Characterization, drug release profile and cytotoxicity of dentatin-hydroxypropyl-β-cyclodextrin complex
This current work has been conducted mainly to increase solubility and drug release properties for high hydrophobic Dentatin (DEN) by incorporation it into Hydroxypropyl-β-Cyclodextrin (HPβCD) cavity. To confirm that inclusion be succeeded, the produced complex were installed onto different machines...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Springer Netherlands
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/61100/ http://psasir.upm.edu.my/id/eprint/61100/1/Characterization%2C%20drug%20release%20profile%20and%20cytotoxicity%20of%20dentatin-hydroxypropyl-%CE%B2-cyclodextrin%20complex.pdf |
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| author | Ashwaq, Al-Abboodi Shakir Abdullah, Rasedee @ Mat Abdul, Ahmad Bustamam Yap, Taufiq Yun Hin Al-Qubaisi, Mothanna Sadiq Eid, Eltayeb E. M. |
| author_facet | Ashwaq, Al-Abboodi Shakir Abdullah, Rasedee @ Mat Abdul, Ahmad Bustamam Yap, Taufiq Yun Hin Al-Qubaisi, Mothanna Sadiq Eid, Eltayeb E. M. |
| author_sort | Ashwaq, Al-Abboodi Shakir |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | This current work has been conducted mainly to increase solubility and drug release properties for high hydrophobic Dentatin (DEN) by incorporation it into Hydroxypropyl-β-Cyclodextrin (HPβCD) cavity. To confirm that inclusion be succeeded, the produced complex were installed onto different machines. The latter includes: Fourier transform infrared spectroscopy (FT-IR), X-ray diffractometry (XRD), differential scanning calorimetry (DSC), and field emission-scanning electron microscopy (FE-SEM). The hydrodynamic diameter and zeta potential of DEN-HPβCD complex were 2.025 ± 0.39 nm and −33.6 mV, respectively. Ultra-violet spectroscopy was employed to further confirmation of complexation process as well as to determine drug release profile. The result showed an initial burst release (19.9% within first two minutes) and then a continuous release for an extended period of 41 h (100%). The solubility of DEN was enhanced by >300 fold following complexation when a compared to DEN alone. Moreover, MTT finding showed that this complexation did not reduce cytotoxicity of DEN after applying on prostate cancer (LNCaP), human adenocarcinoma breast cancer (MDA-MB-231) and human gastric adenocarcinoma cell line (HDT). However, further investigations are required to validate efficacy of our produced inclusion using molecular analysis and in vivo studies. |
| first_indexed | 2025-11-15T11:08:18Z |
| format | Article |
| id | upm-61100 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:08:18Z |
| publishDate | 2017 |
| publisher | Springer Netherlands |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-611002018-09-20T04:04:21Z http://psasir.upm.edu.my/id/eprint/61100/ Characterization, drug release profile and cytotoxicity of dentatin-hydroxypropyl-β-cyclodextrin complex Ashwaq, Al-Abboodi Shakir Abdullah, Rasedee @ Mat Abdul, Ahmad Bustamam Yap, Taufiq Yun Hin Al-Qubaisi, Mothanna Sadiq Eid, Eltayeb E. M. This current work has been conducted mainly to increase solubility and drug release properties for high hydrophobic Dentatin (DEN) by incorporation it into Hydroxypropyl-β-Cyclodextrin (HPβCD) cavity. To confirm that inclusion be succeeded, the produced complex were installed onto different machines. The latter includes: Fourier transform infrared spectroscopy (FT-IR), X-ray diffractometry (XRD), differential scanning calorimetry (DSC), and field emission-scanning electron microscopy (FE-SEM). The hydrodynamic diameter and zeta potential of DEN-HPβCD complex were 2.025 ± 0.39 nm and −33.6 mV, respectively. Ultra-violet spectroscopy was employed to further confirmation of complexation process as well as to determine drug release profile. The result showed an initial burst release (19.9% within first two minutes) and then a continuous release for an extended period of 41 h (100%). The solubility of DEN was enhanced by >300 fold following complexation when a compared to DEN alone. Moreover, MTT finding showed that this complexation did not reduce cytotoxicity of DEN after applying on prostate cancer (LNCaP), human adenocarcinoma breast cancer (MDA-MB-231) and human gastric adenocarcinoma cell line (HDT). However, further investigations are required to validate efficacy of our produced inclusion using molecular analysis and in vivo studies. Springer Netherlands 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/61100/1/Characterization%2C%20drug%20release%20profile%20and%20cytotoxicity%20of%20dentatin-hydroxypropyl-%CE%B2-cyclodextrin%20complex.pdf Ashwaq, Al-Abboodi Shakir and Abdullah, Rasedee @ Mat and Abdul, Ahmad Bustamam and Yap, Taufiq Yun Hin and Al-Qubaisi, Mothanna Sadiq and Eid, Eltayeb E. M. (2017) Characterization, drug release profile and cytotoxicity of dentatin-hydroxypropyl-β-cyclodextrin complex. Journal of Inclusion Phenomena and Macrocyclic Chemistry, 87. 167 - 178. ISSN 1388-3127 https://link.springer.com/content/pdf/10.1007%2Fs10847-016-0688-y.pdf 10.1007/s10847-016-0688-y |
| spellingShingle | Ashwaq, Al-Abboodi Shakir Abdullah, Rasedee @ Mat Abdul, Ahmad Bustamam Yap, Taufiq Yun Hin Al-Qubaisi, Mothanna Sadiq Eid, Eltayeb E. M. Characterization, drug release profile and cytotoxicity of dentatin-hydroxypropyl-β-cyclodextrin complex |
| title | Characterization, drug release profile and cytotoxicity of dentatin-hydroxypropyl-β-cyclodextrin complex |
| title_full | Characterization, drug release profile and cytotoxicity of dentatin-hydroxypropyl-β-cyclodextrin complex |
| title_fullStr | Characterization, drug release profile and cytotoxicity of dentatin-hydroxypropyl-β-cyclodextrin complex |
| title_full_unstemmed | Characterization, drug release profile and cytotoxicity of dentatin-hydroxypropyl-β-cyclodextrin complex |
| title_short | Characterization, drug release profile and cytotoxicity of dentatin-hydroxypropyl-β-cyclodextrin complex |
| title_sort | characterization, drug release profile and cytotoxicity of dentatin-hydroxypropyl-β-cyclodextrin complex |
| url | http://psasir.upm.edu.my/id/eprint/61100/ http://psasir.upm.edu.my/id/eprint/61100/ http://psasir.upm.edu.my/id/eprint/61100/ http://psasir.upm.edu.my/id/eprint/61100/1/Characterization%2C%20drug%20release%20profile%20and%20cytotoxicity%20of%20dentatin-hydroxypropyl-%CE%B2-cyclodextrin%20complex.pdf |