Improved cultivation of gdhA derivative Pasteurella multocida B: 2 for high density of viable cells through in situ ammonium removal using cation-exchange resin for use as animal vaccine
Pasteurella multocida serotype B:2 is the causative agent of hemorrhagic septicemia, a fatal disease of cattle and buffaloes. A live attenuated vaccine, gdhA derivative P. multocida B:2 mutant, was created to defeat the disease. During the cultivation of P. multocida B:2 mutant, substantial amount o...
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| Format: | Article |
| Language: | English |
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Elsevier
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/62115/ http://psasir.upm.edu.my/id/eprint/62115/1/Improved%20cultivation%20of%20gdhA%20derivative%20Pasteurella%20multocida%20.pdf |
| _version_ | 1848854562217132032 |
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| author | Oslan, Siti Nur Hazwani Tan, Joo Shun Saad, Mohd Zamri Halim, Murni Ariff, Arbakariya |
| author_facet | Oslan, Siti Nur Hazwani Tan, Joo Shun Saad, Mohd Zamri Halim, Murni Ariff, Arbakariya |
| author_sort | Oslan, Siti Nur Hazwani |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Pasteurella multocida serotype B:2 is the causative agent of hemorrhagic septicemia, a fatal disease of cattle and buffaloes. A live attenuated vaccine, gdhA derivative P. multocida B:2 mutant, was created to defeat the disease. During the cultivation of P. multocida B:2 mutant, substantial amount of ammonium was accumulated in the culture, which greatly inhibited the growth of this bacterium. The feasibility of using integrated cultivation with in situ removal of ammonium by cation-exchange resin for the improvement of growth and viability of P. multocida cells was investigated. The ability of various cation-exchange resins, which include Amberlite IRC86, Amberlite IR120 H, and Dowex DRG8 H, to selectively adsorbed ammonium was first investigated using sorption isotherm experiments. Amberlite IRC86 has the highest ability for ammonium adsorption. The incorporation of 10 g/L of Amberlite IRC86 resin into the shake flask culture (100 mL) of P. multocida B:2 mutant, improved the final viable cell concentration (7.2 × 1010 cfu/mL) by about 13-fold compared to that obtained in cultivation without resin (5.5 × 109 cfu/mL). In cultivation with Amberlite IRC86 resin, approximately 41% of the ammonium accumulated in the culture was removed. |
| first_indexed | 2025-11-15T11:11:50Z |
| format | Article |
| id | upm-62115 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:11:50Z |
| publishDate | 2017 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-621152019-04-15T02:37:45Z http://psasir.upm.edu.my/id/eprint/62115/ Improved cultivation of gdhA derivative Pasteurella multocida B: 2 for high density of viable cells through in situ ammonium removal using cation-exchange resin for use as animal vaccine Oslan, Siti Nur Hazwani Tan, Joo Shun Saad, Mohd Zamri Halim, Murni Ariff, Arbakariya Pasteurella multocida serotype B:2 is the causative agent of hemorrhagic septicemia, a fatal disease of cattle and buffaloes. A live attenuated vaccine, gdhA derivative P. multocida B:2 mutant, was created to defeat the disease. During the cultivation of P. multocida B:2 mutant, substantial amount of ammonium was accumulated in the culture, which greatly inhibited the growth of this bacterium. The feasibility of using integrated cultivation with in situ removal of ammonium by cation-exchange resin for the improvement of growth and viability of P. multocida cells was investigated. The ability of various cation-exchange resins, which include Amberlite IRC86, Amberlite IR120 H, and Dowex DRG8 H, to selectively adsorbed ammonium was first investigated using sorption isotherm experiments. Amberlite IRC86 has the highest ability for ammonium adsorption. The incorporation of 10 g/L of Amberlite IRC86 resin into the shake flask culture (100 mL) of P. multocida B:2 mutant, improved the final viable cell concentration (7.2 × 1010 cfu/mL) by about 13-fold compared to that obtained in cultivation without resin (5.5 × 109 cfu/mL). In cultivation with Amberlite IRC86 resin, approximately 41% of the ammonium accumulated in the culture was removed. Elsevier 2017-05 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/62115/1/Improved%20cultivation%20of%20gdhA%20derivative%20Pasteurella%20multocida%20.pdf Oslan, Siti Nur Hazwani and Tan, Joo Shun and Saad, Mohd Zamri and Halim, Murni and Ariff, Arbakariya (2017) Improved cultivation of gdhA derivative Pasteurella multocida B: 2 for high density of viable cells through in situ ammonium removal using cation-exchange resin for use as animal vaccine. Process Biochemistry, 56. pp. 1-7. ISSN 1359-5113; ESSN: 1873-3298 https://www.sciencedirect.com/science/article/pii/S1359511316305839 10.1016/j.procbio.2017.02.016 |
| spellingShingle | Oslan, Siti Nur Hazwani Tan, Joo Shun Saad, Mohd Zamri Halim, Murni Ariff, Arbakariya Improved cultivation of gdhA derivative Pasteurella multocida B: 2 for high density of viable cells through in situ ammonium removal using cation-exchange resin for use as animal vaccine |
| title | Improved cultivation of gdhA derivative Pasteurella multocida B: 2 for high density of viable cells through in situ ammonium removal using cation-exchange resin for use as animal vaccine |
| title_full | Improved cultivation of gdhA derivative Pasteurella multocida B: 2 for high density of viable cells through in situ ammonium removal using cation-exchange resin for use as animal vaccine |
| title_fullStr | Improved cultivation of gdhA derivative Pasteurella multocida B: 2 for high density of viable cells through in situ ammonium removal using cation-exchange resin for use as animal vaccine |
| title_full_unstemmed | Improved cultivation of gdhA derivative Pasteurella multocida B: 2 for high density of viable cells through in situ ammonium removal using cation-exchange resin for use as animal vaccine |
| title_short | Improved cultivation of gdhA derivative Pasteurella multocida B: 2 for high density of viable cells through in situ ammonium removal using cation-exchange resin for use as animal vaccine |
| title_sort | improved cultivation of gdha derivative pasteurella multocida b: 2 for high density of viable cells through in situ ammonium removal using cation-exchange resin for use as animal vaccine |
| url | http://psasir.upm.edu.my/id/eprint/62115/ http://psasir.upm.edu.my/id/eprint/62115/ http://psasir.upm.edu.my/id/eprint/62115/ http://psasir.upm.edu.my/id/eprint/62115/1/Improved%20cultivation%20of%20gdhA%20derivative%20Pasteurella%20multocida%20.pdf |