Humanizing glycosylation pathways in eukaryotic expression systems
Glycosylation represents the most widespread posttranslational modifications, found in a broad spectrum of natural and therapeutic recombinant proteins. It highly affects bioactivity, site-specificity, stability, solubility, immunogenicity, and serum half-life of glycoproteins. Numerous expression h...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Springer
2017
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/62054/ http://psasir.upm.edu.my/id/eprint/62054/1/Humanizing%20glycosylation%20pathways%20in%20eukaryotic%20expression%20systems.pdf |
| _version_ | 1848854548880293888 |
|---|---|
| author | Khan, Amjad Hayat Bayat, Hadi Rajabibazl, Masoumeh Sabri, Suriana Rahimpour, Azam |
| author_facet | Khan, Amjad Hayat Bayat, Hadi Rajabibazl, Masoumeh Sabri, Suriana Rahimpour, Azam |
| author_sort | Khan, Amjad Hayat |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Glycosylation represents the most widespread posttranslational modifications, found in a broad spectrum of natural and therapeutic recombinant proteins. It highly affects bioactivity, site-specificity, stability, solubility, immunogenicity, and serum half-life of glycoproteins. Numerous expression hosts including yeasts, insect cells, transgenic plants, and mammalian cells have been explored for synthesizing therapeutic glycoproteins. However, glycosylation profile of eukaryotic expression systems differs from human. Glycosylation strategies have been proposed for humanizing the glycosylation pathways in expression hosts which is the main theme of this review. Besides, we also highlighted the glycosylation potential of protozoan parasites by emphasizing on the mammalian-like glycosylation potential of Leishmania tarentolae known as Leishmania expression system. |
| first_indexed | 2025-11-15T11:11:37Z |
| format | Article |
| id | upm-62054 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:11:37Z |
| publishDate | 2017 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-620542019-03-21T01:32:56Z http://psasir.upm.edu.my/id/eprint/62054/ Humanizing glycosylation pathways in eukaryotic expression systems Khan, Amjad Hayat Bayat, Hadi Rajabibazl, Masoumeh Sabri, Suriana Rahimpour, Azam Glycosylation represents the most widespread posttranslational modifications, found in a broad spectrum of natural and therapeutic recombinant proteins. It highly affects bioactivity, site-specificity, stability, solubility, immunogenicity, and serum half-life of glycoproteins. Numerous expression hosts including yeasts, insect cells, transgenic plants, and mammalian cells have been explored for synthesizing therapeutic glycoproteins. However, glycosylation profile of eukaryotic expression systems differs from human. Glycosylation strategies have been proposed for humanizing the glycosylation pathways in expression hosts which is the main theme of this review. Besides, we also highlighted the glycosylation potential of protozoan parasites by emphasizing on the mammalian-like glycosylation potential of Leishmania tarentolae known as Leishmania expression system. Springer 2017-01 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/62054/1/Humanizing%20glycosylation%20pathways%20in%20eukaryotic%20expression%20systems.pdf Khan, Amjad Hayat and Bayat, Hadi and Rajabibazl, Masoumeh and Sabri, Suriana and Rahimpour, Azam (2017) Humanizing glycosylation pathways in eukaryotic expression systems. World Journal of Microbiology and Biotechnology, 33 (4). pp. 1-12. ISSN 0959-3993; ESSN: 1573-0972 https://link.springer.com/article/10.1007%2Fs11274-016-2172-7 10.1007/s11274-016-2172-7 |
| spellingShingle | Khan, Amjad Hayat Bayat, Hadi Rajabibazl, Masoumeh Sabri, Suriana Rahimpour, Azam Humanizing glycosylation pathways in eukaryotic expression systems |
| title | Humanizing glycosylation pathways in eukaryotic expression systems |
| title_full | Humanizing glycosylation pathways in eukaryotic expression systems |
| title_fullStr | Humanizing glycosylation pathways in eukaryotic expression systems |
| title_full_unstemmed | Humanizing glycosylation pathways in eukaryotic expression systems |
| title_short | Humanizing glycosylation pathways in eukaryotic expression systems |
| title_sort | humanizing glycosylation pathways in eukaryotic expression systems |
| url | http://psasir.upm.edu.my/id/eprint/62054/ http://psasir.upm.edu.my/id/eprint/62054/ http://psasir.upm.edu.my/id/eprint/62054/ http://psasir.upm.edu.my/id/eprint/62054/1/Humanizing%20glycosylation%20pathways%20in%20eukaryotic%20expression%20systems.pdf |