Hemogram responses in goats toward challenged with Corynebacterium pseudotuberculosis and its immunogen mycolic acids

Aim: This study was conducted to analyze the changes in blood profile of goats inoculated with Corynebacterium pseudotuberculosis and its immunogen mycolic acid (MA) extract. Materials and Methods: A total of 12 clinically healthy crossbred Boer female goats were divided into three groups; A, B and...

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Main Authors: Ahmed Odhah, Mohammed Naji, Abdullah, Faez Firdaus Jesse, Haron, Abd Wahid, Mohd. Lila, Mohd. Azmi, Saad, Mohd. Zamri, Khuder, Zaid, Hambali, Idris Umar, Umar, Muhammed, Mohammed Saleh, Wessam Monther
Format: Article
Language:English
Published: Veterinary World 2017
Online Access:http://psasir.upm.edu.my/id/eprint/62022/
http://psasir.upm.edu.my/id/eprint/62022/1/Hemogram%20responses%20in%20goats%20toward%20challenged%20with%20Corynebacterium%20pseudotuberculosis%20and%20its%20immunogen%20mycolic%20acids.pdf
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author Ahmed Odhah, Mohammed Naji
Abdullah, Faez Firdaus Jesse
Haron, Abd Wahid
Mohd. Lila, Mohd. Azmi
Saad, Mohd. Zamri
Khuder, Zaid
Hambali, Idris Umar
Umar, Muhammed
Mohammed Saleh, Wessam Monther
author_facet Ahmed Odhah, Mohammed Naji
Abdullah, Faez Firdaus Jesse
Haron, Abd Wahid
Mohd. Lila, Mohd. Azmi
Saad, Mohd. Zamri
Khuder, Zaid
Hambali, Idris Umar
Umar, Muhammed
Mohammed Saleh, Wessam Monther
author_sort Ahmed Odhah, Mohammed Naji
building UPM Institutional Repository
collection Online Access
description Aim: This study was conducted to analyze the changes in blood profile of goats inoculated with Corynebacterium pseudotuberculosis and its immunogen mycolic acid (MA) extract. Materials and Methods: A total of 12 clinically healthy crossbred Boer female goats were divided into three groups; A, B and C (4 goats each per group). Group A was inoculated with 2 ml sterile phosphate buffered saline via intradermal route as the negative control group whilst Group B was inoculated with 2 ml of MA extract (1 g/ml) intradermally and Group C was then inoculated with 2 ml (1×109) colony forming unit of active C. pseudotuberculosis intradermally. Blood sample was collected aseptically from the jugular vein periodically for complete blood count (CBC) analysis throughout the experimental period (3 months). Result: A significant decrease (p<0.05) was observed in red blood cells, hemoglobin (Hb), packed cell volume, mean corpuscular volume and mean corpuscular Hb concentration in Groups B and C as compared to the control while WBCs, neutrophil, lymphocyte and basophil showed a significant increase (p<0.05) as compared to the control. Conclusion: The inoculation of C. pseudotuberculosis and MA resulted in a significant change in the CBC, thereby, indicating that MA has a role in caseous lymphadenitis pathogenesis.
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spelling upm-620222019-03-18T04:13:19Z http://psasir.upm.edu.my/id/eprint/62022/ Hemogram responses in goats toward challenged with Corynebacterium pseudotuberculosis and its immunogen mycolic acids Ahmed Odhah, Mohammed Naji Abdullah, Faez Firdaus Jesse Haron, Abd Wahid Mohd. Lila, Mohd. Azmi Saad, Mohd. Zamri Khuder, Zaid Hambali, Idris Umar Umar, Muhammed Mohammed Saleh, Wessam Monther Aim: This study was conducted to analyze the changes in blood profile of goats inoculated with Corynebacterium pseudotuberculosis and its immunogen mycolic acid (MA) extract. Materials and Methods: A total of 12 clinically healthy crossbred Boer female goats were divided into three groups; A, B and C (4 goats each per group). Group A was inoculated with 2 ml sterile phosphate buffered saline via intradermal route as the negative control group whilst Group B was inoculated with 2 ml of MA extract (1 g/ml) intradermally and Group C was then inoculated with 2 ml (1×109) colony forming unit of active C. pseudotuberculosis intradermally. Blood sample was collected aseptically from the jugular vein periodically for complete blood count (CBC) analysis throughout the experimental period (3 months). Result: A significant decrease (p<0.05) was observed in red blood cells, hemoglobin (Hb), packed cell volume, mean corpuscular volume and mean corpuscular Hb concentration in Groups B and C as compared to the control while WBCs, neutrophil, lymphocyte and basophil showed a significant increase (p<0.05) as compared to the control. Conclusion: The inoculation of C. pseudotuberculosis and MA resulted in a significant change in the CBC, thereby, indicating that MA has a role in caseous lymphadenitis pathogenesis. Veterinary World 2017-06 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/62022/1/Hemogram%20responses%20in%20goats%20toward%20challenged%20with%20Corynebacterium%20pseudotuberculosis%20and%20its%20immunogen%20mycolic%20acids.pdf Ahmed Odhah, Mohammed Naji and Abdullah, Faez Firdaus Jesse and Haron, Abd Wahid and Mohd. Lila, Mohd. Azmi and Saad, Mohd. Zamri and Khuder, Zaid and Hambali, Idris Umar and Umar, Muhammed and Mohammed Saleh, Wessam Monther (2017) Hemogram responses in goats toward challenged with Corynebacterium pseudotuberculosis and its immunogen mycolic acids. Veterinary World, 10 (6). 655 - 661. ISSN 0972-8988; ESSN: 2231-0916 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5499083/ 10.14202/vetworld.2017.655-661
spellingShingle Ahmed Odhah, Mohammed Naji
Abdullah, Faez Firdaus Jesse
Haron, Abd Wahid
Mohd. Lila, Mohd. Azmi
Saad, Mohd. Zamri
Khuder, Zaid
Hambali, Idris Umar
Umar, Muhammed
Mohammed Saleh, Wessam Monther
Hemogram responses in goats toward challenged with Corynebacterium pseudotuberculosis and its immunogen mycolic acids
title Hemogram responses in goats toward challenged with Corynebacterium pseudotuberculosis and its immunogen mycolic acids
title_full Hemogram responses in goats toward challenged with Corynebacterium pseudotuberculosis and its immunogen mycolic acids
title_fullStr Hemogram responses in goats toward challenged with Corynebacterium pseudotuberculosis and its immunogen mycolic acids
title_full_unstemmed Hemogram responses in goats toward challenged with Corynebacterium pseudotuberculosis and its immunogen mycolic acids
title_short Hemogram responses in goats toward challenged with Corynebacterium pseudotuberculosis and its immunogen mycolic acids
title_sort hemogram responses in goats toward challenged with corynebacterium pseudotuberculosis and its immunogen mycolic acids
url http://psasir.upm.edu.my/id/eprint/62022/
http://psasir.upm.edu.my/id/eprint/62022/
http://psasir.upm.edu.my/id/eprint/62022/
http://psasir.upm.edu.my/id/eprint/62022/1/Hemogram%20responses%20in%20goats%20toward%20challenged%20with%20Corynebacterium%20pseudotuberculosis%20and%20its%20immunogen%20mycolic%20acids.pdf