Graphene oxide as a nanocarrier for controlled release and targeted delivery of an anticancer active agent, chlorogenic acid
We have synthesized graphene oxide using improved Hummer's method in order to explore the potential use of the resulting graphene oxide as a nanocarrier for an active anticancer agent, chlorogenic acid (CA). The synthesized graphene oxide and chlorogenic acid-graphene oxide nanocomposite (CAGO)...
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| Format: | Article |
| Language: | English |
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Elsevier
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/61973/ http://psasir.upm.edu.my/id/eprint/61973/1/Graphene%20oxide%20as%20a%20nanocarrier%20for%20controlled%20release%20.pdf |
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| author | Barahuie, Farahnaz Saifullah, Bullo Dorniani, Dena Fakurazi, Sharida Karthivashan, Govindarajan Hussein, Mohd Zobir Elfghi, Fawzi M. |
| author_facet | Barahuie, Farahnaz Saifullah, Bullo Dorniani, Dena Fakurazi, Sharida Karthivashan, Govindarajan Hussein, Mohd Zobir Elfghi, Fawzi M. |
| author_sort | Barahuie, Farahnaz |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | We have synthesized graphene oxide using improved Hummer's method in order to explore the potential use of the resulting graphene oxide as a nanocarrier for an active anticancer agent, chlorogenic acid (CA). The synthesized graphene oxide and chlorogenic acid-graphene oxide nanocomposite (CAGO) were characterized using Fourier transform infrared (FTIR) spectroscopy, thermogravimetry and differential thermogravimetry analysis, Raman spectroscopy, powder X-ray diffraction (PXRD), UV–vis spectroscopy and high resolution transmission electron microscopy (HRTEM) techniques. The successful conjugation of chlorogenic acid onto graphene oxide through hydrogen bonding and π–π interaction was confirmed by Raman spectroscopy, FTIR analysis and X-ray diffraction patterns. The loading of CA in the nanohybrid was estimated to be around 13.1% by UV–vis spectroscopy. The release profiles showed favourable, sustained and pH-dependent release of CA from CAGO nanocomposite and conformed well to the pseudo-second order kinetic model. Furthermore, the designed anticancer nanohybrid was thermally more stable than its counterpart. The in vitro cytotoxicity results revealed insignificant toxicity effect towards normal cell line, with a viability of > 80% even at higher concentration of 50 μg/mL. Contrarily, CAGO nanocomposite revealed enhanced toxic effect towards evaluated cancer cell lines (HepG2 human liver hepatocellular carcinoma cell line, A549 human lung adenocarcinoma epithelial cell line, and HeLa human cervical cancer cell line) compared to its free form. |
| first_indexed | 2025-11-15T11:11:19Z |
| format | Article |
| id | upm-61973 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:11:19Z |
| publishDate | 2017 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-619732019-03-11T09:00:41Z http://psasir.upm.edu.my/id/eprint/61973/ Graphene oxide as a nanocarrier for controlled release and targeted delivery of an anticancer active agent, chlorogenic acid Barahuie, Farahnaz Saifullah, Bullo Dorniani, Dena Fakurazi, Sharida Karthivashan, Govindarajan Hussein, Mohd Zobir Elfghi, Fawzi M. We have synthesized graphene oxide using improved Hummer's method in order to explore the potential use of the resulting graphene oxide as a nanocarrier for an active anticancer agent, chlorogenic acid (CA). The synthesized graphene oxide and chlorogenic acid-graphene oxide nanocomposite (CAGO) were characterized using Fourier transform infrared (FTIR) spectroscopy, thermogravimetry and differential thermogravimetry analysis, Raman spectroscopy, powder X-ray diffraction (PXRD), UV–vis spectroscopy and high resolution transmission electron microscopy (HRTEM) techniques. The successful conjugation of chlorogenic acid onto graphene oxide through hydrogen bonding and π–π interaction was confirmed by Raman spectroscopy, FTIR analysis and X-ray diffraction patterns. The loading of CA in the nanohybrid was estimated to be around 13.1% by UV–vis spectroscopy. The release profiles showed favourable, sustained and pH-dependent release of CA from CAGO nanocomposite and conformed well to the pseudo-second order kinetic model. Furthermore, the designed anticancer nanohybrid was thermally more stable than its counterpart. The in vitro cytotoxicity results revealed insignificant toxicity effect towards normal cell line, with a viability of > 80% even at higher concentration of 50 μg/mL. Contrarily, CAGO nanocomposite revealed enhanced toxic effect towards evaluated cancer cell lines (HepG2 human liver hepatocellular carcinoma cell line, A549 human lung adenocarcinoma epithelial cell line, and HeLa human cervical cancer cell line) compared to its free form. Elsevier 2017-05 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/61973/1/Graphene%20oxide%20as%20a%20nanocarrier%20for%20controlled%20release%20.pdf Barahuie, Farahnaz and Saifullah, Bullo and Dorniani, Dena and Fakurazi, Sharida and Karthivashan, Govindarajan and Hussein, Mohd Zobir and Elfghi, Fawzi M. (2017) Graphene oxide as a nanocarrier for controlled release and targeted delivery of an anticancer active agent, chlorogenic acid. Materials Science & Engineering C: Materials for Biological Applications, 74. 177 - 185. ISSN 0928-4931 https://www.sciencedirect.com/science/article/pii/S0928493116320008#! 10.1016/j.msec.2016.11.114 |
| spellingShingle | Barahuie, Farahnaz Saifullah, Bullo Dorniani, Dena Fakurazi, Sharida Karthivashan, Govindarajan Hussein, Mohd Zobir Elfghi, Fawzi M. Graphene oxide as a nanocarrier for controlled release and targeted delivery of an anticancer active agent, chlorogenic acid |
| title | Graphene oxide as a nanocarrier for controlled release and targeted delivery of an anticancer active agent, chlorogenic acid |
| title_full | Graphene oxide as a nanocarrier for controlled release and targeted delivery of an anticancer active agent, chlorogenic acid |
| title_fullStr | Graphene oxide as a nanocarrier for controlled release and targeted delivery of an anticancer active agent, chlorogenic acid |
| title_full_unstemmed | Graphene oxide as a nanocarrier for controlled release and targeted delivery of an anticancer active agent, chlorogenic acid |
| title_short | Graphene oxide as a nanocarrier for controlled release and targeted delivery of an anticancer active agent, chlorogenic acid |
| title_sort | graphene oxide as a nanocarrier for controlled release and targeted delivery of an anticancer active agent, chlorogenic acid |
| url | http://psasir.upm.edu.my/id/eprint/61973/ http://psasir.upm.edu.my/id/eprint/61973/ http://psasir.upm.edu.my/id/eprint/61973/ http://psasir.upm.edu.my/id/eprint/61973/1/Graphene%20oxide%20as%20a%20nanocarrier%20for%20controlled%20release%20.pdf |