Different porcine reproductive and respiratory syndrome (PRRS) vaccine regimes and its effect on pig immunity status at Southeast Asia pig farms

Porcine reproductive and respiratory syndrome (PRRS) is a disease characterised by late-term reproductive failure in sows and gilts, and respiratory problems in piglets and growing pigs. In this study, 240 sera were collected from four farms that had been practicing different PRRS vaccination regime...

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Main Authors: Cheah, Z. H., Ooi, Peck Toung, Phang, Lai Yee, Chua, V., Low, S. E., Cheah, H. C., Lim, E., Yong, C. K., Kam, K. Y.
Format: Article
Language:English
Published: Malaysian Society of Parasitology and Tropical Medicine 2017
Online Access:http://psasir.upm.edu.my/id/eprint/61477/
http://psasir.upm.edu.my/id/eprint/61477/1/Different%20porcine%20reproductive%20and%20respiratory%20syndrome%20%28PRRS%29%20vaccine%20regimes%20and%20its%20effect%20on%20pig%20immunity%20status%20at%20Southeast%20Asia%20pig%20farms.pdf
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author Cheah, Z. H.
Ooi, Peck Toung
Phang, Lai Yee
Chua, V.
Low, S. E.
Cheah, H. C.
Lim, E.
Yong, C. K.
Kam, K. Y.
author_facet Cheah, Z. H.
Ooi, Peck Toung
Phang, Lai Yee
Chua, V.
Low, S. E.
Cheah, H. C.
Lim, E.
Yong, C. K.
Kam, K. Y.
author_sort Cheah, Z. H.
building UPM Institutional Repository
collection Online Access
description Porcine reproductive and respiratory syndrome (PRRS) is a disease characterised by late-term reproductive failure in sows and gilts, and respiratory problems in piglets and growing pigs. In this study, 240 sera were collected from four farms that had been practicing different PRRS vaccination regime for more than a year and vaccinations were done at 2 months before sampling. Fifteen sera samples from four age groups: sows, growers, weaners and piglets were collected from each farm and analysed using IDEXX PRRS X3 ELISA for PRRSV antibodies. Pooled serum samples were tested by using nested-PCR that enable the differentiation of Type I and Type II PRRSV. Out of 80 pooled serum samples, none were positive for PRRSV indicating all age groups were not viraemic after vaccination. Results by ELISA test showed all the farms were seropositive for PRRS. ELISA testing showed no significant difference between the farms except for Farm B which practised whole herd US MLV vaccination. Farm B showed significantly lower (p< 0.05) S/P ratio in their piglet, grower and sow groups which suggest there was low virus circulation in herd. Farm A which practised US MLV on sow was the only farm found to have seronegative status in their weaners. Data indicates PRRS MLV vaccination will not cause viraemia post four weeks vaccination and whole herd MLV vaccination may help to reduce virus circulation in PRRS endemic farm.
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spelling upm-614772022-04-05T02:56:39Z http://psasir.upm.edu.my/id/eprint/61477/ Different porcine reproductive and respiratory syndrome (PRRS) vaccine regimes and its effect on pig immunity status at Southeast Asia pig farms Cheah, Z. H. Ooi, Peck Toung Phang, Lai Yee Chua, V. Low, S. E. Cheah, H. C. Lim, E. Yong, C. K. Kam, K. Y. Porcine reproductive and respiratory syndrome (PRRS) is a disease characterised by late-term reproductive failure in sows and gilts, and respiratory problems in piglets and growing pigs. In this study, 240 sera were collected from four farms that had been practicing different PRRS vaccination regime for more than a year and vaccinations were done at 2 months before sampling. Fifteen sera samples from four age groups: sows, growers, weaners and piglets were collected from each farm and analysed using IDEXX PRRS X3 ELISA for PRRSV antibodies. Pooled serum samples were tested by using nested-PCR that enable the differentiation of Type I and Type II PRRSV. Out of 80 pooled serum samples, none were positive for PRRSV indicating all age groups were not viraemic after vaccination. Results by ELISA test showed all the farms were seropositive for PRRS. ELISA testing showed no significant difference between the farms except for Farm B which practised whole herd US MLV vaccination. Farm B showed significantly lower (p< 0.05) S/P ratio in their piglet, grower and sow groups which suggest there was low virus circulation in herd. Farm A which practised US MLV on sow was the only farm found to have seronegative status in their weaners. Data indicates PRRS MLV vaccination will not cause viraemia post four weeks vaccination and whole herd MLV vaccination may help to reduce virus circulation in PRRS endemic farm. Malaysian Society of Parasitology and Tropical Medicine 2017-06 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/61477/1/Different%20porcine%20reproductive%20and%20respiratory%20syndrome%20%28PRRS%29%20vaccine%20regimes%20and%20its%20effect%20on%20pig%20immunity%20status%20at%20Southeast%20Asia%20pig%20farms.pdf Cheah, Z. H. and Ooi, Peck Toung and Phang, Lai Yee and Chua, V. and Low, S. E. and Cheah, H. C. and Lim, E. and Yong, C. K. and Kam, K. Y. (2017) Different porcine reproductive and respiratory syndrome (PRRS) vaccine regimes and its effect on pig immunity status at Southeast Asia pig farms. Tropical Biomedicine, 34 (2). pp. 388-395. ISSN 0127-5720 https://pubmed.ncbi.nlm.nih.gov/33593019/
spellingShingle Cheah, Z. H.
Ooi, Peck Toung
Phang, Lai Yee
Chua, V.
Low, S. E.
Cheah, H. C.
Lim, E.
Yong, C. K.
Kam, K. Y.
Different porcine reproductive and respiratory syndrome (PRRS) vaccine regimes and its effect on pig immunity status at Southeast Asia pig farms
title Different porcine reproductive and respiratory syndrome (PRRS) vaccine regimes and its effect on pig immunity status at Southeast Asia pig farms
title_full Different porcine reproductive and respiratory syndrome (PRRS) vaccine regimes and its effect on pig immunity status at Southeast Asia pig farms
title_fullStr Different porcine reproductive and respiratory syndrome (PRRS) vaccine regimes and its effect on pig immunity status at Southeast Asia pig farms
title_full_unstemmed Different porcine reproductive and respiratory syndrome (PRRS) vaccine regimes and its effect on pig immunity status at Southeast Asia pig farms
title_short Different porcine reproductive and respiratory syndrome (PRRS) vaccine regimes and its effect on pig immunity status at Southeast Asia pig farms
title_sort different porcine reproductive and respiratory syndrome (prrs) vaccine regimes and its effect on pig immunity status at southeast asia pig farms
url http://psasir.upm.edu.my/id/eprint/61477/
http://psasir.upm.edu.my/id/eprint/61477/
http://psasir.upm.edu.my/id/eprint/61477/1/Different%20porcine%20reproductive%20and%20respiratory%20syndrome%20%28PRRS%29%20vaccine%20regimes%20and%20its%20effect%20on%20pig%20immunity%20status%20at%20Southeast%20Asia%20pig%20farms.pdf