Skeletal muscle proteome and meat quality of broiler chickens subjected to gas stunning prior slaughter or slaughtered without stunning

The study examined the effects of pre-slaughter gas stunning and slaughter without stunning on meat quality and skeletal muscle proteome of broiler chickens. Fifty Cobb broiler chickens were randomly assigned to either a neck cut without pre-slaughter stunning (Halal slaughter) or pre-slaughter gas...

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Main Authors: Md Saad, Salwani, Adeyemi, Kazeem Dauda, Zainal Abidin, Siti Aimi Sarah, Palliah, Jaya Vejayan, Idrus, Zulkifli, Sazili, Awis Qurni
Format: Article
Language:English
Published: Taylor & Francis 2016
Online Access:http://psasir.upm.edu.my/id/eprint/60518/
http://psasir.upm.edu.my/id/eprint/60518/1/Skeletal%20muscle%20proteome%20and%20meat%20quality%20of%20broiler%20chickens%20subjected%20to%20gas%20stunning%20prior%20slaughter%20or%20slaughtered%20without%20stunning.pdf
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author Md Saad, Salwani
Adeyemi, Kazeem Dauda
Zainal Abidin, Siti Aimi Sarah
Palliah, Jaya Vejayan
Idrus, Zulkifli
Sazili, Awis Qurni
author_facet Md Saad, Salwani
Adeyemi, Kazeem Dauda
Zainal Abidin, Siti Aimi Sarah
Palliah, Jaya Vejayan
Idrus, Zulkifli
Sazili, Awis Qurni
author_sort Md Saad, Salwani
building UPM Institutional Repository
collection Online Access
description The study examined the effects of pre-slaughter gas stunning and slaughter without stunning on meat quality and skeletal muscle proteome of broiler chickens. Fifty Cobb broiler chickens were randomly assigned to either a neck cut without pre-slaughter stunning (Halal slaughter) or pre-slaughter gas stunning followed by a neck cut. Samples of Pectoralis major muscle at 7 min, 4 h and 24 h postmortem were analyzed for pH, shear force, color, drip and cooking losses. Proteome profile of the 7 min samples was examined by two-dimensional polyacrylamide gel electrophoresis. Birds subjected to Halal slaughter had higher (P < 0.05) redness than those gas stunned at 4 and 24 h postmortem. Gas-stunned birds had lower (P < 0.05) muscle pH and shear force and higher (P < 0.05) drip and cooking losses compared with those subjected to Halal slaughter throughout postmortem storage. Gas stunning up-regulated (P < 0.05) the expression of beta-enolase, pyruvate kinase and creatine kinase compared with Halal slaughter. Results indicate that pre-slaughter gas stunning hastened postmortem energy metabolism and had detrimental effects on the water holding capacity and redness of broiler breast muscles.
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spelling upm-605182018-06-07T08:32:50Z http://psasir.upm.edu.my/id/eprint/60518/ Skeletal muscle proteome and meat quality of broiler chickens subjected to gas stunning prior slaughter or slaughtered without stunning Md Saad, Salwani Adeyemi, Kazeem Dauda Zainal Abidin, Siti Aimi Sarah Palliah, Jaya Vejayan Idrus, Zulkifli Sazili, Awis Qurni The study examined the effects of pre-slaughter gas stunning and slaughter without stunning on meat quality and skeletal muscle proteome of broiler chickens. Fifty Cobb broiler chickens were randomly assigned to either a neck cut without pre-slaughter stunning (Halal slaughter) or pre-slaughter gas stunning followed by a neck cut. Samples of Pectoralis major muscle at 7 min, 4 h and 24 h postmortem were analyzed for pH, shear force, color, drip and cooking losses. Proteome profile of the 7 min samples was examined by two-dimensional polyacrylamide gel electrophoresis. Birds subjected to Halal slaughter had higher (P < 0.05) redness than those gas stunned at 4 and 24 h postmortem. Gas-stunned birds had lower (P < 0.05) muscle pH and shear force and higher (P < 0.05) drip and cooking losses compared with those subjected to Halal slaughter throughout postmortem storage. Gas stunning up-regulated (P < 0.05) the expression of beta-enolase, pyruvate kinase and creatine kinase compared with Halal slaughter. Results indicate that pre-slaughter gas stunning hastened postmortem energy metabolism and had detrimental effects on the water holding capacity and redness of broiler breast muscles. Taylor & Francis 2016 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/60518/1/Skeletal%20muscle%20proteome%20and%20meat%20quality%20of%20broiler%20chickens%20subjected%20to%20gas%20stunning%20prior%20slaughter%20or%20slaughtered%20without%20stunning.pdf Md Saad, Salwani and Adeyemi, Kazeem Dauda and Zainal Abidin, Siti Aimi Sarah and Palliah, Jaya Vejayan and Idrus, Zulkifli and Sazili, Awis Qurni (2016) Skeletal muscle proteome and meat quality of broiler chickens subjected to gas stunning prior slaughter or slaughtered without stunning. CyTA - Journal of Food, 14 (3). pp. 375-381. ISSN 1947-6337; ESSN: 1947-6345 https://www.tandfonline.com/doi/full/10.1080/19476337.2015.1112838 10.1080/19476337.2015.1112838
spellingShingle Md Saad, Salwani
Adeyemi, Kazeem Dauda
Zainal Abidin, Siti Aimi Sarah
Palliah, Jaya Vejayan
Idrus, Zulkifli
Sazili, Awis Qurni
Skeletal muscle proteome and meat quality of broiler chickens subjected to gas stunning prior slaughter or slaughtered without stunning
title Skeletal muscle proteome and meat quality of broiler chickens subjected to gas stunning prior slaughter or slaughtered without stunning
title_full Skeletal muscle proteome and meat quality of broiler chickens subjected to gas stunning prior slaughter or slaughtered without stunning
title_fullStr Skeletal muscle proteome and meat quality of broiler chickens subjected to gas stunning prior slaughter or slaughtered without stunning
title_full_unstemmed Skeletal muscle proteome and meat quality of broiler chickens subjected to gas stunning prior slaughter or slaughtered without stunning
title_short Skeletal muscle proteome and meat quality of broiler chickens subjected to gas stunning prior slaughter or slaughtered without stunning
title_sort skeletal muscle proteome and meat quality of broiler chickens subjected to gas stunning prior slaughter or slaughtered without stunning
url http://psasir.upm.edu.my/id/eprint/60518/
http://psasir.upm.edu.my/id/eprint/60518/
http://psasir.upm.edu.my/id/eprint/60518/
http://psasir.upm.edu.my/id/eprint/60518/1/Skeletal%20muscle%20proteome%20and%20meat%20quality%20of%20broiler%20chickens%20subjected%20to%20gas%20stunning%20prior%20slaughter%20or%20slaughtered%20without%20stunning.pdf