin susceptible staphylococcus aureus (mssa) |
| url | http://psasir.upm.edu.my/id/eprint/62139/ http://psasir.upm.edu.my/id/eprint/62139/ http://psasir.upm.edu.my/id/eprint/62139/ http://psasir.upm.edu.my/id/eprint/62139/1/In%20vitro%20transfer%20of%20methicillin.pdf |