Antibacterial activity of ethanolic extract of Syzygium polyanthum L. (Salam) leaves against foodborne pathogens and application as food sanitizer

The aim of this study was to determine antibacterial activity of S. polyanthum L. (salam) leaves extract foodborne pathogens. All the foodborne pathogens were inhibited after treating with extract in disk diffusion test with range 6.67 ± 0.58-9.67 ± 0.58 mm of inhibition zone. The range of MIC value...

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Main Authors: Ramli, Suzita, Radu, Son, Shaari, Khozirah, Rukayadi, Yaya
Format: Article
Language:English
Published: Hindawi Publishing Corporation 2017
Online Access:http://psasir.upm.edu.my/id/eprint/60879/
http://psasir.upm.edu.my/id/eprint/60879/1/Antibacterial%20activity%20of%20ethanolic%20extract%20of%20Syzygium%20polyanthum%20L.%20%28Salam%29%20leaves%20against%20foodborne%20pathogens%20and%20application%20as%20food%20sanitizer.pdf
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author Ramli, Suzita
Radu, Son
Shaari, Khozirah
Rukayadi, Yaya
author_facet Ramli, Suzita
Radu, Son
Shaari, Khozirah
Rukayadi, Yaya
author_sort Ramli, Suzita
building UPM Institutional Repository
collection Online Access
description The aim of this study was to determine antibacterial activity of S. polyanthum L. (salam) leaves extract foodborne pathogens. All the foodborne pathogens were inhibited after treating with extract in disk diffusion test with range 6.67 ± 0.58-9.67 ± 0.58 mm of inhibition zone. The range of MIC values was between 0.63 and 1.25 mg/mL whereas MBC values were in the range 0.63 mg/mL to 2.50 mg/mL. In time-kill curve, L. monocytogenes and P. aeruginosa were found completely killed after exposing to extract in 1 h incubation at 4x MIC. Four hours had been taken to completely kill E. coli, S. aureus, V. cholerae, and V. parahaemolyticus at 4x MIC. However, the population of K. pneumoniae, P. mirabilis, and S. typhimurium only reduced to 3 log CFU/mL. The treated cell showed cell rupture and leakage of the cell cytoplasm in SEM observation. The significant reduction of natural microflora in grapes fruit was started at 0.50% of extract at 5 min and this concentration also was parallel to sensory attributes acceptability where application of extract was accepted by the panellists until 5%. In conclusion, S. polyanthum extract exhibits antimicrobial activities and thus might be developed as natural sanitizer for washing raw food materials.
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spelling upm-608792019-03-26T08:46:18Z http://psasir.upm.edu.my/id/eprint/60879/ Antibacterial activity of ethanolic extract of Syzygium polyanthum L. (Salam) leaves against foodborne pathogens and application as food sanitizer Ramli, Suzita Radu, Son Shaari, Khozirah Rukayadi, Yaya The aim of this study was to determine antibacterial activity of S. polyanthum L. (salam) leaves extract foodborne pathogens. All the foodborne pathogens were inhibited after treating with extract in disk diffusion test with range 6.67 ± 0.58-9.67 ± 0.58 mm of inhibition zone. The range of MIC values was between 0.63 and 1.25 mg/mL whereas MBC values were in the range 0.63 mg/mL to 2.50 mg/mL. In time-kill curve, L. monocytogenes and P. aeruginosa were found completely killed after exposing to extract in 1 h incubation at 4x MIC. Four hours had been taken to completely kill E. coli, S. aureus, V. cholerae, and V. parahaemolyticus at 4x MIC. However, the population of K. pneumoniae, P. mirabilis, and S. typhimurium only reduced to 3 log CFU/mL. The treated cell showed cell rupture and leakage of the cell cytoplasm in SEM observation. The significant reduction of natural microflora in grapes fruit was started at 0.50% of extract at 5 min and this concentration also was parallel to sensory attributes acceptability where application of extract was accepted by the panellists until 5%. In conclusion, S. polyanthum extract exhibits antimicrobial activities and thus might be developed as natural sanitizer for washing raw food materials. Hindawi Publishing Corporation 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/60879/1/Antibacterial%20activity%20of%20ethanolic%20extract%20of%20Syzygium%20polyanthum%20L.%20%28Salam%29%20leaves%20against%20foodborne%20pathogens%20and%20application%20as%20food%20sanitizer.pdf Ramli, Suzita and Radu, Son and Shaari, Khozirah and Rukayadi, Yaya (2017) Antibacterial activity of ethanolic extract of Syzygium polyanthum L. (Salam) leaves against foodborne pathogens and application as food sanitizer. BioMed Research International. art. no. 9024246. pp. 1-13. ISSN 2314-6133; ESSN: 2314-6141 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5749218/pdf/BMRI2017-9024246.pdf 10.1155/2017/9024246
spellingShingle Ramli, Suzita
Radu, Son
Shaari, Khozirah
Rukayadi, Yaya
Antibacterial activity of ethanolic extract of Syzygium polyanthum L. (Salam) leaves against foodborne pathogens and application as food sanitizer
title Antibacterial activity of ethanolic extract of Syzygium polyanthum L. (Salam) leaves against foodborne pathogens and application as food sanitizer
title_full Antibacterial activity of ethanolic extract of Syzygium polyanthum L. (Salam) leaves against foodborne pathogens and application as food sanitizer
title_fullStr Antibacterial activity of ethanolic extract of Syzygium polyanthum L. (Salam) leaves against foodborne pathogens and application as food sanitizer
title_full_unstemmed Antibacterial activity of ethanolic extract of Syzygium polyanthum L. (Salam) leaves against foodborne pathogens and application as food sanitizer
title_short Antibacterial activity of ethanolic extract of Syzygium polyanthum L. (Salam) leaves against foodborne pathogens and application as food sanitizer
title_sort antibacterial activity of ethanolic extract of syzygium polyanthum l. (salam) leaves against foodborne pathogens and application as food sanitizer
url http://psasir.upm.edu.my/id/eprint/60879/
http://psasir.upm.edu.my/id/eprint/60879/
http://psasir.upm.edu.my/id/eprint/60879/
http://psasir.upm.edu.my/id/eprint/60879/1/Antibacterial%20activity%20of%20ethanolic%20extract%20of%20Syzygium%20polyanthum%20L.%20%28Salam%29%20leaves%20against%20foodborne%20pathogens%20and%20application%20as%20food%20sanitizer.pdf