Human mesenchymal stem cells promote CD34þhematopoietic stemcell proliferation with preserved red blood cell differentiation capacity
Studies showed that co-transplantation of mesenchymal stem cells (MSCs) and cord blood-derived CD34+ hematopoietic stem cells (HSCs) offered greater therapeutic effects but little is known regarding the effects of human Wharton's jelly derived MSCs on HSC expansion and red blood cell (RBC) gene...
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| Format: | Article |
| Language: | English |
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International Federation for Cell Biology
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/62053/ http://psasir.upm.edu.my/id/eprint/62053/1/Human%20mesenchymal%20stem%20cells%20promote.pdf |
| _version_ | 1848854548587741184 |
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| author | Lau, Show Xuan Leong, Yin Yee Ng, Wai Hoe Ng, Albert Wee Po Ismail, Ida Shazrina Mohd Yusoff, Narazah Ramasamy, Rajesh Tan, Jun Jie |
| author_facet | Lau, Show Xuan Leong, Yin Yee Ng, Wai Hoe Ng, Albert Wee Po Ismail, Ida Shazrina Mohd Yusoff, Narazah Ramasamy, Rajesh Tan, Jun Jie |
| author_sort | Lau, Show Xuan |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Studies showed that co-transplantation of mesenchymal stem cells (MSCs) and cord blood-derived CD34+ hematopoietic stem cells (HSCs) offered greater therapeutic effects but little is known regarding the effects of human Wharton's jelly derived MSCs on HSC expansion and red blood cell (RBC) generation in vitro. This study aimed to investigate the effects of MSCs on HSC expansion and differentiation. HSCs were co-cultured with MSCs or with 10% MSCs-derived conditioned medium, with HSCs cultured under standard medium served as a control. Cell expansion rates, number of mononuclear cell post-expansion and number of enucleated cells post-differentiation were evaluated. HSCs showed superior proliferation in the presence of MSC with mean expansion rate of 3.5 × 108 ± 1.8 × 107 after day 7 compared to the conditioned medium and the control group (8.9 × 107 ± 1.1 × 108 and 7.0 × 107 ± 3.3 × 106 respectively, P < 0.001). Although no significant differences in RBC differentiation were observed between groups at passage IV, the number of enucleated cell was greater compared to earlier passages, indicating successful RBC differentiation. Cord blood-derived CD34+ HSCs can be greatly expanded by co-culturing with MSCs without affecting the RBC differentiation capability, suggesting the importance of direct MSC-HSCs contact in HSC expansion and RBC differentiation. |
| first_indexed | 2025-11-15T11:11:37Z |
| format | Article |
| id | upm-62053 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:11:37Z |
| publishDate | 2017 |
| publisher | International Federation for Cell Biology |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-620532019-03-21T01:16:46Z http://psasir.upm.edu.my/id/eprint/62053/ Human mesenchymal stem cells promote CD34þhematopoietic stemcell proliferation with preserved red blood cell differentiation capacity Lau, Show Xuan Leong, Yin Yee Ng, Wai Hoe Ng, Albert Wee Po Ismail, Ida Shazrina Mohd Yusoff, Narazah Ramasamy, Rajesh Tan, Jun Jie Studies showed that co-transplantation of mesenchymal stem cells (MSCs) and cord blood-derived CD34+ hematopoietic stem cells (HSCs) offered greater therapeutic effects but little is known regarding the effects of human Wharton's jelly derived MSCs on HSC expansion and red blood cell (RBC) generation in vitro. This study aimed to investigate the effects of MSCs on HSC expansion and differentiation. HSCs were co-cultured with MSCs or with 10% MSCs-derived conditioned medium, with HSCs cultured under standard medium served as a control. Cell expansion rates, number of mononuclear cell post-expansion and number of enucleated cells post-differentiation were evaluated. HSCs showed superior proliferation in the presence of MSC with mean expansion rate of 3.5 × 108 ± 1.8 × 107 after day 7 compared to the conditioned medium and the control group (8.9 × 107 ± 1.1 × 108 and 7.0 × 107 ± 3.3 × 106 respectively, P < 0.001). Although no significant differences in RBC differentiation were observed between groups at passage IV, the number of enucleated cell was greater compared to earlier passages, indicating successful RBC differentiation. Cord blood-derived CD34+ HSCs can be greatly expanded by co-culturing with MSCs without affecting the RBC differentiation capability, suggesting the importance of direct MSC-HSCs contact in HSC expansion and RBC differentiation. International Federation for Cell Biology 2017-06 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/62053/1/Human%20mesenchymal%20stem%20cells%20promote.pdf Lau, Show Xuan and Leong, Yin Yee and Ng, Wai Hoe and Ng, Albert Wee Po and Ismail, Ida Shazrina and Mohd Yusoff, Narazah and Ramasamy, Rajesh and Tan, Jun Jie (2017) Human mesenchymal stem cells promote CD34þhematopoietic stemcell proliferation with preserved red blood cell differentiation capacity. Cell Biology International, 41 (6). 697 - 704. ISSN 1065-6995; ESSN: 1095-8355 https://onlinelibrary.wiley.com/doi/full/10.1002/cbin.10774 10.1002/cbin.10774 |
| spellingShingle | Lau, Show Xuan Leong, Yin Yee Ng, Wai Hoe Ng, Albert Wee Po Ismail, Ida Shazrina Mohd Yusoff, Narazah Ramasamy, Rajesh Tan, Jun Jie Human mesenchymal stem cells promote CD34þhematopoietic stemcell proliferation with preserved red blood cell differentiation capacity |
| title | Human mesenchymal stem cells promote CD34þhematopoietic stemcell proliferation with preserved red blood cell differentiation capacity |
| title_full | Human mesenchymal stem cells promote CD34þhematopoietic stemcell proliferation with preserved red blood cell differentiation capacity |
| title_fullStr | Human mesenchymal stem cells promote CD34þhematopoietic stemcell proliferation with preserved red blood cell differentiation capacity |
| title_full_unstemmed | Human mesenchymal stem cells promote CD34þhematopoietic stemcell proliferation with preserved red blood cell differentiation capacity |
| title_short | Human mesenchymal stem cells promote CD34þhematopoietic stemcell proliferation with preserved red blood cell differentiation capacity |
| title_sort | human mesenchymal stem cells promote cd34þhematopoietic stemcell proliferation with preserved red blood cell differentiation capacity |
| url | http://psasir.upm.edu.my/id/eprint/62053/ http://psasir.upm.edu.my/id/eprint/62053/ http://psasir.upm.edu.my/id/eprint/62053/ http://psasir.upm.edu.my/id/eprint/62053/1/Human%20mesenchymal%20stem%20cells%20promote.pdf |