Enzyme immunoassay for the detection of porcine gelatine in edible bird's nests
Porcine gelatine is a common adulterant found in edible bird’s nests (EBNs) used to increase the net weight prior to sale. This study aimed to develop indirect enzyme-linked immunosorbent assays (ELISAs) for porcine gelatine adulteration using anti-peptide polyclonal antibodies. Three indirect ELISA...
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| Format: | Article |
| Language: | English |
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Taylor & Francis
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/60503/ http://psasir.upm.edu.my/id/eprint/60503/1/Enzyme%20immunoassay%20for%20the%20detection%20of%20porcine%20gelatine%20in%20edible%20bird%27s%20nests.pdf |
| _version_ | 1848854213171347456 |
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| author | Tukiran, Nur Azira Ismail, Amin Mustafa, Shuhaimi Hamid, Muhajir |
| author_facet | Tukiran, Nur Azira Ismail, Amin Mustafa, Shuhaimi Hamid, Muhajir |
| author_sort | Tukiran, Nur Azira |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Porcine gelatine is a common adulterant found in edible bird’s nests (EBNs) used to increase the net weight prior to sale. This study aimed to develop indirect enzyme-linked immunosorbent assays (ELISAs) for porcine gelatine adulteration using anti-peptide polyclonal antibodies. Three indirect ELISAs were developed (PAB1, 2 and 3), which had limits of detection (LODs) of 0.12, 0.10 and 0.11 µg g–1, respectively. When applied to standard solutions of porcine gelatine, the inter- and intra-assays showed coefficients of variation (CVs) less than 20% and were able to detect at least 0.5 ng µg–1 (0.05%) porcine gelatine in spiked samples. The proposed ELISA offers attractions for quality control in the EBN industry. |
| first_indexed | 2025-11-15T11:06:17Z |
| format | Article |
| id | upm-60503 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:06:17Z |
| publishDate | 2015 |
| publisher | Taylor & Francis |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-605032018-06-07T06:24:21Z http://psasir.upm.edu.my/id/eprint/60503/ Enzyme immunoassay for the detection of porcine gelatine in edible bird's nests Tukiran, Nur Azira Ismail, Amin Mustafa, Shuhaimi Hamid, Muhajir Porcine gelatine is a common adulterant found in edible bird’s nests (EBNs) used to increase the net weight prior to sale. This study aimed to develop indirect enzyme-linked immunosorbent assays (ELISAs) for porcine gelatine adulteration using anti-peptide polyclonal antibodies. Three indirect ELISAs were developed (PAB1, 2 and 3), which had limits of detection (LODs) of 0.12, 0.10 and 0.11 µg g–1, respectively. When applied to standard solutions of porcine gelatine, the inter- and intra-assays showed coefficients of variation (CVs) less than 20% and were able to detect at least 0.5 ng µg–1 (0.05%) porcine gelatine in spiked samples. The proposed ELISA offers attractions for quality control in the EBN industry. Taylor & Francis 2015 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/60503/1/Enzyme%20immunoassay%20for%20the%20detection%20of%20porcine%20gelatine%20in%20edible%20bird%27s%20nests.pdf Tukiran, Nur Azira and Ismail, Amin and Mustafa, Shuhaimi and Hamid, Muhajir (2015) Enzyme immunoassay for the detection of porcine gelatine in edible bird's nests. Food Additives & Contaminants: Part A, 32 (7). pp. 1023-1028. ISSN 1944-0049; ESSN: 1944-0057 https://www.tandfonline.com/doi/abs/10.1080/19440049.2015.1039605?src=recsys&journalCode=tfac20 10.1080/19440049.2015.1039605 |
| spellingShingle | Tukiran, Nur Azira Ismail, Amin Mustafa, Shuhaimi Hamid, Muhajir Enzyme immunoassay for the detection of porcine gelatine in edible bird's nests |
| title | Enzyme immunoassay for the detection of porcine gelatine in edible bird's nests |
| title_full | Enzyme immunoassay for the detection of porcine gelatine in edible bird's nests |
| title_fullStr | Enzyme immunoassay for the detection of porcine gelatine in edible bird's nests |
| title_full_unstemmed | Enzyme immunoassay for the detection of porcine gelatine in edible bird's nests |
| title_short | Enzyme immunoassay for the detection of porcine gelatine in edible bird's nests |
| title_sort | enzyme immunoassay for the detection of porcine gelatine in edible bird's nests |
| url | http://psasir.upm.edu.my/id/eprint/60503/ http://psasir.upm.edu.my/id/eprint/60503/ http://psasir.upm.edu.my/id/eprint/60503/ http://psasir.upm.edu.my/id/eprint/60503/1/Enzyme%20immunoassay%20for%20the%20detection%20of%20porcine%20gelatine%20in%20edible%20bird%27s%20nests.pdf |