Factors affecting shoot and root apical meristem tissue culture of Thai supersweet corn (Zea mays var. rugosa)
During last recent years, in vitro propagation technic is widely used to produce plants with desirable traits. This experiment was conducted to produce an ideal protocol for in vitro propagation of Thai supersweet corn by using shoot apical meristem (SAM) and root apical meristem (RAM) as explants....
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/61846/ http://psasir.upm.edu.my/id/eprint/61846/1/Factors%20affecting%20shoot%20and%20root%20apical%20meristem%20tissue%20culture%20of%20Thai%20supersweet%20corn.pdf |
| _version_ | 1848854503271432192 |
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| author | Silvarajana, Lavanya Kalhori, Nahid Nulit, Rosimah Qamaruz Zaman, Faridah Rambod, Abiri Atabaki, Narges |
| author_facet | Silvarajana, Lavanya Kalhori, Nahid Nulit, Rosimah Qamaruz Zaman, Faridah Rambod, Abiri Atabaki, Narges |
| author_sort | Silvarajana, Lavanya |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | During last recent years, in vitro propagation technic is widely used to produce plants with desirable traits. This experiment was conducted to produce an ideal protocol for in vitro propagation of Thai supersweet corn by using shoot apical meristem (SAM) and root apical meristem (RAM) as explants. Four-day-old germinating seedlings were used as the experimental materials on culture media supplemented with a range of auxin, kinetin, and carbohydrates. The primary establishment for SAM showed the highest percentage of survival (80%) while RAM showed the highest survival (67%) and in Murashige and Skoog (MS) media supplemented. Upon acclimatization, regenerated plantlets from shoot showed the highest survival rate (12%) with the production of 21 plantlets; however, the survival rate of plantlets from root was only 20% with the production of 9 plantlets. The efficient and economic protocol that is produced in this study can be applied as an alternative to conventional propagation method for the large-scale production of Thai supersweet corn throughout the year. |
| first_indexed | 2025-11-15T11:10:54Z |
| format | Article |
| id | upm-61846 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:10:54Z |
| publishDate | 2017 |
| publisher | Taylor & Francis Group |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-618462019-02-20T09:10:00Z http://psasir.upm.edu.my/id/eprint/61846/ Factors affecting shoot and root apical meristem tissue culture of Thai supersweet corn (Zea mays var. rugosa) Silvarajana, Lavanya Kalhori, Nahid Nulit, Rosimah Qamaruz Zaman, Faridah Rambod, Abiri Atabaki, Narges During last recent years, in vitro propagation technic is widely used to produce plants with desirable traits. This experiment was conducted to produce an ideal protocol for in vitro propagation of Thai supersweet corn by using shoot apical meristem (SAM) and root apical meristem (RAM) as explants. Four-day-old germinating seedlings were used as the experimental materials on culture media supplemented with a range of auxin, kinetin, and carbohydrates. The primary establishment for SAM showed the highest percentage of survival (80%) while RAM showed the highest survival (67%) and in Murashige and Skoog (MS) media supplemented. Upon acclimatization, regenerated plantlets from shoot showed the highest survival rate (12%) with the production of 21 plantlets; however, the survival rate of plantlets from root was only 20% with the production of 9 plantlets. The efficient and economic protocol that is produced in this study can be applied as an alternative to conventional propagation method for the large-scale production of Thai supersweet corn throughout the year. Taylor & Francis Group 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/61846/1/Factors%20affecting%20shoot%20and%20root%20apical%20meristem%20tissue%20culture%20of%20Thai%20supersweet%20corn.pdf Silvarajana, Lavanya and Kalhori, Nahid and Nulit, Rosimah and Qamaruz Zaman, Faridah and Rambod, Abiri and Atabaki, Narges (2017) Factors affecting shoot and root apical meristem tissue culture of Thai supersweet corn (Zea mays var. rugosa). Journal of Plant Nutrition, 40 (12). 1677 - 1689. ISSN 0190-4167; ESSN: 1532-4087 https://www.tandfonline.com/doi/abs/10.1080/01904167.2017.1310889 10.1080/01904167.2017.1310889 |
| spellingShingle | Silvarajana, Lavanya Kalhori, Nahid Nulit, Rosimah Qamaruz Zaman, Faridah Rambod, Abiri Atabaki, Narges Factors affecting shoot and root apical meristem tissue culture of Thai supersweet corn (Zea mays var. rugosa) |
| title | Factors affecting shoot and root apical meristem tissue culture of Thai supersweet corn (Zea mays var. rugosa) |
| title_full | Factors affecting shoot and root apical meristem tissue culture of Thai supersweet corn (Zea mays var. rugosa) |
| title_fullStr | Factors affecting shoot and root apical meristem tissue culture of Thai supersweet corn (Zea mays var. rugosa) |
| title_full_unstemmed | Factors affecting shoot and root apical meristem tissue culture of Thai supersweet corn (Zea mays var. rugosa) |
| title_short | Factors affecting shoot and root apical meristem tissue culture of Thai supersweet corn (Zea mays var. rugosa) |
| title_sort | factors affecting shoot and root apical meristem tissue culture of thai supersweet corn (zea mays var. rugosa) |
| url | http://psasir.upm.edu.my/id/eprint/61846/ http://psasir.upm.edu.my/id/eprint/61846/ http://psasir.upm.edu.my/id/eprint/61846/ http://psasir.upm.edu.my/id/eprint/61846/1/Factors%20affecting%20shoot%20and%20root%20apical%20meristem%20tissue%20culture%20of%20Thai%20supersweet%20corn.pdf |