Chemopreventive effects of germinated rough rice crude extract in inhibiting azoxymethane-induced aberrant crypt foci formation in Sprague -Dawley rats
Chemoprevention has become an important area in cancer research due to low success rate of current therapeutic modalities. Diet plays a vital role in the etiology of cancer. This research was carried out to study the chemopreventive properties of germinated rough rice (GRR) crude extract in Sprague-...
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Hindawi
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/61109/ http://psasir.upm.edu.my/id/eprint/61109/1/Chemopreventive%20effects%20of%20germinated%20rough%20rice%20crude%20extract%20in%20inhibiting%20azoxymethane-induced%20aberrant%20crypt%20foci%20formation%20in%20Sprague-Dawley%20rats.pdf |
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| author | Saki, Elnaz Saiful Yazan, Latifah Mohd Ali, Razana Ahmad, Zalinah |
| author_facet | Saki, Elnaz Saiful Yazan, Latifah Mohd Ali, Razana Ahmad, Zalinah |
| author_sort | Saki, Elnaz |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Chemoprevention has become an important area in cancer research due to low success rate of current therapeutic modalities. Diet plays a vital role in the etiology of cancer. This research was carried out to study the chemopreventive properties of germinated rough rice (GRR) crude extract in Sprague-Dawley rats induced with azoxymethane. Germination of rough rice causes significant changes in several chemical compositions of presently bioactive compounds. These compounds may prevent or postpone the inception of cancer. Fifty male Sprague-Dawley rats (6 weeks of age) were randomly divided into 5 groups which were (G1) induced with azoxymethane (AOM) and not given GRR (positive control), (G2) induced with AOM and given 2000 mg/kg GRR, (G3) induced with AOM and given 1000 mg/kg GRR, (G4) induced with AOM and given 500 mg/kg GRR, and (G5) not induced with AOM and not given GRR crude extract (negative control). To induce colon cancer, rats received two IP injections of AOM in saline (15 mg/kg) for two subsequent weeks. Organs were removed and weighed. Aberrant crypt foci (ACF) were evaluated histopathologically. -Catenin expressions were determined by Western blot. Treatment with 2000 mg/kg GRR crude extract not only resulted in the greatest reduction in the size and number of ACF but also displayed the highest percentage of nondysplastic ACF. Treatment with 2000 mg/kg GRR also gave the lowest level of expression in β-catenin. Thus, GRR could be a promising dietary supplement for prevention of CRC. |
| first_indexed | 2025-11-15T11:08:20Z |
| format | Article |
| id | upm-61109 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:08:20Z |
| publishDate | 2017 |
| publisher | Hindawi |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-611092022-03-08T04:41:10Z http://psasir.upm.edu.my/id/eprint/61109/ Chemopreventive effects of germinated rough rice crude extract in inhibiting azoxymethane-induced aberrant crypt foci formation in Sprague -Dawley rats Saki, Elnaz Saiful Yazan, Latifah Mohd Ali, Razana Ahmad, Zalinah Chemoprevention has become an important area in cancer research due to low success rate of current therapeutic modalities. Diet plays a vital role in the etiology of cancer. This research was carried out to study the chemopreventive properties of germinated rough rice (GRR) crude extract in Sprague-Dawley rats induced with azoxymethane. Germination of rough rice causes significant changes in several chemical compositions of presently bioactive compounds. These compounds may prevent or postpone the inception of cancer. Fifty male Sprague-Dawley rats (6 weeks of age) were randomly divided into 5 groups which were (G1) induced with azoxymethane (AOM) and not given GRR (positive control), (G2) induced with AOM and given 2000 mg/kg GRR, (G3) induced with AOM and given 1000 mg/kg GRR, (G4) induced with AOM and given 500 mg/kg GRR, and (G5) not induced with AOM and not given GRR crude extract (negative control). To induce colon cancer, rats received two IP injections of AOM in saline (15 mg/kg) for two subsequent weeks. Organs were removed and weighed. Aberrant crypt foci (ACF) were evaluated histopathologically. -Catenin expressions were determined by Western blot. Treatment with 2000 mg/kg GRR crude extract not only resulted in the greatest reduction in the size and number of ACF but also displayed the highest percentage of nondysplastic ACF. Treatment with 2000 mg/kg GRR also gave the lowest level of expression in β-catenin. Thus, GRR could be a promising dietary supplement for prevention of CRC. Hindawi 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/61109/1/Chemopreventive%20effects%20of%20germinated%20rough%20rice%20crude%20extract%20in%20inhibiting%20azoxymethane-induced%20aberrant%20crypt%20foci%20formation%20in%20Sprague-Dawley%20rats.pdf Saki, Elnaz and Saiful Yazan, Latifah and Mohd Ali, Razana and Ahmad, Zalinah (2017) Chemopreventive effects of germinated rough rice crude extract in inhibiting azoxymethane-induced aberrant crypt foci formation in Sprague -Dawley rats. Biomed Research International, 2017. art. no. 9517287. pp. 1-9. ISSN 2314-6133; ESSN: 2314-6141 https://www.hindawi.com/journals/bmri/2017/9517287/ 10.1155/2017/9517287 |
| spellingShingle | Saki, Elnaz Saiful Yazan, Latifah Mohd Ali, Razana Ahmad, Zalinah Chemopreventive effects of germinated rough rice crude extract in inhibiting azoxymethane-induced aberrant crypt foci formation in Sprague -Dawley rats |
| title | Chemopreventive effects of germinated rough rice crude extract in inhibiting azoxymethane-induced aberrant crypt foci formation in Sprague -Dawley rats |
| title_full | Chemopreventive effects of germinated rough rice crude extract in inhibiting azoxymethane-induced aberrant crypt foci formation in Sprague -Dawley rats |
| title_fullStr | Chemopreventive effects of germinated rough rice crude extract in inhibiting azoxymethane-induced aberrant crypt foci formation in Sprague -Dawley rats |
| title_full_unstemmed | Chemopreventive effects of germinated rough rice crude extract in inhibiting azoxymethane-induced aberrant crypt foci formation in Sprague -Dawley rats |
| title_short | Chemopreventive effects of germinated rough rice crude extract in inhibiting azoxymethane-induced aberrant crypt foci formation in Sprague -Dawley rats |
| title_sort | chemopreventive effects of germinated rough rice crude extract in inhibiting azoxymethane-induced aberrant crypt foci formation in sprague -dawley rats |
| url | http://psasir.upm.edu.my/id/eprint/61109/ http://psasir.upm.edu.my/id/eprint/61109/ http://psasir.upm.edu.my/id/eprint/61109/ http://psasir.upm.edu.my/id/eprint/61109/1/Chemopreventive%20effects%20of%20germinated%20rough%20rice%20crude%20extract%20in%20inhibiting%20azoxymethane-induced%20aberrant%20crypt%20foci%20formation%20in%20Sprague-Dawley%20rats.pdf |