Effects of micronutrients (Cu, Zn, Mn, and Fe) on the growth of Spathoglottis plicata plantlets
The micropropagation of valuable orchid species such as Spathoglottis plicata could help in their conservation and increase their propagation rate. The objective of this study was to investigate the effects of micronutrients (CuSO4, ZnSO4, MnSO4 and Fe-EDTA) on the growth of S. plicata plantlets. Pl...
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| Language: | English |
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Termedia Publishing House
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/61659/ http://psasir.upm.edu.my/id/eprint/61659/1/Effects%20of%20micronutrients%20.pdf |
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| author | Mat Yasin, Zaliyatun Akhma Mahmood, Maziah Shaharudiin, Noor Azmi |
| author_facet | Mat Yasin, Zaliyatun Akhma Mahmood, Maziah Shaharudiin, Noor Azmi |
| author_sort | Mat Yasin, Zaliyatun Akhma |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The micropropagation of valuable orchid species such as Spathoglottis plicata could help in their conservation and increase their propagation rate. The objective of this study was to investigate the effects of micronutrients (CuSO4, ZnSO4, MnSO4 and Fe-EDTA) on the growth of S. plicata plantlets. Plantlets of uniform height (1.5 cm) were transferred to a half-strength MS media supplemented with vitamin B5 and different concentrations of selected micronutrients (copper, zinc, manganese and iron). The highest production of soluble protein content (38.98 mg/g of fresh weight, FW) was recorded when plantlets were treated with 25 μM MnSO4. Spathoglottis plicata plantlets formed the highest amount of chlorophyll (22.32 mg/g FW) when the growth media were supplemented with 75 μM Fe-EDTA. A total of 25 μM Fe-EDTA induced the production of up to 19.78 mg/g FW of carbohydrates in S. plicata plantlets. Furthermore, we demonstrate that different concentrations of micronutrients had different effects on the activities of several enzymes, such as peroxidase, catalase, polyphenol oxidase and nitrate reductase. |
| first_indexed | 2025-11-15T11:10:12Z |
| format | Article |
| id | upm-61659 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:10:12Z |
| publishDate | 2017 |
| publisher | Termedia Publishing House |
| recordtype | eprints |
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| spelling | upm-616592018-10-30T02:49:50Z http://psasir.upm.edu.my/id/eprint/61659/ Effects of micronutrients (Cu, Zn, Mn, and Fe) on the growth of Spathoglottis plicata plantlets Mat Yasin, Zaliyatun Akhma Mahmood, Maziah Shaharudiin, Noor Azmi The micropropagation of valuable orchid species such as Spathoglottis plicata could help in their conservation and increase their propagation rate. The objective of this study was to investigate the effects of micronutrients (CuSO4, ZnSO4, MnSO4 and Fe-EDTA) on the growth of S. plicata plantlets. Plantlets of uniform height (1.5 cm) were transferred to a half-strength MS media supplemented with vitamin B5 and different concentrations of selected micronutrients (copper, zinc, manganese and iron). The highest production of soluble protein content (38.98 mg/g of fresh weight, FW) was recorded when plantlets were treated with 25 μM MnSO4. Spathoglottis plicata plantlets formed the highest amount of chlorophyll (22.32 mg/g FW) when the growth media were supplemented with 75 μM Fe-EDTA. A total of 25 μM Fe-EDTA induced the production of up to 19.78 mg/g FW of carbohydrates in S. plicata plantlets. Furthermore, we demonstrate that different concentrations of micronutrients had different effects on the activities of several enzymes, such as peroxidase, catalase, polyphenol oxidase and nitrate reductase. Termedia Publishing House 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/61659/1/Effects%20of%20micronutrients%20.pdf Mat Yasin, Zaliyatun Akhma and Mahmood, Maziah and Shaharudiin, Noor Azmi (2017) Effects of micronutrients (Cu, Zn, Mn, and Fe) on the growth of Spathoglottis plicata plantlets. Biotechnology, Computational Biology and Bionanotechnology, 98 (1). pp. 5-13. ISSN 0860-7796; ESSN2353-9461 http://www.biotechnologia-journal.org/Effects-of-micronutrients-Cu-Zn-Mn-and-Fe-on-the-growth-of-Spathoglottis-plicata-plantlets,85,29641,0,1.html 10.5114/bta.2017.66612 |
| spellingShingle | Mat Yasin, Zaliyatun Akhma Mahmood, Maziah Shaharudiin, Noor Azmi Effects of micronutrients (Cu, Zn, Mn, and Fe) on the growth of Spathoglottis plicata plantlets |
| title | Effects of micronutrients (Cu, Zn, Mn, and Fe) on the growth of Spathoglottis plicata plantlets |
| title_full | Effects of micronutrients (Cu, Zn, Mn, and Fe) on the growth of Spathoglottis plicata plantlets |
| title_fullStr | Effects of micronutrients (Cu, Zn, Mn, and Fe) on the growth of Spathoglottis plicata plantlets |
| title_full_unstemmed | Effects of micronutrients (Cu, Zn, Mn, and Fe) on the growth of Spathoglottis plicata plantlets |
| title_short | Effects of micronutrients (Cu, Zn, Mn, and Fe) on the growth of Spathoglottis plicata plantlets |
| title_sort | effects of micronutrients (cu, zn, mn, and fe) on the growth of spathoglottis plicata plantlets |
| url | http://psasir.upm.edu.my/id/eprint/61659/ http://psasir.upm.edu.my/id/eprint/61659/ http://psasir.upm.edu.my/id/eprint/61659/ http://psasir.upm.edu.my/id/eprint/61659/1/Effects%20of%20micronutrients%20.pdf |