GC-MS based metabolite profiling, antioxidant and antimicrobial properties of different solvent extracts of Malaysian Plectranthus amboinicus leaves
This study evaluates the phytochemistry, antioxidant, and antimicrobial effects of Plectranthus amboinicus leaves extracted in different solvents. The methanol extract contained the highest total phenolic ( mg GAE/g) and flavonoid contents ( mg RE/g) and exhibited the highest DPPH scavenging activit...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Hindawi Publishing Corporation
2017
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/61938/ http://psasir.upm.edu.my/id/eprint/61938/1/GC-MS%20based%20metabolite%20profiling.pdf |
| _version_ | 1848854521158041600 |
|---|---|
| author | Swamy, Mallappa Kumara Arumugam, Greetha Kaur, Ravinder Ghasemzadeh, Ali Mohd. Yusoff, Mazina Sinniah, Uma Rani |
| author_facet | Swamy, Mallappa Kumara Arumugam, Greetha Kaur, Ravinder Ghasemzadeh, Ali Mohd. Yusoff, Mazina Sinniah, Uma Rani |
| author_sort | Swamy, Mallappa Kumara |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | This study evaluates the phytochemistry, antioxidant, and antimicrobial effects of Plectranthus amboinicus leaves extracted in different solvents. The methanol extract contained the highest total phenolic ( mg GAE/g) and flavonoid contents ( mg RE/g) and exhibited the highest DPPH scavenging activity (90.13 ± 3.32%) followed by the acetone extract (80.23 ± 3.26%) at 500 μg/mL concentration. Similarly, the highest ferric ion reduction potential ( μM of Fe (II)/g dry weight) was exhibited by the methanol extract followed by the acetone extract ( μM of Fe (II)/g dry weight). The methanol extract showed greater antimicrobial activity against all the tested pathogens (Bacillus subtilis, Methicillin-resistant Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and Candida albicans). However, both hexane and acetone extracts failed to inhibit E. coli. S. aureus and C. albicans were more susceptible to all the extracts. Further, GC-MS analysis confirmed the occurrence of a total 46 phytocompounds in different solvent extracts. Some of the major compounds included carvacrol (37.7%), tetracontane (16.6%), squalene (15.6%), tetrapentacontane (13.7%), and Phytol (12.9%). In conclusion, extraction solvents influenced the recovery of phytocompounds and the highest pharmacological activities of the methanol extract could be correlated to the presence of additional bioactive compounds. |
| first_indexed | 2025-11-15T11:11:11Z |
| format | Article |
| id | upm-61938 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:11:11Z |
| publishDate | 2017 |
| publisher | Hindawi Publishing Corporation |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-619382019-03-04T04:31:44Z http://psasir.upm.edu.my/id/eprint/61938/ GC-MS based metabolite profiling, antioxidant and antimicrobial properties of different solvent extracts of Malaysian Plectranthus amboinicus leaves Swamy, Mallappa Kumara Arumugam, Greetha Kaur, Ravinder Ghasemzadeh, Ali Mohd. Yusoff, Mazina Sinniah, Uma Rani This study evaluates the phytochemistry, antioxidant, and antimicrobial effects of Plectranthus amboinicus leaves extracted in different solvents. The methanol extract contained the highest total phenolic ( mg GAE/g) and flavonoid contents ( mg RE/g) and exhibited the highest DPPH scavenging activity (90.13 ± 3.32%) followed by the acetone extract (80.23 ± 3.26%) at 500 μg/mL concentration. Similarly, the highest ferric ion reduction potential ( μM of Fe (II)/g dry weight) was exhibited by the methanol extract followed by the acetone extract ( μM of Fe (II)/g dry weight). The methanol extract showed greater antimicrobial activity against all the tested pathogens (Bacillus subtilis, Methicillin-resistant Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and Candida albicans). However, both hexane and acetone extracts failed to inhibit E. coli. S. aureus and C. albicans were more susceptible to all the extracts. Further, GC-MS analysis confirmed the occurrence of a total 46 phytocompounds in different solvent extracts. Some of the major compounds included carvacrol (37.7%), tetracontane (16.6%), squalene (15.6%), tetrapentacontane (13.7%), and Phytol (12.9%). In conclusion, extraction solvents influenced the recovery of phytocompounds and the highest pharmacological activities of the methanol extract could be correlated to the presence of additional bioactive compounds. Hindawi Publishing Corporation 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/61938/1/GC-MS%20based%20metabolite%20profiling.pdf Swamy, Mallappa Kumara and Arumugam, Greetha and Kaur, Ravinder and Ghasemzadeh, Ali and Mohd. Yusoff, Mazina and Sinniah, Uma Rani (2017) GC-MS based metabolite profiling, antioxidant and antimicrobial properties of different solvent extracts of Malaysian Plectranthus amboinicus leaves. Evidence-Based Complementary and Alternative Medicine, 2017. art. no. 1517683. pp. 1-10. ISSN 1741-427X; ESSN: 1741-4288 https://www.hindawi.com/journals/ecam/2017/1517683/ 10.1155/2017/1517683 |
| spellingShingle | Swamy, Mallappa Kumara Arumugam, Greetha Kaur, Ravinder Ghasemzadeh, Ali Mohd. Yusoff, Mazina Sinniah, Uma Rani GC-MS based metabolite profiling, antioxidant and antimicrobial properties of different solvent extracts of Malaysian Plectranthus amboinicus leaves |
| title | GC-MS based metabolite profiling, antioxidant and antimicrobial properties of different solvent extracts of Malaysian Plectranthus amboinicus leaves |
| title_full | GC-MS based metabolite profiling, antioxidant and antimicrobial properties of different solvent extracts of Malaysian Plectranthus amboinicus leaves |
| title_fullStr | GC-MS based metabolite profiling, antioxidant and antimicrobial properties of different solvent extracts of Malaysian Plectranthus amboinicus leaves |
| title_full_unstemmed | GC-MS based metabolite profiling, antioxidant and antimicrobial properties of different solvent extracts of Malaysian Plectranthus amboinicus leaves |
| title_short | GC-MS based metabolite profiling, antioxidant and antimicrobial properties of different solvent extracts of Malaysian Plectranthus amboinicus leaves |
| title_sort | gc-ms based metabolite profiling, antioxidant and antimicrobial properties of different solvent extracts of malaysian plectranthus amboinicus leaves |
| url | http://psasir.upm.edu.my/id/eprint/61938/ http://psasir.upm.edu.my/id/eprint/61938/ http://psasir.upm.edu.my/id/eprint/61938/ http://psasir.upm.edu.my/id/eprint/61938/1/GC-MS%20based%20metabolite%20profiling.pdf |