A simple add-and display method for immobilisation of cancer drug on his-tagged virus-like nanoparticles for controlled drug delivery
pH-responsive virus-like nanoparticles (VLNPs) hold promising potential as drug delivery systems for cancer therapy. In the present study, hepatitis B virus (HBV) VLNPs harbouring His-tags were used to display doxorubicin (DOX) via nitrilotriacetic acid (NTA) conjugation. The His-tags served as pH-r...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Publishing Group
2017
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/60732/ http://psasir.upm.edu.my/id/eprint/60732/1/A%20simple%20add-and%20display%20method%20for%20immobilisation%20of%20cancer%20drug%20on%20his-tagged%20virus-like%20nanoparticles%20for%20controlled%20drug%20delivery.pdf |
| _version_ | 1848854262109437952 |
|---|---|
| author | Biabanikhankahdani, Roya Bayat, Saadi Ho, Kok Lian Mohammed Alitheen, Noorjahan Banu Tan, Wen Siang |
| author_facet | Biabanikhankahdani, Roya Bayat, Saadi Ho, Kok Lian Mohammed Alitheen, Noorjahan Banu Tan, Wen Siang |
| author_sort | Biabanikhankahdani, Roya |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | pH-responsive virus-like nanoparticles (VLNPs) hold promising potential as drug delivery systems for cancer therapy. In the present study, hepatitis B virus (HBV) VLNPs harbouring His-tags were used to display doxorubicin (DOX) via nitrilotriacetic acid (NTA) conjugation. The His-tags served as pH-responsive nanojoints which released DOX from VLNPs in a controlled manner. The His-tagged VLNPs conjugated non-covalently with NTA-DOX, and cross-linked with folic acid (FA) were able to specifically target and deliver the DOX into ovarian cancer cells via folate receptor (FR)-mediated endocytosis. The cytotoxicity and cellular uptake results revealed that the His-tagged VLNPs significantly increased the accumulation of DOX in the ovarian cancer cells and enhanced the uptake of DOX, which improved anti-tumour effects. This study demonstrated that NTA-DOX can be easily displayed on His-tagged VLNPs by a simple Add-and-Display step with high coupling efficiency and the drug was only released at low pH in a controlled manner. This approach facilitates specific attachment of any drug molecule on His-tagged VLNPs at the very mild conditions without changing the biological structure and native conformation of the VLNPs. |
| first_indexed | 2025-11-15T11:07:04Z |
| format | Article |
| id | upm-60732 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:07:04Z |
| publishDate | 2017 |
| publisher | Nature Publishing Group |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-607322019-03-28T04:22:19Z http://psasir.upm.edu.my/id/eprint/60732/ A simple add-and display method for immobilisation of cancer drug on his-tagged virus-like nanoparticles for controlled drug delivery Biabanikhankahdani, Roya Bayat, Saadi Ho, Kok Lian Mohammed Alitheen, Noorjahan Banu Tan, Wen Siang pH-responsive virus-like nanoparticles (VLNPs) hold promising potential as drug delivery systems for cancer therapy. In the present study, hepatitis B virus (HBV) VLNPs harbouring His-tags were used to display doxorubicin (DOX) via nitrilotriacetic acid (NTA) conjugation. The His-tags served as pH-responsive nanojoints which released DOX from VLNPs in a controlled manner. The His-tagged VLNPs conjugated non-covalently with NTA-DOX, and cross-linked with folic acid (FA) were able to specifically target and deliver the DOX into ovarian cancer cells via folate receptor (FR)-mediated endocytosis. The cytotoxicity and cellular uptake results revealed that the His-tagged VLNPs significantly increased the accumulation of DOX in the ovarian cancer cells and enhanced the uptake of DOX, which improved anti-tumour effects. This study demonstrated that NTA-DOX can be easily displayed on His-tagged VLNPs by a simple Add-and-Display step with high coupling efficiency and the drug was only released at low pH in a controlled manner. This approach facilitates specific attachment of any drug molecule on His-tagged VLNPs at the very mild conditions without changing the biological structure and native conformation of the VLNPs. Nature Publishing Group 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/60732/1/A%20simple%20add-and%20display%20method%20for%20immobilisation%20of%20cancer%20drug%20on%20his-tagged%20virus-like%20nanoparticles%20for%20controlled%20drug%20delivery.pdf Biabanikhankahdani, Roya and Bayat, Saadi and Ho, Kok Lian and Mohammed Alitheen, Noorjahan Banu and Tan, Wen Siang (2017) A simple add-and display method for immobilisation of cancer drug on his-tagged virus-like nanoparticles for controlled drug delivery. Scientific Reports, 7. pp. 1-12. ISSN 2045-2322 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5509718/pdf/41598_2017_Article_5525.pdf 10.1038/s41598-017-05525-4 |
| spellingShingle | Biabanikhankahdani, Roya Bayat, Saadi Ho, Kok Lian Mohammed Alitheen, Noorjahan Banu Tan, Wen Siang A simple add-and display method for immobilisation of cancer drug on his-tagged virus-like nanoparticles for controlled drug delivery |
| title | A simple add-and display method for immobilisation of cancer drug on his-tagged virus-like nanoparticles for controlled drug delivery |
| title_full | A simple add-and display method for immobilisation of cancer drug on his-tagged virus-like nanoparticles for controlled drug delivery |
| title_fullStr | A simple add-and display method for immobilisation of cancer drug on his-tagged virus-like nanoparticles for controlled drug delivery |
| title_full_unstemmed | A simple add-and display method for immobilisation of cancer drug on his-tagged virus-like nanoparticles for controlled drug delivery |
| title_short | A simple add-and display method for immobilisation of cancer drug on his-tagged virus-like nanoparticles for controlled drug delivery |
| title_sort | simple add-and display method for immobilisation of cancer drug on his-tagged virus-like nanoparticles for controlled drug delivery |
| url | http://psasir.upm.edu.my/id/eprint/60732/ http://psasir.upm.edu.my/id/eprint/60732/ http://psasir.upm.edu.my/id/eprint/60732/ http://psasir.upm.edu.my/id/eprint/60732/1/A%20simple%20add-and%20display%20method%20for%20immobilisation%20of%20cancer%20drug%20on%20his-tagged%20virus-like%20nanoparticles%20for%20controlled%20drug%20delivery.pdf |