Blockade of eosinophil-induced bronchial epithelial-mesenchymal transition with a geranyl acetophenone in a coculture model
Epithelial-mesenchymal transition (EMT) is currently recognized as the main cellular event that contributes to airway remodeling. Eosinophils can induce EMT in airway epithelial cells via increased transforming growth factor (TGF)-β production. We assessed the effect of synthetic 2,4,6-trihydroxy-3-...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Research Foundation
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/61018/ http://psasir.upm.edu.my/id/eprint/61018/1/Blockade%20of%20eosinophil-induced%20bronchial%20epithelial-mesenchymal%20transition%20with%20a%20geranyl%20acetophenone%20in%20a%20coculture%20model.pdf |
| _version_ | 1848854320628367360 |
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| author | Lee, Yu Zhao Yap, Hui Min Shaari, Khozirah Tham, Chau Ling Sulaiman, Mohd Roslan Israf Ali, Daud Ahmad |
| author_facet | Lee, Yu Zhao Yap, Hui Min Shaari, Khozirah Tham, Chau Ling Sulaiman, Mohd Roslan Israf Ali, Daud Ahmad |
| author_sort | Lee, Yu Zhao |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Epithelial-mesenchymal transition (EMT) is currently recognized as the main cellular event that contributes to airway remodeling. Eosinophils can induce EMT in airway epithelial cells via increased transforming growth factor (TGF)-β production. We assessed the effect of synthetic 2,4,6-trihydroxy-3-geranyl acetophenone (tHGA) upon eosinophil-induced EMT in a cellular model. The human eosinophil cell line EoL-1 was used to induce EMT in BEAS-2B human bronchial epithelial cells. The induction of EMT was dose-dependently suppressed following tHGA treatment in which the epithelial morphology and E-cadherin expression were not altered. Protein and mRNA expression of vimentin, collagen I and fibronectin in eosinophil-induced epithelial cells were also significantly suppressed by tHGA treatment. Following pathway analysis, we showed that tHGA suppressed eosinophil-induced activator protein-1-mediated TGF-β production by targeting c-Jun N-terminal kinase and phosphoinositide 3-kinase signaling pathways. These findings corroborated previous findings on the ability of tHGA to inhibit experimental murine airway remodeling. |
| first_indexed | 2025-11-15T11:08:00Z |
| format | Article |
| id | upm-61018 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:08:00Z |
| publishDate | 2017 |
| publisher | Frontiers Research Foundation |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-610182019-04-30T03:45:34Z http://psasir.upm.edu.my/id/eprint/61018/ Blockade of eosinophil-induced bronchial epithelial-mesenchymal transition with a geranyl acetophenone in a coculture model Lee, Yu Zhao Yap, Hui Min Shaari, Khozirah Tham, Chau Ling Sulaiman, Mohd Roslan Israf Ali, Daud Ahmad Epithelial-mesenchymal transition (EMT) is currently recognized as the main cellular event that contributes to airway remodeling. Eosinophils can induce EMT in airway epithelial cells via increased transforming growth factor (TGF)-β production. We assessed the effect of synthetic 2,4,6-trihydroxy-3-geranyl acetophenone (tHGA) upon eosinophil-induced EMT in a cellular model. The human eosinophil cell line EoL-1 was used to induce EMT in BEAS-2B human bronchial epithelial cells. The induction of EMT was dose-dependently suppressed following tHGA treatment in which the epithelial morphology and E-cadherin expression were not altered. Protein and mRNA expression of vimentin, collagen I and fibronectin in eosinophil-induced epithelial cells were also significantly suppressed by tHGA treatment. Following pathway analysis, we showed that tHGA suppressed eosinophil-induced activator protein-1-mediated TGF-β production by targeting c-Jun N-terminal kinase and phosphoinositide 3-kinase signaling pathways. These findings corroborated previous findings on the ability of tHGA to inhibit experimental murine airway remodeling. Frontiers Research Foundation 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/61018/1/Blockade%20of%20eosinophil-induced%20bronchial%20epithelial-mesenchymal%20transition%20with%20a%20geranyl%20acetophenone%20in%20a%20coculture%20model.pdf Lee, Yu Zhao and Yap, Hui Min and Shaari, Khozirah and Tham, Chau Ling and Sulaiman, Mohd Roslan and Israf Ali, Daud Ahmad (2017) Blockade of eosinophil-induced bronchial epithelial-mesenchymal transition with a geranyl acetophenone in a coculture model. Frontiers in Pharmacology, 8. pp. 1-13. ISSN ESSN: 1663-9812 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5696322/pdf/fphar-08-00837.pdf 10.3389/fphar.2017.00837 |
| spellingShingle | Lee, Yu Zhao Yap, Hui Min Shaari, Khozirah Tham, Chau Ling Sulaiman, Mohd Roslan Israf Ali, Daud Ahmad Blockade of eosinophil-induced bronchial epithelial-mesenchymal transition with a geranyl acetophenone in a coculture model |
| title | Blockade of eosinophil-induced bronchial epithelial-mesenchymal transition with a geranyl acetophenone in a coculture model |
| title_full | Blockade of eosinophil-induced bronchial epithelial-mesenchymal transition with a geranyl acetophenone in a coculture model |
| title_fullStr | Blockade of eosinophil-induced bronchial epithelial-mesenchymal transition with a geranyl acetophenone in a coculture model |
| title_full_unstemmed | Blockade of eosinophil-induced bronchial epithelial-mesenchymal transition with a geranyl acetophenone in a coculture model |
| title_short | Blockade of eosinophil-induced bronchial epithelial-mesenchymal transition with a geranyl acetophenone in a coculture model |
| title_sort | blockade of eosinophil-induced bronchial epithelial-mesenchymal transition with a geranyl acetophenone in a coculture model |
| url | http://psasir.upm.edu.my/id/eprint/61018/ http://psasir.upm.edu.my/id/eprint/61018/ http://psasir.upm.edu.my/id/eprint/61018/ http://psasir.upm.edu.my/id/eprint/61018/1/Blockade%20of%20eosinophil-induced%20bronchial%20epithelial-mesenchymal%20transition%20with%20a%20geranyl%20acetophenone%20in%20a%20coculture%20model.pdf |