Group B adenovirus enadenotucirev infects polarised colorectal cancer cells efficiently from the basolateral surface expected to be encountered during intravenous delivery to treat disseminated cancer
Enadenotucirev (EnAd) is a group B oncolytic adenovirus developed for systemic delivery and currently undergoing clinical evaluation for advanced cancer therapy. For differentiated carcinomas, systemic delivery would likely expose virus particles to the basolateral surface of cancer cells rather tha...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2017
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/61987/ http://psasir.upm.edu.my/id/eprint/61987/1/Group%20B%20adenovirus%20enadenotucirev%20infects%20polarised%20colorectal%20cancer%20cells%20.pdf |
| _version_ | 1848854532626317312 |
|---|---|
| author | Chia, Suet Lin Lei, Janet Ferguson, David J. P. Dyer, Arthur Fishera, Kerry D. Seymour, Leonard W. |
| author_facet | Chia, Suet Lin Lei, Janet Ferguson, David J. P. Dyer, Arthur Fishera, Kerry D. Seymour, Leonard W. |
| author_sort | Chia, Suet Lin |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Enadenotucirev (EnAd) is a group B oncolytic adenovirus developed for systemic delivery and currently undergoing clinical evaluation for advanced cancer therapy. For differentiated carcinomas, systemic delivery would likely expose virus particles to the basolateral surface of cancer cells rather than the apical surface encountered during natural infection. Here, we compare the ability of EnAd and adenovirus type-5 (Ad5) to infect polarised colorectal carcinoma cells from the apical or basolateral surfaces. Whereas Ad5 infection was more efficient via the apical than basolateral surface, EnAd readily infected cells from either surface. Progeny particles from EnAd were released preferentially via the apical surface for all cell lines and routes of infection. These data further support the utility of group B adenoviruses for systemic delivery and suggest that progeny virus are more likely to be released into the tumour rather than back through the basolateral surface into the blood stream. |
| first_indexed | 2025-11-15T11:11:22Z |
| format | Article |
| id | upm-61987 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:11:22Z |
| publishDate | 2017 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-619872019-03-13T03:09:41Z http://psasir.upm.edu.my/id/eprint/61987/ Group B adenovirus enadenotucirev infects polarised colorectal cancer cells efficiently from the basolateral surface expected to be encountered during intravenous delivery to treat disseminated cancer Chia, Suet Lin Lei, Janet Ferguson, David J. P. Dyer, Arthur Fishera, Kerry D. Seymour, Leonard W. Enadenotucirev (EnAd) is a group B oncolytic adenovirus developed for systemic delivery and currently undergoing clinical evaluation for advanced cancer therapy. For differentiated carcinomas, systemic delivery would likely expose virus particles to the basolateral surface of cancer cells rather than the apical surface encountered during natural infection. Here, we compare the ability of EnAd and adenovirus type-5 (Ad5) to infect polarised colorectal carcinoma cells from the apical or basolateral surfaces. Whereas Ad5 infection was more efficient via the apical than basolateral surface, EnAd readily infected cells from either surface. Progeny particles from EnAd were released preferentially via the apical surface for all cell lines and routes of infection. These data further support the utility of group B adenoviruses for systemic delivery and suggest that progeny virus are more likely to be released into the tumour rather than back through the basolateral surface into the blood stream. Elsevier 2017-05 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/61987/1/Group%20B%20adenovirus%20enadenotucirev%20infects%20polarised%20colorectal%20cancer%20cells%20.pdf Chia, Suet Lin and Lei, Janet and Ferguson, David J. P. and Dyer, Arthur and Fishera, Kerry D. and Seymour, Leonard W. (2017) Group B adenovirus enadenotucirev infects polarised colorectal cancer cells efficiently from the basolateral surface expected to be encountered during intravenous delivery to treat disseminated cancer. Virology, 505. pp. 162-171. ISSN 0042-6822; ESSN: 1096-0341 https://www.sciencedirect.com/science/article/pii/S0042682217300521?via%3Dihub 10.1016/j.virol.2017.02.011 |
| spellingShingle | Chia, Suet Lin Lei, Janet Ferguson, David J. P. Dyer, Arthur Fishera, Kerry D. Seymour, Leonard W. Group B adenovirus enadenotucirev infects polarised colorectal cancer cells efficiently from the basolateral surface expected to be encountered during intravenous delivery to treat disseminated cancer |
| title | Group B adenovirus enadenotucirev infects polarised colorectal cancer cells efficiently from the basolateral surface expected to be encountered during intravenous delivery to treat disseminated cancer |
| title_full | Group B adenovirus enadenotucirev infects polarised colorectal cancer cells efficiently from the basolateral surface expected to be encountered during intravenous delivery to treat disseminated cancer |
| title_fullStr | Group B adenovirus enadenotucirev infects polarised colorectal cancer cells efficiently from the basolateral surface expected to be encountered during intravenous delivery to treat disseminated cancer |
| title_full_unstemmed | Group B adenovirus enadenotucirev infects polarised colorectal cancer cells efficiently from the basolateral surface expected to be encountered during intravenous delivery to treat disseminated cancer |
| title_short | Group B adenovirus enadenotucirev infects polarised colorectal cancer cells efficiently from the basolateral surface expected to be encountered during intravenous delivery to treat disseminated cancer |
| title_sort | group b adenovirus enadenotucirev infects polarised colorectal cancer cells efficiently from the basolateral surface expected to be encountered during intravenous delivery to treat disseminated cancer |
| url | http://psasir.upm.edu.my/id/eprint/61987/ http://psasir.upm.edu.my/id/eprint/61987/ http://psasir.upm.edu.my/id/eprint/61987/ http://psasir.upm.edu.my/id/eprint/61987/1/Group%20B%20adenovirus%20enadenotucirev%20infects%20polarised%20colorectal%20cancer%20cells%20.pdf |