Cryo-electron microscopy structure of the Macrobrachium rosenbergii nodavirus capsid at 7 angstroms resolution
White tail disease in the giant freshwater prawn Macrobrachium rosenbergii causes significant economic losses in shrimp farms and hatcheries and poses a threat to food-security in many developing countries. Outbreaks of Macrobrachium rosenbergii nodavirus (MrNV), the causative agent of white tail di...
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| Format: | Article |
| Language: | English |
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Nature Research
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/61261/ http://psasir.upm.edu.my/id/eprint/61261/1/TAIL.pdf |
| _version_ | 1848854376734523392 |
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| author | Ho, Kok Lian Kueh, Chare Li Beh, Poay Ling Tan, Wen Siang Bhella, David |
| author_facet | Ho, Kok Lian Kueh, Chare Li Beh, Poay Ling Tan, Wen Siang Bhella, David |
| author_sort | Ho, Kok Lian |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | White tail disease in the giant freshwater prawn Macrobrachium rosenbergii causes significant economic losses in shrimp farms and hatcheries and poses a threat to food-security in many developing countries. Outbreaks of Macrobrachium rosenbergii nodavirus (MrNV), the causative agent of white tail disease (WTD) are associated with up to 100% mortality rates. There are no interventions available to treat or prevent MrNV disease however. Here we show the structure of MrNV virus-like particles (VLPs) produced by recombinant expression of the capsid protein, using cryogenic electron microscopy. Our data show that MrNV VLPs package nucleic acids in a manner reminiscent of other known nodavirus structures. The structure of the capsid however shows striking differences from insect and fish infecting nodaviruses, which have been shown to assemble trimer-clustered T = 3 icosahedral virus particles. MrNV particles have pronounced dimeric blade-shaped spikes extending up to 6 nm from the outer surface of the capsid shell. Our structural analysis supports the assertion that MrNV may belong to a new genus of the Nodaviridae. Moreover, our study provides the first structural view of an important pathogen affecting aquaculture industries across the world. |
| first_indexed | 2025-11-15T11:08:53Z |
| format | Article |
| id | upm-61261 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:08:53Z |
| publishDate | 2017 |
| publisher | Nature Research |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-612612021-07-16T09:54:35Z http://psasir.upm.edu.my/id/eprint/61261/ Cryo-electron microscopy structure of the Macrobrachium rosenbergii nodavirus capsid at 7 angstroms resolution Ho, Kok Lian Kueh, Chare Li Beh, Poay Ling Tan, Wen Siang Bhella, David White tail disease in the giant freshwater prawn Macrobrachium rosenbergii causes significant economic losses in shrimp farms and hatcheries and poses a threat to food-security in many developing countries. Outbreaks of Macrobrachium rosenbergii nodavirus (MrNV), the causative agent of white tail disease (WTD) are associated with up to 100% mortality rates. There are no interventions available to treat or prevent MrNV disease however. Here we show the structure of MrNV virus-like particles (VLPs) produced by recombinant expression of the capsid protein, using cryogenic electron microscopy. Our data show that MrNV VLPs package nucleic acids in a manner reminiscent of other known nodavirus structures. The structure of the capsid however shows striking differences from insect and fish infecting nodaviruses, which have been shown to assemble trimer-clustered T = 3 icosahedral virus particles. MrNV particles have pronounced dimeric blade-shaped spikes extending up to 6 nm from the outer surface of the capsid shell. Our structural analysis supports the assertion that MrNV may belong to a new genus of the Nodaviridae. Moreover, our study provides the first structural view of an important pathogen affecting aquaculture industries across the world. Nature Research 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/61261/1/TAIL.pdf Ho, Kok Lian and Kueh, Chare Li and Beh, Poay Ling and Tan, Wen Siang and Bhella, David (2017) Cryo-electron microscopy structure of the Macrobrachium rosenbergii nodavirus capsid at 7 angstroms resolution. Scientific Reports, 7. art. no. 2083. pp. 1-8. ISSN 2045-2322 https://www.nature.com/articles/s41598-017-02292-0 10.1038/s41598-017-02292-0 |
| spellingShingle | Ho, Kok Lian Kueh, Chare Li Beh, Poay Ling Tan, Wen Siang Bhella, David Cryo-electron microscopy structure of the Macrobrachium rosenbergii nodavirus capsid at 7 angstroms resolution |
| title | Cryo-electron microscopy structure of the Macrobrachium rosenbergii nodavirus capsid at 7 angstroms resolution |
| title_full | Cryo-electron microscopy structure of the Macrobrachium rosenbergii nodavirus capsid at 7 angstroms resolution |
| title_fullStr | Cryo-electron microscopy structure of the Macrobrachium rosenbergii nodavirus capsid at 7 angstroms resolution |
| title_full_unstemmed | Cryo-electron microscopy structure of the Macrobrachium rosenbergii nodavirus capsid at 7 angstroms resolution |
| title_short | Cryo-electron microscopy structure of the Macrobrachium rosenbergii nodavirus capsid at 7 angstroms resolution |
| title_sort | cryo-electron microscopy structure of the macrobrachium rosenbergii nodavirus capsid at 7 angstroms resolution |
| url | http://psasir.upm.edu.my/id/eprint/61261/ http://psasir.upm.edu.my/id/eprint/61261/ http://psasir.upm.edu.my/id/eprint/61261/ http://psasir.upm.edu.my/id/eprint/61261/1/TAIL.pdf |