In situ PCR for detection and differentiation of infectious bursal disease virus strains in chickens

QR Code

In situ PCR for detection and differentiation of infectious bursal disease virus strains in chickens

An In situ PCR method for detection and differentiation of infectious bursal disease virus (IBDV) strains is described. In one study, 15 specific pathogen free (SPF) 14 day old chickens were infected orally with very virulent (vv) IBDV strain with a titre of 107.5 EID50/0.1 mL. Six non-infected chic...

Full description

Bibliographic Details
Main Authors:	Hussein, Elawad A., Bejo, Mohd Hair, Liew, Pit S., Omar, Abdul Rahman, Arshad, Siti Suri, Ideris, Aini
Format:	Article
Language:	English
Published:	Online Journal of Veterinary Research 2017
Online Access:	http://psasir.upm.edu.my/id/eprint/62130/ http://psasir.upm.edu.my/id/eprint/62130/1/In%20situ%20PCR%20for%20detection%20and%20differentiation%20of%20infectious%20bursal%20disease%20virus%20strains%20in%20chickens.pdf

Similar Items

Development of in situ PCR technique for detection of very virulent infectious bursal disease virus strain
by: Elawad, H. A., et al.
Published: (2013)

Infectious bursal disease virus tissue tropism and pathogenesis of the infection in chickens by application of in situ PCR, immunoperoxase and HE staining
by: Elawad, Hussein Abdallah, et al.
Published: (2019)

Development of in situ PCR for virus detection, strain differentiation and pathogenesis of newcastle disease and infectious bursal disease in chickens
by: Mahmoud, Hussein Abdallah Elawad
Published: (2017)

Scoring system for lesions induced by different strains of infectious bursal disease virus in chicken
by: Hussein, Elawad A., et al.
Published: (2020)

In situ PCR for detection and differentiation of Newcastle disease virus strains in Leghorn chickens
by: Hussein, Elawad A., et al.
Published: (2017)

Molecular differentiation of infectious Bursal Disease Virus IBDV) strains.
by: Omar, Abdul Rahman, et al.
Published: (2012)

Development of in situ pcr technique for detection of velogenic newcastle disease virus strain
by: Elawad, H. A., et al.
Published: (2013)

Vaccines and vaccination against infectious bursal disease of chickens: prospects and challenges
by: Liew, Pit Sze, et al.
Published: (2016)

Immunostimulatory effects of arginine in broiler chickens challenged with vaccine strain of infectious bursal disease virus
by: Emadi, Mozhdeh, et al.
Published: (2010)

Detection and distinguishing infectious bursal disease virus (IBDV) strains by molecular biology method
by: Omar, Abdul Rahman, et al.
Published: (2010)

Determination of velogenic Newcastle disease virus strain tissue tropism and disease pathogenesis by application of immunoperoxidase staining andin situ PCR
by: Mahmoud, Hussein Abdallah Elawad, et al.
Published: (2015)

Complete coding sequence and phylogenetic analyses of very virulent infectious bursal disease virus isolate of Malaysia
by: Liew, Pit Sze, et al.
Published: (2013)

Expression of segment a gene of UPM04/190 infectious bursal disease virus in Arabidopsis thaliana
by: Liew, Pit Sze, et al.
Published: (2015)

Pathogenicity and immunogenicity of infectious bursal disease virus in poultry
by: Bejo, Mohd Hair, et al.
Published: (1999)

Bursal transcriptome of different inbred chicken lines following infection with very virulent infectious bursal disease virus
by: Mohd Isa, Noor Farhanah, et al.
Published: (2015)

Comparison of Sybr Green I, ELISA and conventional agarose gel-based PCR in the detection of infectious bursal disease virus
by: Hamzah, Hairul Aini, et al.
Published: (2008)

Characterization of three infectious bursal disease virus isolates obtained from layer chickens in Iran.
by: M.A., Bahmaninejad, et al.
Published: (2008)

UPM93 infectious bursal disease (IBD) vaccine for chickens
by: Bejo, Mohd Hair
Published: (2005)

