Characterization of Lasiodiplodia theobromae and L. pseudotheobromae causing fruit rot on pre-harvest mango in Malaysia
Lasiodiplodia species are important plant pathogens in mango production countries, infecting plants during both pre- and post- harvest phases. Fruit rot reduces mango production and creates tremendous losses in economy of the involved countries. Fungal isolation was conducted in eight locations...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Mushroom Research Foundation
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/61096/ http://psasir.upm.edu.my/id/eprint/61096/1/Characterization%20of%20Lasiodiplodia%20theobromae%20and%20L.%20pseudotheobromae%20causing%20fruit%20rot%20on%20pre-harvest%20mango%20in%20Malaysia.pdf |
| _version_ | 1848854338411167744 |
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| author | M. S., Munirah A. R., Azmi Yong, Christina Seok Yien Mohd Zainudin, Nur Ain Izzati |
| author_facet | M. S., Munirah A. R., Azmi Yong, Christina Seok Yien Mohd Zainudin, Nur Ain Izzati |
| author_sort | M. S., Munirah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Lasiodiplodia species are important plant pathogens in mango production countries, infecting plants during both pre- and post- harvest phases. Fruit rot reduces mango production and creates tremendous losses in economy of the involved countries. Fungal isolation was conducted in eight locations throughout Peninsular Malaysia. Species identification was based on morphological characterisation and internal transcribed spacer (ITS) and β-tubulin (BT2) gene sequences analysis. Twenty isolates were obtained and identified as
Lasiodiplodia theobromae (17isolates) and L. pseudotheobromae (3 isolates). Phylogenetic analysis using maximum-likelihood method demonstrated that all isolates of L. theobromae and L. pseudotheobromae formed a monophyletic clade. In pathogenicity tests, most fungal isolates inoculated on mango fruit showed fruit rot
symptom. The most virulent isolate was L. theobromae
A1718 with disease severity index (DSI)of 87.5%. |
| first_indexed | 2025-11-15T11:08:17Z |
| format | Article |
| id | upm-61096 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:08:17Z |
| publishDate | 2017 |
| publisher | Mushroom Research Foundation |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-610962018-09-20T03:21:40Z http://psasir.upm.edu.my/id/eprint/61096/ Characterization of Lasiodiplodia theobromae and L. pseudotheobromae causing fruit rot on pre-harvest mango in Malaysia M. S., Munirah A. R., Azmi Yong, Christina Seok Yien Mohd Zainudin, Nur Ain Izzati Lasiodiplodia species are important plant pathogens in mango production countries, infecting plants during both pre- and post- harvest phases. Fruit rot reduces mango production and creates tremendous losses in economy of the involved countries. Fungal isolation was conducted in eight locations throughout Peninsular Malaysia. Species identification was based on morphological characterisation and internal transcribed spacer (ITS) and β-tubulin (BT2) gene sequences analysis. Twenty isolates were obtained and identified as Lasiodiplodia theobromae (17isolates) and L. pseudotheobromae (3 isolates). Phylogenetic analysis using maximum-likelihood method demonstrated that all isolates of L. theobromae and L. pseudotheobromae formed a monophyletic clade. In pathogenicity tests, most fungal isolates inoculated on mango fruit showed fruit rot symptom. The most virulent isolate was L. theobromae A1718 with disease severity index (DSI)of 87.5%. Mushroom Research Foundation 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/61096/1/Characterization%20of%20Lasiodiplodia%20theobromae%20and%20L.%20pseudotheobromae%20causing%20fruit%20rot%20on%20pre-harvest%20mango%20in%20Malaysia.pdf M. S., Munirah and A. R., Azmi and Yong, Christina Seok Yien and Mohd Zainudin, Nur Ain Izzati (2017) Characterization of Lasiodiplodia theobromae and L. pseudotheobromae causing fruit rot on pre-harvest mango in Malaysia. Plant Pathology & Quarantine, 7 (2). pp. 202-213. ISSN 2229-2217 http://www.plantpathologyquarantine.org/pdf/PPQ_7_2_14.pdf 10.5943/ppq/7/2/14 |
| spellingShingle | M. S., Munirah A. R., Azmi Yong, Christina Seok Yien Mohd Zainudin, Nur Ain Izzati Characterization of Lasiodiplodia theobromae and L. pseudotheobromae causing fruit rot on pre-harvest mango in Malaysia |
| title | Characterization of Lasiodiplodia theobromae and L. pseudotheobromae causing fruit rot on pre-harvest mango in Malaysia |
| title_full | Characterization of Lasiodiplodia theobromae and L. pseudotheobromae causing fruit rot on pre-harvest mango in Malaysia |
| title_fullStr | Characterization of Lasiodiplodia theobromae and L. pseudotheobromae causing fruit rot on pre-harvest mango in Malaysia |
| title_full_unstemmed | Characterization of Lasiodiplodia theobromae and L. pseudotheobromae causing fruit rot on pre-harvest mango in Malaysia |
| title_short | Characterization of Lasiodiplodia theobromae and L. pseudotheobromae causing fruit rot on pre-harvest mango in Malaysia |
| title_sort | characterization of lasiodiplodia theobromae and l. pseudotheobromae causing fruit rot on pre-harvest mango in malaysia |
| url | http://psasir.upm.edu.my/id/eprint/61096/ http://psasir.upm.edu.my/id/eprint/61096/ http://psasir.upm.edu.my/id/eprint/61096/ http://psasir.upm.edu.my/id/eprint/61096/1/Characterization%20of%20Lasiodiplodia%20theobromae%20and%20L.%20pseudotheobromae%20causing%20fruit%20rot%20on%20pre-harvest%20mango%20in%20Malaysia.pdf |