Annona muricata leaves extracts prevent DMBA/TPA-induced skin tumorigenesis via modulating antioxidants enzyme system in ICR mice

Annona muricata, locally known as soursop has been reported to exhibit antiproliferative activities against various cancer cell lines. In this current study, we have investigated the antitumor promotion of various fractions of Annona muricata leaves (AML); hexane (AMLH), dichloromethane (AMLD) and m...

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Main Authors: Md Roduan, Mohd Rohaizad, Abd Hamid @ Abdul Razak, Roslida, Sulaiman, Hamizah, Mohtarrudin, Norhafizah
Format: Article
Language:English
Published: Elsevier 2017
Online Access:http://psasir.upm.edu.my/id/eprint/60868/
http://psasir.upm.edu.my/id/eprint/60868/1/Annona%20muricata%20leaves%20extracts%20prevent%20DMBATPA-induced%20skin%20tumorigenesis%20via%20modulating%20antioxidants%20enzyme%20system%20in%20ICR%20mice.pdf
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author Md Roduan, Mohd Rohaizad
Abd Hamid @ Abdul Razak, Roslida
Sulaiman, Hamizah
Mohtarrudin, Norhafizah
author_facet Md Roduan, Mohd Rohaizad
Abd Hamid @ Abdul Razak, Roslida
Sulaiman, Hamizah
Mohtarrudin, Norhafizah
author_sort Md Roduan, Mohd Rohaizad
building UPM Institutional Repository
collection Online Access
description Annona muricata, locally known as soursop has been reported to exhibit antiproliferative activities against various cancer cell lines. In this current study, we have investigated the antitumor promotion of various fractions of Annona muricata leaves (AML); hexane (AMLH), dichloromethane (AMLD) and methanol (AMLM) fraction respectively on 7, 12-dimethylbenz[α]anthracene (DMBA) induced and 12-0-tetradecaboylphorbol-13-acetate (TPA) promoted skin tumorigenesis in mice via morphological assessment, biochemical analysis and histopathological evaluation. The results of the study revealed significant inhibition in tumor incidence, tumor burden and tumor volume in the groups received AMLH and AMLD, respectively, and suppressive effects in group received AMLM compared with carcinogen control group at week 21. Superoxide dismutase, catalase, and lipid peroxidation levels were returned to near normal by administration of AML to DMBA/TPA-induced mice. The above findings were supported by histopathological studies, in which the extensive epidermal hyperplasia in carcinogen control group was restored to normal in AML treated groups. Whilst, annonacin, a major annaonaceous acetogenin was found to be the highest in AMLH and AMLD. From the present study, it can be inferred that AML supressed DMBA/TPA-induced skin tumor and this antitumor-promoting activity may be linked to the antioxidant/free radical-scavenging constituents of the extract and annonacin contained in the extracts.
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spelling upm-608682019-03-25T09:43:45Z http://psasir.upm.edu.my/id/eprint/60868/ Annona muricata leaves extracts prevent DMBA/TPA-induced skin tumorigenesis via modulating antioxidants enzyme system in ICR mice Md Roduan, Mohd Rohaizad Abd Hamid @ Abdul Razak, Roslida Sulaiman, Hamizah Mohtarrudin, Norhafizah Annona muricata, locally known as soursop has been reported to exhibit antiproliferative activities against various cancer cell lines. In this current study, we have investigated the antitumor promotion of various fractions of Annona muricata leaves (AML); hexane (AMLH), dichloromethane (AMLD) and methanol (AMLM) fraction respectively on 7, 12-dimethylbenz[α]anthracene (DMBA) induced and 12-0-tetradecaboylphorbol-13-acetate (TPA) promoted skin tumorigenesis in mice via morphological assessment, biochemical analysis and histopathological evaluation. The results of the study revealed significant inhibition in tumor incidence, tumor burden and tumor volume in the groups received AMLH and AMLD, respectively, and suppressive effects in group received AMLM compared with carcinogen control group at week 21. Superoxide dismutase, catalase, and lipid peroxidation levels were returned to near normal by administration of AML to DMBA/TPA-induced mice. The above findings were supported by histopathological studies, in which the extensive epidermal hyperplasia in carcinogen control group was restored to normal in AML treated groups. Whilst, annonacin, a major annaonaceous acetogenin was found to be the highest in AMLH and AMLD. From the present study, it can be inferred that AML supressed DMBA/TPA-induced skin tumor and this antitumor-promoting activity may be linked to the antioxidant/free radical-scavenging constituents of the extract and annonacin contained in the extracts. Elsevier 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/60868/1/Annona%20muricata%20leaves%20extracts%20prevent%20DMBATPA-induced%20skin%20tumorigenesis%20via%20modulating%20antioxidants%20enzyme%20system%20in%20ICR%20mice.pdf Md Roduan, Mohd Rohaizad and Abd Hamid @ Abdul Razak, Roslida and Sulaiman, Hamizah and Mohtarrudin, Norhafizah (2017) Annona muricata leaves extracts prevent DMBA/TPA-induced skin tumorigenesis via modulating antioxidants enzyme system in ICR mice. Biomedicine & Pharmacotherapy, 94. 481 - 488. ISSN 0753-3322; ESSN: 1950-6007 https://reader.elsevier.com/reader/sd/pii/S0753332217325209?token=0BA6E5F770B72152B162EB49C7AD35B1FE36B8DDBB5541CFEBE2DA8DC6708FF58A3D516F2759153F85D35C48974B39A7 10.1016/j.biopha.2017.07.133
spellingShingle Md Roduan, Mohd Rohaizad
Abd Hamid @ Abdul Razak, Roslida
Sulaiman, Hamizah
Mohtarrudin, Norhafizah
Annona muricata leaves extracts prevent DMBA/TPA-induced skin tumorigenesis via modulating antioxidants enzyme system in ICR mice
title Annona muricata leaves extracts prevent DMBA/TPA-induced skin tumorigenesis via modulating antioxidants enzyme system in ICR mice
title_full Annona muricata leaves extracts prevent DMBA/TPA-induced skin tumorigenesis via modulating antioxidants enzyme system in ICR mice
title_fullStr Annona muricata leaves extracts prevent DMBA/TPA-induced skin tumorigenesis via modulating antioxidants enzyme system in ICR mice
title_full_unstemmed Annona muricata leaves extracts prevent DMBA/TPA-induced skin tumorigenesis via modulating antioxidants enzyme system in ICR mice
title_short Annona muricata leaves extracts prevent DMBA/TPA-induced skin tumorigenesis via modulating antioxidants enzyme system in ICR mice
title_sort annona muricata leaves extracts prevent dmba/tpa-induced skin tumorigenesis via modulating antioxidants enzyme system in icr mice
url http://psasir.upm.edu.my/id/eprint/60868/
http://psasir.upm.edu.my/id/eprint/60868/
http://psasir.upm.edu.my/id/eprint/60868/
http://psasir.upm.edu.my/id/eprint/60868/1/Annona%20muricata%20leaves%20extracts%20prevent%20DMBATPA-induced%20skin%20tumorigenesis%20via%20modulating%20antioxidants%20enzyme%20system%20in%20ICR%20mice.pdf