Effect of encapsulant and cryoprotectant on the viability of probiotic Pediococcus acidilactici ATCC 8042 during freeze-drying and exposure to high acidity, bile salts and heat
Encapsulation and freeze drying are among the most preferable preservation methods due to their considerable effects on probiotics survival. The effects of different cryoprotectants on the viability of free cells during freeze drying and coating materials for encapsulation strategy have been investi...
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| Format: | Article |
| Language: | English |
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Elsevier
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/61565/ http://psasir.upm.edu.my/id/eprint/61565/1/Effect%20of%20encapsulant%20and%20cryoprotectant%20on%20the%20viability%20of%20probiotic%20Pediococcus%20acidilactici%20ATCC%208042%20during%20freeze-drying%20and%20exposure%20to%20high%20acidity%2C%20bile%20salts%20and%20heat.pdf |
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| author | Halim, Murni Mohd Mustafa, Nur Anizawanie Othman, Majdiah Wasoh, Helmi Kapri, Mohd Rizal Ariff, Arbakariya |
| author_facet | Halim, Murni Mohd Mustafa, Nur Anizawanie Othman, Majdiah Wasoh, Helmi Kapri, Mohd Rizal Ariff, Arbakariya |
| author_sort | Halim, Murni |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Encapsulation and freeze drying are among the most preferable preservation methods due to their considerable effects on probiotics survival. The effects of different cryoprotectants on the viability of free cells during freeze drying and coating materials for encapsulation strategy have been investigated by various researchers. However, information on the effect of cryoprotectants on the viability of the encapsulated probiotics during freeze-drying seems scarce. In this study Pediococcus acidilactici ATCC 8042 was encapsulated using calcium-alginate (ALGsm-FD) and calcium-alginate co-coating with chitosan (CHsm-FD) via extrusion technique. They were subsequently freeze dried using 10% (w/w) skim milk as cryoprotectant. Immediately after the freeze drying, ALGsm-FD showed the highest survival (15.77%) compared to the CHsm-FD (0.36%), free cells protected with skim milk (FCsm-FD) (11.02%) and both encapsulated and free cell samples without the addition of skim milk (0%). Co-coating the entrapped cells in alginate bead with chitosan was only accommodating the cells survival during bile solution (pH ¼ 8.25) treatment but not for freeze drying and gastric juice treatment (pH ¼ 1.55). In comparison, ALGsm-FD and CHsm-FD were found to be more heat tolerance than FCsm-FD. The findings indicated that the supplementation of cryoprotectant conferred an important protective effect to either encapsulated or free probiotic cells during freeze drying. |
| first_indexed | 2025-11-15T11:09:51Z |
| format | Article |
| id | upm-61565 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:09:51Z |
| publishDate | 2017 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-615652022-05-25T02:12:06Z http://psasir.upm.edu.my/id/eprint/61565/ Effect of encapsulant and cryoprotectant on the viability of probiotic Pediococcus acidilactici ATCC 8042 during freeze-drying and exposure to high acidity, bile salts and heat Halim, Murni Mohd Mustafa, Nur Anizawanie Othman, Majdiah Wasoh, Helmi Kapri, Mohd Rizal Ariff, Arbakariya Encapsulation and freeze drying are among the most preferable preservation methods due to their considerable effects on probiotics survival. The effects of different cryoprotectants on the viability of free cells during freeze drying and coating materials for encapsulation strategy have been investigated by various researchers. However, information on the effect of cryoprotectants on the viability of the encapsulated probiotics during freeze-drying seems scarce. In this study Pediococcus acidilactici ATCC 8042 was encapsulated using calcium-alginate (ALGsm-FD) and calcium-alginate co-coating with chitosan (CHsm-FD) via extrusion technique. They were subsequently freeze dried using 10% (w/w) skim milk as cryoprotectant. Immediately after the freeze drying, ALGsm-FD showed the highest survival (15.77%) compared to the CHsm-FD (0.36%), free cells protected with skim milk (FCsm-FD) (11.02%) and both encapsulated and free cell samples without the addition of skim milk (0%). Co-coating the entrapped cells in alginate bead with chitosan was only accommodating the cells survival during bile solution (pH ¼ 8.25) treatment but not for freeze drying and gastric juice treatment (pH ¼ 1.55). In comparison, ALGsm-FD and CHsm-FD were found to be more heat tolerance than FCsm-FD. The findings indicated that the supplementation of cryoprotectant conferred an important protective effect to either encapsulated or free probiotic cells during freeze drying. Elsevier 2017-08 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/61565/1/Effect%20of%20encapsulant%20and%20cryoprotectant%20on%20the%20viability%20of%20probiotic%20Pediococcus%20acidilactici%20ATCC%208042%20during%20freeze-drying%20and%20exposure%20to%20high%20acidity%2C%20bile%20salts%20and%20heat.pdf Halim, Murni and Mohd Mustafa, Nur Anizawanie and Othman, Majdiah and Wasoh, Helmi and Kapri, Mohd Rizal and Ariff, Arbakariya (2017) Effect of encapsulant and cryoprotectant on the viability of probiotic Pediococcus acidilactici ATCC 8042 during freeze-drying and exposure to high acidity, bile salts and heat. LWT- Food Science and Technology, 81. 210 - 216. ISSN 0023-6438 https://www.sciencedirect.com/science/article/pii/S0023643817302232?via%3Dihub 10.1016/j.lwt.2017.04.009 |
| spellingShingle | Halim, Murni Mohd Mustafa, Nur Anizawanie Othman, Majdiah Wasoh, Helmi Kapri, Mohd Rizal Ariff, Arbakariya Effect of encapsulant and cryoprotectant on the viability of probiotic Pediococcus acidilactici ATCC 8042 during freeze-drying and exposure to high acidity, bile salts and heat |
| title | Effect of encapsulant and cryoprotectant on the viability of probiotic Pediococcus acidilactici ATCC 8042 during freeze-drying and exposure to high acidity, bile salts and heat |
| title_full | Effect of encapsulant and cryoprotectant on the viability of probiotic Pediococcus acidilactici ATCC 8042 during freeze-drying and exposure to high acidity, bile salts and heat |
| title_fullStr | Effect of encapsulant and cryoprotectant on the viability of probiotic Pediococcus acidilactici ATCC 8042 during freeze-drying and exposure to high acidity, bile salts and heat |
| title_full_unstemmed | Effect of encapsulant and cryoprotectant on the viability of probiotic Pediococcus acidilactici ATCC 8042 during freeze-drying and exposure to high acidity, bile salts and heat |
| title_short | Effect of encapsulant and cryoprotectant on the viability of probiotic Pediococcus acidilactici ATCC 8042 during freeze-drying and exposure to high acidity, bile salts and heat |
| title_sort | effect of encapsulant and cryoprotectant on the viability of probiotic pediococcus acidilactici atcc 8042 during freeze-drying and exposure to high acidity, bile salts and heat |
| url | http://psasir.upm.edu.my/id/eprint/61565/ http://psasir.upm.edu.my/id/eprint/61565/ http://psasir.upm.edu.my/id/eprint/61565/ http://psasir.upm.edu.my/id/eprint/61565/1/Effect%20of%20encapsulant%20and%20cryoprotectant%20on%20the%20viability%20of%20probiotic%20Pediococcus%20acidilactici%20ATCC%208042%20during%20freeze-drying%20and%20exposure%20to%20high%20acidity%2C%20bile%20salts%20and%20heat.pdf |