SDS-PAGE and enzyme immunoassay methods for detection of porcine gelatin in edible bird's nest
Porcine gelatin is one of the common adulterant found in processed edible bird’s nest (EBN); as to increase its net weight prior to sale. This study aimed to develop a sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and an enzyme immunoassay method to detect the presence of por...
| Main Authors: | , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
Halal Products Research Institute, Universiti Putra Malaysia
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/60502/ http://psasir.upm.edu.my/id/eprint/60502/1/Mihrec%204.pdf |
| _version_ | 1848854212858871808 |
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| author | Tukiran, Nur Azira Mohamed Roslan, Nur Illiyin Ismail, Amin |
| author_facet | Tukiran, Nur Azira Mohamed Roslan, Nur Illiyin Ismail, Amin |
| author_sort | Tukiran, Nur Azira |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Porcine gelatin is one of the common adulterant found in processed edible bird’s nest (EBN); as to increase its net weight prior to sale. This study aimed to develop a sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and an enzyme immunoassay method to detect the presence of porcine gelatin in processed EBN. SDS-PAGE method was developed by comparing the polypeptide patterns of porcine gelatin with the EBN. In the evaluation of the spiked samples of 2.5% to 50% (v/v), SDS-PAGE was able to detect 20% of porcine gelatin in EBN. An indirect enzyme linked immunosorbent assay (ELISA) was then developed for detection of lower amounts of porcine gelatin. A polyclonal rabbit antibodies was raised against the species-specific sequence of porcine collagen alpha 2 (I) chain. The detection limit for porcine gelatin was 0.05% in EBN. This ELISA is suitable for monitoring of EBN’s quality and purity in the supply chain. |
| first_indexed | 2025-11-15T11:06:17Z |
| format | Conference or Workshop Item |
| id | upm-60502 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:06:17Z |
| publishDate | 2014 |
| publisher | Halal Products Research Institute, Universiti Putra Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-605022018-06-07T06:24:23Z http://psasir.upm.edu.my/id/eprint/60502/ SDS-PAGE and enzyme immunoassay methods for detection of porcine gelatin in edible bird's nest Tukiran, Nur Azira Mohamed Roslan, Nur Illiyin Ismail, Amin Porcine gelatin is one of the common adulterant found in processed edible bird’s nest (EBN); as to increase its net weight prior to sale. This study aimed to develop a sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and an enzyme immunoassay method to detect the presence of porcine gelatin in processed EBN. SDS-PAGE method was developed by comparing the polypeptide patterns of porcine gelatin with the EBN. In the evaluation of the spiked samples of 2.5% to 50% (v/v), SDS-PAGE was able to detect 20% of porcine gelatin in EBN. An indirect enzyme linked immunosorbent assay (ELISA) was then developed for detection of lower amounts of porcine gelatin. A polyclonal rabbit antibodies was raised against the species-specific sequence of porcine collagen alpha 2 (I) chain. The detection limit for porcine gelatin was 0.05% in EBN. This ELISA is suitable for monitoring of EBN’s quality and purity in the supply chain. Halal Products Research Institute, Universiti Putra Malaysia 2014 Conference or Workshop Item PeerReviewed text en http://psasir.upm.edu.my/id/eprint/60502/1/Mihrec%204.pdf Tukiran, Nur Azira and Mohamed Roslan, Nur Illiyin and Ismail, Amin (2014) SDS-PAGE and enzyme immunoassay methods for detection of porcine gelatin in edible bird's nest. In: Malaysia International Halal Research & Education Conference 2014 (MIHREC 2014), 2-4 Dec. 2014, Marriott Putrajaya Hotel, Malaysia. (pp. 34-39). |
| spellingShingle | Tukiran, Nur Azira Mohamed Roslan, Nur Illiyin Ismail, Amin SDS-PAGE and enzyme immunoassay methods for detection of porcine gelatin in edible bird's nest |
| title | SDS-PAGE and enzyme immunoassay methods for detection of porcine gelatin in edible bird's nest |
| title_full | SDS-PAGE and enzyme immunoassay methods for detection of porcine gelatin in edible bird's nest |
| title_fullStr | SDS-PAGE and enzyme immunoassay methods for detection of porcine gelatin in edible bird's nest |
| title_full_unstemmed | SDS-PAGE and enzyme immunoassay methods for detection of porcine gelatin in edible bird's nest |
| title_short | SDS-PAGE and enzyme immunoassay methods for detection of porcine gelatin in edible bird's nest |
| title_sort | sds-page and enzyme immunoassay methods for detection of porcine gelatin in edible bird's nest |
| url | http://psasir.upm.edu.my/id/eprint/60502/ http://psasir.upm.edu.my/id/eprint/60502/1/Mihrec%204.pdf |