Efficiency of newly formulated camptothecin with β-cyclodextrin-EDTA-Fe3O4 nanoparticle-conjugated nanocarriers as an anti-colon cancer (HT29) drug
Camptothecin (CPT) is an anti-cancer drug that effectively treats various cancers, including colon cancer. However, poor solubility and other drawbacks have restricted its chemotherapeutic potential. To overcome these restrictions, CPT was encapsulated in CEF (cyclodextrin-EDTA-FE3O4), a composite n...
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| Format: | Article |
| Language: | English |
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Nature Publishing Group
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/61681/ http://psasir.upm.edu.my/id/eprint/61681/1/Efciency%20of%20newly%20formulated.pdf |
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| author | Krishnan, Poorani Rajan, Mariappan Kumari, Sharmilah Syed, Sakinah Priya, Sivan Padma Fatin Amira Danjuma, Lawal Mok, Pooi Ling Fakurazi, Sharida Arulselvan, Palanisamy Higuchi, Akon Arumugam, Ramitha Alarfaj, Abdullah A. Munusamy, Murugan A. Hamat, Rukman Awang Benelli, Giovanni Murugan, Kadarkarai Kumar, S. Suresh |
| author_facet | Krishnan, Poorani Rajan, Mariappan Kumari, Sharmilah Syed, Sakinah Priya, Sivan Padma Fatin Amira Danjuma, Lawal Mok, Pooi Ling Fakurazi, Sharida Arulselvan, Palanisamy Higuchi, Akon Arumugam, Ramitha Alarfaj, Abdullah A. Munusamy, Murugan A. Hamat, Rukman Awang Benelli, Giovanni Murugan, Kadarkarai Kumar, S. Suresh |
| author_sort | Krishnan, Poorani |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Camptothecin (CPT) is an anti-cancer drug that effectively treats various cancers, including colon cancer. However, poor solubility and other drawbacks have restricted its chemotherapeutic potential. To overcome these restrictions, CPT was encapsulated in CEF (cyclodextrin-EDTA-FE3O4), a composite nanoparticle of magnetic iron oxide (Fe3O4), and β-cyclodextrin was cross-linked with ethylenediaminetetraacetic acid (EDTA). This formulation improved CPT's solubility and bioavailability for cancer cells. The use of magnetically responsive anti-cancer formulation is highly advantageous in cancer chemotherapy. The chemical characterisation of CPT-CEF was studied here. The ability of this nano-compound to induce apoptosis in HT29 colon cancer cells and A549 lung cancer cells was evaluated. The dose-dependent cytotoxicity of CPT-CEF was shown using MTT. Propidium iodide and Annexin V staining, mitochondrial membrane depolarisation (JC-1 dye), and caspase-3 activity were assayed to detect apoptosis in CPT-CEF-treated cancer cells. Cell cycle analysis also showed G1 phase arrest, which indicated possible synergistic effects of the nano-carrier. These study results show that CPT-CEF causes a dose-dependent cell viability reduction in HT29 and A549 cells and induces apoptosis in colon cancer cells via caspase-3 activation. These data strongly suggest that CPT could be used as a major nanocarrier for CPT to effectively treat colon cancer. |
| first_indexed | 2025-11-15T11:10:17Z |
| format | Article |
| id | upm-61681 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:10:17Z |
| publishDate | 2017 |
| publisher | Nature Publishing Group |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-616812018-10-31T07:02:29Z http://psasir.upm.edu.my/id/eprint/61681/ Efficiency of newly formulated camptothecin with β-cyclodextrin-EDTA-Fe3O4 nanoparticle-conjugated nanocarriers as an anti-colon cancer (HT29) drug Krishnan, Poorani Rajan, Mariappan Kumari, Sharmilah Syed, Sakinah Priya, Sivan Padma Fatin Amira Danjuma, Lawal Mok, Pooi Ling Fakurazi, Sharida Arulselvan, Palanisamy Higuchi, Akon Arumugam, Ramitha Alarfaj, Abdullah A. Munusamy, Murugan A. Hamat, Rukman Awang Benelli, Giovanni Murugan, Kadarkarai Kumar, S. Suresh Camptothecin (CPT) is an anti-cancer drug that effectively treats various cancers, including colon cancer. However, poor solubility and other drawbacks have restricted its chemotherapeutic potential. To overcome these restrictions, CPT was encapsulated in CEF (cyclodextrin-EDTA-FE3O4), a composite nanoparticle of magnetic iron oxide (Fe3O4), and β-cyclodextrin was cross-linked with ethylenediaminetetraacetic acid (EDTA). This formulation improved CPT's solubility and bioavailability for cancer cells. The use of magnetically responsive anti-cancer formulation is highly advantageous in cancer chemotherapy. The chemical characterisation of CPT-CEF was studied here. The ability of this nano-compound to induce apoptosis in HT29 colon cancer cells and A549 lung cancer cells was evaluated. The dose-dependent cytotoxicity of CPT-CEF was shown using MTT. Propidium iodide and Annexin V staining, mitochondrial membrane depolarisation (JC-1 dye), and caspase-3 activity were assayed to detect apoptosis in CPT-CEF-treated cancer cells. Cell cycle analysis also showed G1 phase arrest, which indicated possible synergistic effects of the nano-carrier. These study results show that CPT-CEF causes a dose-dependent cell viability reduction in HT29 and A549 cells and induces apoptosis in colon cancer cells via caspase-3 activation. These data strongly suggest that CPT could be used as a major nanocarrier for CPT to effectively treat colon cancer. Nature Publishing Group 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/61681/1/Efciency%20of%20newly%20formulated.pdf Krishnan, Poorani and Rajan, Mariappan and Kumari, Sharmilah and Syed, Sakinah and Priya, Sivan Padma and Fatin Amira and Danjuma, Lawal and Mok, Pooi Ling and Fakurazi, Sharida and Arulselvan, Palanisamy and Higuchi, Akon and Arumugam, Ramitha and Alarfaj, Abdullah A. and Munusamy, Murugan A. and Hamat, Rukman Awang and Benelli, Giovanni and Murugan, Kadarkarai and Kumar, S. Suresh (2017) Efficiency of newly formulated camptothecin with β-cyclodextrin-EDTA-Fe3O4 nanoparticle-conjugated nanocarriers as an anti-colon cancer (HT29) drug. Scientific Reports, 7 (1). pp. 1-16. ISSN 2045-2322 10.1038/s41598-017-09140-1 |
| spellingShingle | Krishnan, Poorani Rajan, Mariappan Kumari, Sharmilah Syed, Sakinah Priya, Sivan Padma Fatin Amira Danjuma, Lawal Mok, Pooi Ling Fakurazi, Sharida Arulselvan, Palanisamy Higuchi, Akon Arumugam, Ramitha Alarfaj, Abdullah A. Munusamy, Murugan A. Hamat, Rukman Awang Benelli, Giovanni Murugan, Kadarkarai Kumar, S. Suresh Efficiency of newly formulated camptothecin with β-cyclodextrin-EDTA-Fe3O4 nanoparticle-conjugated nanocarriers as an anti-colon cancer (HT29) drug |
| title | Efficiency of newly formulated camptothecin with β-cyclodextrin-EDTA-Fe3O4 nanoparticle-conjugated nanocarriers as an anti-colon cancer (HT29) drug |
| title_full | Efficiency of newly formulated camptothecin with β-cyclodextrin-EDTA-Fe3O4 nanoparticle-conjugated nanocarriers as an anti-colon cancer (HT29) drug |
| title_fullStr | Efficiency of newly formulated camptothecin with β-cyclodextrin-EDTA-Fe3O4 nanoparticle-conjugated nanocarriers as an anti-colon cancer (HT29) drug |
| title_full_unstemmed | Efficiency of newly formulated camptothecin with β-cyclodextrin-EDTA-Fe3O4 nanoparticle-conjugated nanocarriers as an anti-colon cancer (HT29) drug |
| title_short | Efficiency of newly formulated camptothecin with β-cyclodextrin-EDTA-Fe3O4 nanoparticle-conjugated nanocarriers as an anti-colon cancer (HT29) drug |
| title_sort | efficiency of newly formulated camptothecin with β-cyclodextrin-edta-fe3o4 nanoparticle-conjugated nanocarriers as an anti-colon cancer (ht29) drug |
| url | http://psasir.upm.edu.my/id/eprint/61681/ http://psasir.upm.edu.my/id/eprint/61681/ http://psasir.upm.edu.my/id/eprint/61681/1/Efciency%20of%20newly%20formulated.pdf |