Acute toxicity profiling of the ethyl acetate fraction of Swietenia macrophylla seeds and in-vitro neuroprotection studies

Swietenia macrophylla (SM) is a medicinally important plant found in tropical and subtropical regions of the world. The ethyl acetate fraction of the seeds of S. macrophylla (SMEAF) is reported to exhibit potent anticancer, antitumor, anti-inflammatory and antifeedant activities. Till date, there ha...

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Main Authors: Sayyad, Mustak, Tiang, Ning, Kumari, Yatinesh, Bey, Hing Goh, Jaiswal, Yogini, Rosli, Rozita, Williams, Leonard, Shaikh, Mohd Farooq
Format: Article
Language:English
Published: Elsevier B.V. 2017
Online Access:http://psasir.upm.edu.my/id/eprint/60763/
http://psasir.upm.edu.my/id/eprint/60763/1/Acute%20toxicity%20profiling%20of%20the%20ethyl%20acetate%20fraction%20of%20Swietenia%20macrophylla%20seeds%20and%20in-vitro%20neuroprotection%20studies.pdf
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author Sayyad, Mustak
Tiang, Ning
Kumari, Yatinesh
Bey, Hing Goh
Jaiswal, Yogini
Rosli, Rozita
Williams, Leonard
Shaikh, Mohd Farooq
author_facet Sayyad, Mustak
Tiang, Ning
Kumari, Yatinesh
Bey, Hing Goh
Jaiswal, Yogini
Rosli, Rozita
Williams, Leonard
Shaikh, Mohd Farooq
author_sort Sayyad, Mustak
building UPM Institutional Repository
collection Online Access
description Swietenia macrophylla (SM) is a medicinally important plant found in tropical and subtropical regions of the world. The ethyl acetate fraction of the seeds of S. macrophylla (SMEAF) is reported to exhibit potent anticancer, antitumor, anti-inflammatory and antifeedant activities. Till date, there have been no studies reported on the acute oral toxicity profile of the ethyl acetate fraction of the seeds of SM. The objective of the present study was to determine the acute toxicity of SMEAF and evaluate the in-vitro neuroprotective activity of SMEAF using primary neuronal cell cultures. In acute oral toxicity study, the SMEAF did not produce any lethal signs of morbidity and mortality. Histo-pathological findings, support the safety of SMEAF, as there were no significant changes observed in any of the parameters studied. Based on the results obtained in MTT assay, we infer that SMEAF has a significant neuroprotective effect, as it increased the cell viability and exhibited protection to the neuronal cells against TBHP induced oxidative stress. Thus, SMEAF can be suggested for use in the development of herbal drug formulations with neuroprotective potential.
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spelling upm-607632019-03-28T01:13:40Z http://psasir.upm.edu.my/id/eprint/60763/ Acute toxicity profiling of the ethyl acetate fraction of Swietenia macrophylla seeds and in-vitro neuroprotection studies Sayyad, Mustak Tiang, Ning Kumari, Yatinesh Bey, Hing Goh Jaiswal, Yogini Rosli, Rozita Williams, Leonard Shaikh, Mohd Farooq Swietenia macrophylla (SM) is a medicinally important plant found in tropical and subtropical regions of the world. The ethyl acetate fraction of the seeds of S. macrophylla (SMEAF) is reported to exhibit potent anticancer, antitumor, anti-inflammatory and antifeedant activities. Till date, there have been no studies reported on the acute oral toxicity profile of the ethyl acetate fraction of the seeds of SM. The objective of the present study was to determine the acute toxicity of SMEAF and evaluate the in-vitro neuroprotective activity of SMEAF using primary neuronal cell cultures. In acute oral toxicity study, the SMEAF did not produce any lethal signs of morbidity and mortality. Histo-pathological findings, support the safety of SMEAF, as there were no significant changes observed in any of the parameters studied. Based on the results obtained in MTT assay, we infer that SMEAF has a significant neuroprotective effect, as it increased the cell viability and exhibited protection to the neuronal cells against TBHP induced oxidative stress. Thus, SMEAF can be suggested for use in the development of herbal drug formulations with neuroprotective potential. Elsevier B.V. 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/60763/1/Acute%20toxicity%20profiling%20of%20the%20ethyl%20acetate%20fraction%20of%20Swietenia%20macrophylla%20seeds%20and%20in-vitro%20neuroprotection%20studies.pdf Sayyad, Mustak and Tiang, Ning and Kumari, Yatinesh and Bey, Hing Goh and Jaiswal, Yogini and Rosli, Rozita and Williams, Leonard and Shaikh, Mohd Farooq (2017) Acute toxicity profiling of the ethyl acetate fraction of Swietenia macrophylla seeds and in-vitro neuroprotection studies. Saudi Pharmaceutical Journal, 25 (2). 196 - 205. ISSN 1319-0164; ESSN: 2213-7475 https://pdf.sciencedirectassets.com/278665/1-s2.0-S1319016417X00030/1-s2.0-S1319016416300305/main.pdf?x-amz-security-token=AgoJb3JpZ2luX2VjEGkaCXVzLWVhc3QtMSJHMEUCIQC5oFm7%2FrGQM%2BP3IMwUyIY9EKgVFc1ybOmvAr4DjVH9jgIgJBI63u8uoj6X97bPvEZFD6dXqRG21L77YV4LoqEZ 10.1016/j.jsps.2016.05.002
spellingShingle Sayyad, Mustak
Tiang, Ning
Kumari, Yatinesh
Bey, Hing Goh
Jaiswal, Yogini
Rosli, Rozita
Williams, Leonard
Shaikh, Mohd Farooq
Acute toxicity profiling of the ethyl acetate fraction of Swietenia macrophylla seeds and in-vitro neuroprotection studies
title Acute toxicity profiling of the ethyl acetate fraction of Swietenia macrophylla seeds and in-vitro neuroprotection studies
title_full Acute toxicity profiling of the ethyl acetate fraction of Swietenia macrophylla seeds and in-vitro neuroprotection studies
title_fullStr Acute toxicity profiling of the ethyl acetate fraction of Swietenia macrophylla seeds and in-vitro neuroprotection studies
title_full_unstemmed Acute toxicity profiling of the ethyl acetate fraction of Swietenia macrophylla seeds and in-vitro neuroprotection studies
title_short Acute toxicity profiling of the ethyl acetate fraction of Swietenia macrophylla seeds and in-vitro neuroprotection studies
title_sort acute toxicity profiling of the ethyl acetate fraction of swietenia macrophylla seeds and in-vitro neuroprotection studies
url http://psasir.upm.edu.my/id/eprint/60763/
http://psasir.upm.edu.my/id/eprint/60763/
http://psasir.upm.edu.my/id/eprint/60763/
http://psasir.upm.edu.my/id/eprint/60763/1/Acute%20toxicity%20profiling%20of%20the%20ethyl%20acetate%20fraction%20of%20Swietenia%20macrophylla%20seeds%20and%20in-vitro%20neuroprotection%20studies.pdf