Artonin E induces p53-independent g1 cell cycle arrest and apoptosis through ROS-mediated mitochondrial pathway and livin suppression in MCF-7 cell
Artonin E is a prenylated flavonoid compound isolated from the stem bark of Artocarpus elasticus. This phytochemical has been previously reported to be drug-like with full compliance to Lipinski's rule of five and good physicochemical properties when compared with 95% of orally available drugs....
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/60929/ http://psasir.upm.edu.my/id/eprint/60929/1/Artonin%20E%20induces%20p53-independent%20g1%20cell%20cycle%20arrest%20and%20apoptosis%20through%20ROS-mediated%20mitochondrial%20pathway%20and%20livin%20suppression%20in%20MCF-7%20cell.pdf |
| _version_ | 1848854300921430016 |
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| author | Etti, Imaobong Christopher Abdullah, Rasedee @ Mat Mohd Hashim, Najihah Abdul, Ahmad Bustamam Kadir, Arifah Swee, Keong Yeap Waziri, Peter Malami, Ibrahim Kian, Lam Lim Etti, Christopher J. |
| author_facet | Etti, Imaobong Christopher Abdullah, Rasedee @ Mat Mohd Hashim, Najihah Abdul, Ahmad Bustamam Kadir, Arifah Swee, Keong Yeap Waziri, Peter Malami, Ibrahim Kian, Lam Lim Etti, Christopher J. |
| author_sort | Etti, Imaobong Christopher |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Artonin E is a prenylated flavonoid compound isolated from the stem bark of Artocarpus elasticus. This phytochemical has been previously reported to be drug-like with full compliance to Lipinski's rule of five and good physicochemical properties when compared with 95% of orally available drugs. It has also been shown to possess unique medicinal properties that can be utilized in view of alleviating most human disease conditions. In this study, we investigated the cytotoxic mechanism of Artonin E in MCF-7 breast cancer cells, which has so far not been reported. In this context, Artonin E significantly suppressed the breast cancer cell's viability while inducing apoptosis in a dose-dependent manner. This apoptosis induction was caspase dependent, and it is mediated mainly through the intrinsic pathway with the elevation of total reactive oxygen species. Gene and protein expression studies revealed significant upregulation of cytochrome c, Bax, caspases 7 and 9, and p21 in Artonin E-treated MCF-7 cells, while MAPK and cyclin D were downregulated. Livin, a member of the inhibitors of apoptosis, whose upregulation has been noted to precede chemotherapeutic resistance and apoptosis evasion was remarkably repressed. In all, Artonin E stood high as a potential agent in the treatment of breast cancer. |
| first_indexed | 2025-11-15T11:07:41Z |
| format | Article |
| id | upm-60929 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:07:41Z |
| publishDate | 2017 |
| publisher | Dove Medical Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-609292019-03-22T00:47:19Z http://psasir.upm.edu.my/id/eprint/60929/ Artonin E induces p53-independent g1 cell cycle arrest and apoptosis through ROS-mediated mitochondrial pathway and livin suppression in MCF-7 cell Etti, Imaobong Christopher Abdullah, Rasedee @ Mat Mohd Hashim, Najihah Abdul, Ahmad Bustamam Kadir, Arifah Swee, Keong Yeap Waziri, Peter Malami, Ibrahim Kian, Lam Lim Etti, Christopher J. Artonin E is a prenylated flavonoid compound isolated from the stem bark of Artocarpus elasticus. This phytochemical has been previously reported to be drug-like with full compliance to Lipinski's rule of five and good physicochemical properties when compared with 95% of orally available drugs. It has also been shown to possess unique medicinal properties that can be utilized in view of alleviating most human disease conditions. In this study, we investigated the cytotoxic mechanism of Artonin E in MCF-7 breast cancer cells, which has so far not been reported. In this context, Artonin E significantly suppressed the breast cancer cell's viability while inducing apoptosis in a dose-dependent manner. This apoptosis induction was caspase dependent, and it is mediated mainly through the intrinsic pathway with the elevation of total reactive oxygen species. Gene and protein expression studies revealed significant upregulation of cytochrome c, Bax, caspases 7 and 9, and p21 in Artonin E-treated MCF-7 cells, while MAPK and cyclin D were downregulated. Livin, a member of the inhibitors of apoptosis, whose upregulation has been noted to precede chemotherapeutic resistance and apoptosis evasion was remarkably repressed. In all, Artonin E stood high as a potential agent in the treatment of breast cancer. Dove Medical Press 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/60929/1/Artonin%20E%20induces%20p53-independent%20g1%20cell%20cycle%20arrest%20and%20apoptosis%20through%20ROS-mediated%20mitochondrial%20pathway%20and%20livin%20suppression%20in%20MCF-7%20cell.pdf Etti, Imaobong Christopher and Abdullah, Rasedee @ Mat and Mohd Hashim, Najihah and Abdul, Ahmad Bustamam and Kadir, Arifah and Swee, Keong Yeap and Waziri, Peter and Malami, Ibrahim and Kian, Lam Lim and Etti, Christopher J. (2017) Artonin E induces p53-independent g1 cell cycle arrest and apoptosis through ROS-mediated mitochondrial pathway and livin suppression in MCF-7 cell. Drug Design Development and Therapy, 11. 865 - 879. ISSN 1177-8881 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5367776/pdf/dddt-11-865.pdf 10.2147/DDDT.S124324 |
| spellingShingle | Etti, Imaobong Christopher Abdullah, Rasedee @ Mat Mohd Hashim, Najihah Abdul, Ahmad Bustamam Kadir, Arifah Swee, Keong Yeap Waziri, Peter Malami, Ibrahim Kian, Lam Lim Etti, Christopher J. Artonin E induces p53-independent g1 cell cycle arrest and apoptosis through ROS-mediated mitochondrial pathway and livin suppression in MCF-7 cell |
| title | Artonin E induces p53-independent g1 cell cycle arrest and apoptosis through ROS-mediated mitochondrial pathway and livin suppression in MCF-7 cell |
| title_full | Artonin E induces p53-independent g1 cell cycle arrest and apoptosis through ROS-mediated mitochondrial pathway and livin suppression in MCF-7 cell |
| title_fullStr | Artonin E induces p53-independent g1 cell cycle arrest and apoptosis through ROS-mediated mitochondrial pathway and livin suppression in MCF-7 cell |
| title_full_unstemmed | Artonin E induces p53-independent g1 cell cycle arrest and apoptosis through ROS-mediated mitochondrial pathway and livin suppression in MCF-7 cell |
| title_short | Artonin E induces p53-independent g1 cell cycle arrest and apoptosis through ROS-mediated mitochondrial pathway and livin suppression in MCF-7 cell |
| title_sort | artonin e induces p53-independent g1 cell cycle arrest and apoptosis through ros-mediated mitochondrial pathway and livin suppression in mcf-7 cell |
| url | http://psasir.upm.edu.my/id/eprint/60929/ http://psasir.upm.edu.my/id/eprint/60929/ http://psasir.upm.edu.my/id/eprint/60929/ http://psasir.upm.edu.my/id/eprint/60929/1/Artonin%20E%20induces%20p53-independent%20g1%20cell%20cycle%20arrest%20and%20apoptosis%20through%20ROS-mediated%20mitochondrial%20pathway%20and%20livin%20suppression%20in%20MCF-7%20cell.pdf |