Impact of signal peptide and transmembrane segments on expression and biochemical properties of a lipase from Bacillus sphaericus 205y

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Impact of signal peptide and transmembrane segments on expression and biochemical properties of a lipase from Bacillus sphaericus 205y

A total of 97 amino acids, considered as the signal peptide and transmembrane segments were removed from 205y lipase gene using polymerase chain reaction technique that abolished the low activity of this enzyme. The mature enzyme was expressed in Escherichia coliusing pBAD expression vector, which g...

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Bibliographic Details
Main Authors:	Masomian, Malihe, Jasni, Azmiza Syawani, Raja Abdul Rahman, Raja Noor Zaliha, Salleh, Abu Bakar, Basri, Mahiran
Format:	Article
Language:	English
Published:	Elsevier 2017
Online Access:	http://psasir.upm.edu.my/id/eprint/62103/ http://psasir.upm.edu.my/id/eprint/62103/1/Impact%20of%20signal%20peptide%20and%20transmembrane%20segments%20on%20expression%20and%20biochemical%20properties%20of%20a%20lipase%20from%20Bacillus%20sphaericus%20205y.pdf

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