In situ PCR for detection and differentiation of Newcastle disease virus strains in Leghorn chickens
In experiment 1, six specific pathogen free (SPF) chickens were intra-nasally infected with velogenic (v) NDV strain with titre of 10⁵ EID50/0.1 mL and 6 non-infected birds were used as controls. Chickens were sacrificed at different times and tissue samples were collected for In situ PCR and immuno...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Online Journal of Veterinary Research
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/62131/ http://psasir.upm.edu.my/id/eprint/62131/1/In%20situ%20PCR%20for%20detection%20and%20differentiation%20of%20Newcastle%20disease%20virus%20strains%20in%20Leghorn%20chickens.pdf |
| _version_ | 1848854566418776064 |
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| author | Hussein, Elawad A. Bejo, Mohd Hair Omar, Abdul Rahman Arshad, Siti Suri Ideris, Aini |
| author_facet | Hussein, Elawad A. Bejo, Mohd Hair Omar, Abdul Rahman Arshad, Siti Suri Ideris, Aini |
| author_sort | Hussein, Elawad A. |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | In experiment 1, six specific pathogen free (SPF) chickens were intra-nasally infected with velogenic (v) NDV strain with titre of 10⁵ EID50/0.1 mL and 6 non-infected birds were used as controls. Chickens were sacrificed at different times and tissue samples were collected for In situ PCR and immunoperoxidase staining (IPS). In situ PCR was more sensitive (P < 0.05) than IPS for detection of NDV. In a 2nd experiment, In situ PCR was done to differentiate NDV strains. Groups of 5 SPF chickens each, were infected with velogenic (10⁵EID50/0.1 mL) or lentogenic (l) NDV (103.0 EID50/0.1 mL) strains. Non-infected birds were used as controls. After sampling of tissues, an In situ PCR was developed using specific velogenic and lentogenic strain probes. In situ PCR velogenic probe was positive only to tissues infected by velogenic strain whereas lentogenic probe only with lentogenic infected-tissues. The findings suggested that the In situ PCR differentiated lentogenic from velogenic NDV virus strains. |
| first_indexed | 2025-11-15T11:11:54Z |
| format | Article |
| id | upm-62131 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:11:54Z |
| publishDate | 2017 |
| publisher | Online Journal of Veterinary Research |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-621312019-04-15T08:06:47Z http://psasir.upm.edu.my/id/eprint/62131/ In situ PCR for detection and differentiation of Newcastle disease virus strains in Leghorn chickens Hussein, Elawad A. Bejo, Mohd Hair Omar, Abdul Rahman Arshad, Siti Suri Ideris, Aini In experiment 1, six specific pathogen free (SPF) chickens were intra-nasally infected with velogenic (v) NDV strain with titre of 10⁵ EID50/0.1 mL and 6 non-infected birds were used as controls. Chickens were sacrificed at different times and tissue samples were collected for In situ PCR and immunoperoxidase staining (IPS). In situ PCR was more sensitive (P < 0.05) than IPS for detection of NDV. In a 2nd experiment, In situ PCR was done to differentiate NDV strains. Groups of 5 SPF chickens each, were infected with velogenic (10⁵EID50/0.1 mL) or lentogenic (l) NDV (103.0 EID50/0.1 mL) strains. Non-infected birds were used as controls. After sampling of tissues, an In situ PCR was developed using specific velogenic and lentogenic strain probes. In situ PCR velogenic probe was positive only to tissues infected by velogenic strain whereas lentogenic probe only with lentogenic infected-tissues. The findings suggested that the In situ PCR differentiated lentogenic from velogenic NDV virus strains. Online Journal of Veterinary Research 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/62131/1/In%20situ%20PCR%20for%20detection%20and%20differentiation%20of%20Newcastle%20disease%20virus%20strains%20in%20Leghorn%20chickens.pdf Hussein, Elawad A. and Bejo, Mohd Hair and Omar, Abdul Rahman and Arshad, Siti Suri and Ideris, Aini (2017) In situ PCR for detection and differentiation of Newcastle disease virus strains in Leghorn chickens. Online Journal of Veterinary Research, 21 (10). 657 - 668. ISSN 1328-925X http://onljvetres.com/newcastlepcrabs2017.htm |
| spellingShingle | Hussein, Elawad A. Bejo, Mohd Hair Omar, Abdul Rahman Arshad, Siti Suri Ideris, Aini In situ PCR for detection and differentiation of Newcastle disease virus strains in Leghorn chickens |
| title | In situ PCR for detection and differentiation of Newcastle disease virus strains in Leghorn chickens |
| title_full | In situ PCR for detection and differentiation of Newcastle disease virus strains in Leghorn chickens |
| title_fullStr | In situ PCR for detection and differentiation of Newcastle disease virus strains in Leghorn chickens |
| title_full_unstemmed | In situ PCR for detection and differentiation of Newcastle disease virus strains in Leghorn chickens |
| title_short | In situ PCR for detection and differentiation of Newcastle disease virus strains in Leghorn chickens |
| title_sort | in situ pcr for detection and differentiation of newcastle disease virus strains in leghorn chickens |
| url | http://psasir.upm.edu.my/id/eprint/62131/ http://psasir.upm.edu.my/id/eprint/62131/ http://psasir.upm.edu.my/id/eprint/62131/1/In%20situ%20PCR%20for%20detection%20and%20differentiation%20of%20Newcastle%20disease%20virus%20strains%20in%20Leghorn%20chickens.pdf |