Effect of Nigella sativa pre-treatment on sub-chronic lead acetate induced hematological and biochemical alterations
Lead acetate (LA) toxicity can occur either by ingestion or inhalation from contaminated surfaces or from the environment. Nigella sativa is a natural product with immense pharmacological properties. In this study, the effects of N. sativa pre-treatment on lead acetate induced hematological and bioc...
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| Format: | Article |
| Language: | English |
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American Scientific Publishers
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/61584/ http://psasir.upm.edu.my/id/eprint/61584/1/Effect%20of%20Nigella%20sativa%20administration%20on%20sub-chronic%20lead%20acetate%20induced%20haematological%20and%20biochemical%20alterations_new.pdf |
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| author | M. A, Mohd Assi Mohd Noor, M. Hezmee Abba, Yusuf Mohd Yusoff, Md Sabri Haron, Abd Wahid H., Falah Baiee Rajion, Mohd Ali |
| author_facet | M. A, Mohd Assi Mohd Noor, M. Hezmee Abba, Yusuf Mohd Yusoff, Md Sabri Haron, Abd Wahid H., Falah Baiee Rajion, Mohd Ali |
| author_sort | M. A, Mohd Assi |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Lead acetate (LA) toxicity can occur either by ingestion or inhalation from contaminated surfaces or from the environment. Nigella sativa is a natural product with immense pharmacological properties. In this study, the effects of N. sativa pre-treatment on lead acetate induced hematological and biochemical changes were evaluated. A total of 20 male Sprague Dawley rats were divided into 4 groups with 5 rats each. Group 1 (NC) was the negative control, group 2 was the lead acetate control (PC) and was administered 10 mg/kg/per day of lead acetate (LA) per OS for 30 days, group 3 (T1) was administered 200 mg/kg/daily of Nigella sativa orally for a month and Group 4 (T2) was pre-treated with 200 mg/kg/daily of Nigella sativa orally for one month, followed by administration of 10 mg/kg/daily of lead acetate (LA) orally for another month. At the end of the experiment, whole blood and serum were collected to evaluate the complete blood profile and serum biochemistry. The haemogram showed lower (p < 0.05) level of hemoglobin, packed cell volume and prothrombin in the PC group, while total white blood cell count, band neutrophils, segmented neutrophils, lymphocytes and monocytes counts were higher (p < 0.05) in the PC group than the treatment groups. However, eosinophil count was higher in T2, while no changes were observed in RBC and MCV values. Both alanine and aspartate aminotransferase enzymes were higher in the PC as compared to other groups. Similarly, the levels of alkaline phosphatase, cholesterol, urea and creatinine were all higher (p < 0.05) in the PC group and comparable (p > 0.05) in the control, T1 and T2 groups. The level of SOD and GSH were lower (p < 0.05) in the PC and T2 groups. In summary, this study showed the prophylactic potential of N. sativa extract in modulating both hematological, biochemical and anti-oxidant enzymes alterations induced by sub-chronic lead acetate administration in rats. |
| first_indexed | 2025-11-15T11:09:55Z |
| format | Article |
| id | upm-61584 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:09:55Z |
| publishDate | 2017 |