Development of SYBR green I based one-step real-time RT-PCR assay for the detection and differentiation of very virulent and classical strains of infectious bursal disease virus
by: Lih, Ling Kong, et al.
Published: (2009)

Base Usage and Dinucleotide Frequency of Infectious Bursal Disease Virus
by: Tan, Do Yew, et al.
Published: (2004)

Immunogenicity study of infectious bursal disease virus vaccines in commercial broiler chickens
by: Dastjerdi, P. Zarghami, et al.
Published: (2024)

Velogenic newcastle disease virus tissue tropism and pathogenesis of infection in chickens by application of in situ PCR, immunoperoxase staining and HE staining
by: Hussein, Elawad A., et al.
Published: (2019)

Performance of real-time polymerase chain reaction in the detection of infectious bursal disease virus
by: Hamzah, Hairul Aini, et al.
Published: (2004)

Characterization of chicken splenic-derived dendritic cells following vaccine and very virulent strains of infectious bursal disease virus infection
by: Yasmin, A. R., et al.
Published: (2016)

Liposomes as vaccine carrier in hatchery vaccination against infectious bursal disease in broiler chickens
by: Wahab, Mukminah Sakinah, et al.
Published: (2015)

Involvement of splenic dendritic cells during infectious bursal disease virus infection in chickens
by: Abd Rahman, Yasmin, et al.
Published: (2013)

Efficacy and safety of an inactivated novel variant infectious bursal disease virus in broiler chickens
by: Hair-Bejo, Mohd, et al.
Published: (2024)

Efficacy and safety of an inactivated novel variant infectious bursal disease virus in broiler chickens
by: Hair-Bejo, Mohd, et al.
Published: (2024)

Differential activation of intraepithelial lymphocyte-natural killer cells in chickens infected with very virulent and vaccine strains of infectious bursal disease virus
by: Jahromi, Mohammad Zareian, et al.
Published: (2018)

Scoring system for lesions induced by different strains of Newcastle disease virus in chicken
by: Hussein, Elawad A., et al.
Published: (2018)

Analysis of viral RNA and apoptotic cells in bursae following infection with vaccine and very virulent strains of infectious bursal disease virus
by: Kong, Lih Ling, et al.
Published: (2004)

Hatchery vaccination using liposomes as vaccine delivery against Infectious Bursal Disease in broiler chickens
by: Wahab, Mukminah Sakinah, et al.
Published: (2017)

Transcriptomic analysis on susceptibility of different inbred chicken lines towards very virulent infectious bursal disease virus infection
by: Mohd Isa, Nor Farah, et al.
Published: (2013)

Transcriptomic analysis on susceptibility of different inbred chicken lines towards very virulent infectious bursal disease virus infection
by: Mohd Isa, Noor Farhanah, et al.
Published: (2012)

Adaptation and molecular characterization of two Malaysian very virulent infectious bursal disease virus isolates adapted in BGM-70 cell line
by: Lawal, Nafi’u, et al.
Published: (2017)

Tryptophan Stimulates Immune Response in Broiler Chickens Challenged with Infectious Bursal Disease Vaccine
by: Mozhdeh, Emadi, et al.
Published: (2010)

Detection of infectious bursal disease virus using SYBR Green 1 based realtime polymerase chain reaction
by: Hamzah, Hairul Aini, et al.
Published: (2004)

Genetic diversity of recent infectious bursal disease viruses isolated from vaccinated poultry flocks in Malaysia
by: Aliyu, Hayatuddeen Bako, et al.
Published: (2021)

Immuno-protective effect of commercial IBD vaccines against emerging novel variant infectious bursal disease virus in specific-pathogen-free chickens
by: Aliyu, Hayatuddeen Bako, et al.
Published: (2024)

Bursal immunopathology responses of specific-pathogen-free chickens and red jungle fowl infected with very virulent infectious bursal disease virus
by: Omar, Abdul Rahman, et al.
Published: (2018)