Characterization of antioxidant activity of Momordica charantia fruit by infrared-based fingerprinting
Momordica charantia is widely consumed edible fruit. The food and pharmaceutical industries use it as a natural antioxidant. However, the quality control of M. charantia-based medicinal products is questionable due to the complexity of metabolites in this fruit. Hence, this study has developed a sta...
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| Format: | Article |
| Language: | English |
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Taylor & Francis
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/61091/ http://psasir.upm.edu.my/id/eprint/61091/1/Characterization%20of%20antioxidant%20activity%20of%20Momordica%20charantia%20fruit%20by%20infrared-based%20fingerprinting.pdf |
| _version_ | 1848854336985104384 |
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| author | Khatib, Alfi Perumal, Vikneswari Ahmed, Qamar Uddin Uzir, Bisha Fathamah Abas, Faridah Murugesu, Suganya |
| author_facet | Khatib, Alfi Perumal, Vikneswari Ahmed, Qamar Uddin Uzir, Bisha Fathamah Abas, Faridah Murugesu, Suganya |
| author_sort | Khatib, Alfi |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Momordica charantia is widely consumed edible fruit. The food and pharmaceutical industries use it as a natural antioxidant. However, the quality control of M. charantia-based medicinal products is questionable due to the complexity of metabolites in this fruit. Hence, this study has developed a statistical model in predicting the antioxidant value through the 2, 2-diphenyl-1 picrylhydrazyl radical scavenging activity and ferric reducing antioxidant power based on infrared spectroscopy with attenuated total reflectance. This technique was reliably used for quality control. Six ethanol extracts (0, 20, 40, 60, 80, and 100% in water) of this plant’s fruit were prepared. The radical scavenging and ferric reducing antioxidant power activities were measured and the chemical profiling of the extracts was fingerprinted by infrared spectroscopy between 4,000 and 600 cm−1 at a resolution of 4 cm−1. Statistical analysis was developed by correlating the bioactivity and infrared spectra of each extract using orthogonal partial least square discriminant analysis. The C–N, C˭O, C–O, C–H, and OH infrared signals were positively correlated with biological activity. The antioxidant activity of the fruit of M. charantia may be due to the presence of several antioxidants that work synergistically. |
| first_indexed | 2025-11-15T11:08:15Z |
| format | Article |
| id | upm-61091 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:08:15Z |
| publishDate | 2017 |
| publisher | Taylor & Francis |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-610912019-05-06T05:17:26Z http://psasir.upm.edu.my/id/eprint/61091/ Characterization of antioxidant activity of Momordica charantia fruit by infrared-based fingerprinting Khatib, Alfi Perumal, Vikneswari Ahmed, Qamar Uddin Uzir, Bisha Fathamah Abas, Faridah Murugesu, Suganya Momordica charantia is widely consumed edible fruit. The food and pharmaceutical industries use it as a natural antioxidant. However, the quality control of M. charantia-based medicinal products is questionable due to the complexity of metabolites in this fruit. Hence, this study has developed a statistical model in predicting the antioxidant value through the 2, 2-diphenyl-1 picrylhydrazyl radical scavenging activity and ferric reducing antioxidant power based on infrared spectroscopy with attenuated total reflectance. This technique was reliably used for quality control. Six ethanol extracts (0, 20, 40, 60, 80, and 100% in water) of this plant’s fruit were prepared. The radical scavenging and ferric reducing antioxidant power activities were measured and the chemical profiling of the extracts was fingerprinted by infrared spectroscopy between 4,000 and 600 cm−1 at a resolution of 4 cm−1. Statistical analysis was developed by correlating the bioactivity and infrared spectra of each extract using orthogonal partial least square discriminant analysis. The C–N, C˭O, C–O, C–H, and OH infrared signals were positively correlated with biological activity. The antioxidant activity of the fruit of M. charantia may be due to the presence of several antioxidants that work synergistically. Taylor & Francis 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/61091/1/Characterization%20of%20antioxidant%20activity%20of%20Momordica%20charantia%20fruit%20by%20infrared-based%20fingerprinting.pdf Khatib, Alfi and Perumal, Vikneswari and Ahmed, Qamar Uddin and Uzir, Bisha Fathamah and Abas, Faridah and Murugesu, Suganya (2017) Characterization of antioxidant activity of Momordica charantia fruit by infrared-based fingerprinting. Analytical Letters, 50 (12). 1977 - 1991. ISSN 0003-2719; ESSN: 1532-236X https://www.tandfonline.com/doi/abs/10.1080/00032719.2016.1261877 10.1080/00032719.2016.1261877 |
| spellingShingle | Khatib, Alfi Perumal, Vikneswari Ahmed, Qamar Uddin Uzir, Bisha Fathamah Abas, Faridah Murugesu, Suganya Characterization of antioxidant activity of Momordica charantia fruit by infrared-based fingerprinting |
| title | Characterization of antioxidant activity of Momordica charantia fruit by infrared-based fingerprinting |
| title_full | Characterization of antioxidant activity of Momordica charantia fruit by infrared-based fingerprinting |
| title_fullStr | Characterization of antioxidant activity of Momordica charantia fruit by infrared-based fingerprinting |
| title_full_unstemmed | Characterization of antioxidant activity of Momordica charantia fruit by infrared-based fingerprinting |
| title_short | Characterization of antioxidant activity of Momordica charantia fruit by infrared-based fingerprinting |
| title_sort | characterization of antioxidant activity of momordica charantia fruit by infrared-based fingerprinting |
| url | http://psasir.upm.edu.my/id/eprint/61091/ http://psasir.upm.edu.my/id/eprint/61091/ http://psasir.upm.edu.my/id/eprint/61091/ http://psasir.upm.edu.my/id/eprint/61091/1/Characterization%20of%20antioxidant%20activity%20of%20Momordica%20charantia%20fruit%20by%20infrared-based%20fingerprinting.pdf |