| publisher | American Scientific Publishers |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-615842022-07-07T02:04:52Z http://psasir.upm.edu.my/id/eprint/61584/ Effect of Nigella sativa pre-treatment on sub-chronic lead acetate induced hematological and biochemical alterations M. A, Mohd Assi Mohd Noor, M. Hezmee Abba, Yusuf Mohd Yusoff, Md Sabri Haron, Abd Wahid H., Falah Baiee Rajion, Mohd Ali Lead acetate (LA) toxicity can occur either by ingestion or inhalation from contaminated surfaces or from the environment. Nigella sativa is a natural product with immense pharmacological properties. In this study, the effects of N. sativa pre-treatment on lead acetate induced hematological and biochemical changes were evaluated. A total of 20 male Sprague Dawley rats were divided into 4 groups with 5 rats each. Group 1 (NC) was the negative control, group 2 was the lead acetate control (PC) and was administered 10 mg/kg/per day of lead acetate (LA) per OS for 30 days, group 3 (T1) was administered 200 mg/kg/daily of Nigella sativa orally for a month and Group 4 (T2) was pre-treated with 200 mg/kg/daily of Nigella sativa orally for one month, followed by administration of 10 mg/kg/daily of lead acetate (LA) orally for another month. At the end of the experiment, whole blood and serum were collected to evaluate the complete blood profile and serum biochemistry. The haemogram showed lower (p < 0.05) level of hemoglobin, packed cell volume and prothrombin in the PC group, while total white blood cell count, band neutrophils, segmented neutrophils, lymphocytes and monocytes counts were higher (p < 0.05) in the PC group than the treatment groups. However, eosinophil count was higher in T2, while no changes were observed in RBC and MCV values. Both alanine and aspartate aminotransferase enzymes were higher in the PC as compared to other groups. Similarly, the levels of alkaline phosphatase, cholesterol, urea and creatinine were all higher (p < 0.05) in the PC group and comparable (p > 0.05) in the control, T1 and T2 groups. The level of SOD and GSH were lower (p < 0.05) in the PC and T2 groups. In summary, this study showed the prophylactic potential of N. sativa extract in modulating both hematological, biochemical and anti-oxidant enzymes alterations induced by sub-chronic lead acetate administration in rats. American Scientific Publishers 2017-06 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/61584/1/Effect%20of%20Nigella%20sativa%20administration%20on%20sub-chronic%20lead%20acetate%20induced%20haematological%20and%20biochemical%20alterations_new.pdf M. A, Mohd Assi and Mohd Noor, M. Hezmee and Abba, Yusuf and Mohd Yusoff, Md Sabri and Haron, Abd Wahid and H., Falah Baiee and Rajion, Mohd Ali (2017) Effect of Nigella sativa pre-treatment on sub-chronic lead acetate induced hematological and biochemical alterations. Journal of Computational and Theoretical Nanoscience, 14 (6). 2752 - 2758. ISSN 1546-1955; ESSN: 1546-1963 https://www.ingentaconnect.com/content/asp/jctn/2017/00000014/00000006/art00022 10.1007/s00580-018-2655-z |
| spellingShingle | M. A, Mohd Assi Mohd Noor, M. Hezmee Abba, Yusuf Mohd Yusoff, Md Sabri Haron, Abd Wahid H., Falah Baiee Rajion, Mohd Ali Effect of Nigella sativa pre-treatment on sub-chronic lead acetate induced hematological and biochemical alterations |
| title | Effect of Nigella sativa pre-treatment on sub-chronic lead acetate induced hematological and biochemical alterations |
| title_full | Effect of Nigella sativa pre-treatment on sub-chronic lead acetate induced hematological and biochemical alterations |
| title_fullStr | Effect of Nigella sativa pre-treatment on sub-chronic lead acetate induced hematological and biochemical alterations |
| title_full_unstemmed | Effect of Nigella sativa pre-treatment on sub-chronic lead acetate induced hematological and biochemical alterations |
| title_short | Effect of Nigella sativa pre-treatment on sub-chronic lead acetate induced hematological and biochemical alterations |
| title_sort | effect of nigella sativa pre-treatment on sub-chronic lead acetate induced hematological and biochemical alterations |
| url | http://psasir.upm.edu.my/id/eprint/61584/ http://psasir.upm.edu.my/id/eprint/61584/ http://psasir.upm.edu.my/id/eprint/61584/ http://psasir.upm.edu.my/id/eprint/61584/1/Effect%20of%20Nigella%20sativa%20administration%20on%20sub-chronic%20lead%20acetate%20induced%20haematological%20and%20biochemical%20alterations_new.pdf |